Summary
DNAs have been isolated from various Oryza species and studied using physical techniques. The percent of guanine plus cytosine has been determined by thermal denaturation. While the base composition varied between the species, no heterogeneity in the base pair distribution was observed. Renaturation kinetics data of DNAs from different species show that the proportion of repeated DNA sequences vary considerably depending on the DNA content per cell, whereas the nonrepetitive DNA component remains relatively constant. These results suggest that in addition to a small range of DNA variation between the species, changes in the base composition and proportion of repeated sequences have accompanied divergence of the species within the genus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Britten, R.J.: DNA sequence interspersion and a speculation about evolution. Brookhaven Symp. Biol. 23, 80–94 (1972)
Britten, R.J.; Davidson, E.H.: Repetitive and nonrepetitive DNA sequences and a speculation on the evolutionary novelty. Quart. Rev. Biol. 46, 111–133 (1971)
Britten, R.J.; Graham, D.E.; Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in Enzymology 29 E (eds.: Grossman, L. and Moldave, K.), pp. 363–418. New York: Acad. Press 1974
Britten, R.J.; Kohne, D.E.: Repeated sequences in DNA. Science 161, 529–540 (1968)
Britten, R.J.; Smith, J.: A bovine genome. Carnegie Inst. Year Book 68, 378–386 (1970)
Cairns, J.: The chromosome of E. coli. Cold Spring Harbor Symp. Quant. Biol. 28, 43–46 (1963)
Davidson, E.H.; Hough, B.R.; Amenson, C.S.; Britten, R.J.: General interspersion of repetitive with nonrepetitive sequence elements in the DNA of Xenopus. J. Mol. Biol. 77, 1–23 (1973)
Flamm, W.G.: Highly repetitive sequences of DNA in chromosomes. Int. Rev. Cytol. 32, 1–51 (1972)
Flavell, R.B.; Bennett, M.D.; Smith, J.B.; Smith, D.B.: Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269 (1974)
Fry, K.; Poon, R.; Whitcome, P.; Idriss, J.; Salser, W.; Mazrimas, J.A.; Hatch, F.: Nucleotide sequences of HS- β-satellite DNA from kangaroo rat Dipodomys ordii. Proc. Nat. Acad. Aci. U.S., 70, 2642–2646 (1973)
Iyengar, G.A.S.; Sen, S.K.: Nuclear DNA content of several wild and cultivated Oryza species. Environ. Exp. Bot. (in press)
Kramm, R.; Botchan, M.; Hearst, J.E.: Arrangement of the highly reiterated sequences in the centric heterochromatin of Drosophila melanogaster: Evidence for interspersed spacer DNA. J. Mol. Biol. 64, 103–117 (1972)
Laird, C.D.: Chromatid structure: Relationship between DNA content and nucleotide sequence diversity. Chromosoma 32, 378–406 (1971)
Macgregor, H.C.; Horner, H.; Owen, C.A.; Parker, I.: Observations on heterochromatin and satellite DNA in salamanders of the genus Plethodon. Chromosoma 43, 329–348 (1973)
McCarthy, B.J.; Farquhar, M.N.: The rate of change of DNA in evolution. Brokhaven Symp. Biol. 23, 1–43 (1972)
Mandel, M.; Marmur, J.: Use of Ultraviolet absorbance temperature profile for determining guanine plus cytosine content in DNA. In: Methods in Enzymology 12 B (eds.: Grossman, L.; Moldave, K.), pp. 195–206. New York: Acad. Press 1968
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208–218 (1961)
Miyazawa, Y.; Thomas, C.A.: Nucleotide composition of short sequences of DNA molecules. J. Mol. Biol. 11, 223–237 (1965)
Nayar, N.M.: Origin and cytogenetics of rice. Adv. Genet. 17, 153–292 (1973)
Pardue, M.L.; Gall, J.G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970)
Rice, N.R.: Changes in repeated sequence DNA in evolution. Brookhaven Symp. Biol. 23, 44–79 (1972)
Smith, D.B.; Flavell, R.B.: Relatedness and evolution of repeated nucleotide sequences in genomes of some Gramineae species. Biochem. Genet. 12, 243–256 (1974)
Strauss, N.A.: Comparative renaturation kinetics of amphibians. Proc. Nat. Acad. Sci. (USA) 68, 799–802 (1971)
Southern, E.M.: Base sequence and evolution of guinea-pig-satellite DNA. Nature 227, 794–798 (1970)
Van der Schans, G.P.; Allen, J.B.T.; Block, J.: Determination of molecular weight distributions of DNA by means of sedimentation in a sucrose gradient. Anal. Biochem. 32, 14–30 (1969)
Wu, J.R.; Hum, J.; Bonner, J.: Size and distribution of the repetitive sequences of the Drosophila genome. J. Mol. Biol. 64, 211–219 (1972)
Author information
Authors and Affiliations
Additional information
Communicated by D. von Wettstein
Rights and permissions
About this article
Cite this article
Iyengar, G.A.S., Gaddipati, J.P. & Sen, S.K. Characteristics of nuclear DNA in the genus Oryza . Theoret. Appl. Genetics 54, 219–224 (1979). https://doi.org/10.1007/BF00267711
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00267711