Summary
Escherichia coli strains with preexisting ribosomal mutations were used in order to isolate further ribosomal mutations. The ribosomal mutations used were resistance to erythromycin, spectinomycin, streptomycin or kasugamycin. These mutations cause alteration of specific ribosomal elements, L4, S5, S12 proteins and 16S rRNA respectively. Mutations have been introduced into strains carrying one, two or three of these mutations. Strains with all possible combinations of these four mutations were constructed. The phenotypes of all isolated mutants were tested, and frequently the strains lost one or more of their pre-existing resistances.
Thus, functional interactions were revealed among proteins, as well as RNA and proteins within the 30 S ribosomal subunit and as well as between the 30 S and the 50 S ribosomal subunits.
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Communicated by H. G. Wittmann
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Saltzman, L.A., Brown, M. & Apirion, D. Functional interdependence among ribosomal elements as revealed by genetic analysis. Molec. Gen. Genet. 133, 201–207 (1974). https://doi.org/10.1007/BF00267669
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DOI: https://doi.org/10.1007/BF00267669