Summary
MutD5, a conditional mutator in Escherichia coli, causes the stimulation of mutation frequencies 50 to 100 fold in minimal medium. In rich medium mutation frequencies are further increased 50 to 100 fold. We show here that all possible base-pair mutations are increased in a mutD5 strain grown in rich medium. A:T↔G:C transitions as well as A:T↔C:G, A:T↔T:A aud G:C↔C:G transversions are stimulated. Transitions occur more frequently than transversions. MutD5 also increases the reversion frequencies of three trpA frameshift mutations by causing base-pair additions, and, possibly, base-pair deletions.
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Allen, M. K., Yanofsky, C.: A biochemical and genetic study of reversion with the A-gene A-protein system of Escherichia coli tryptophan synthetase. Genetics 48, 1065–1083 (1963)
Berger, H., Brammar, W. J., Yanofsky, C.: Spontaneous and ICR191-A induced frameshift mutations in the A gene of Escherichia coli tryptophan synthetase. J. Bact. 96, 1672–1679 (1968)
Berger, H., Yanofsky, C.: Suppressor selection for amino acid replacements expected on the basis of the genetic code. Science 156, 394–397 (1967)
Brammar, W. J.: Independent expression of the A gene of the tryptophan operon of Escherichia coli during tryptophan starvation. J. gen. Microbiol. 76, 395–405 (1973)
Brammar, W. J., Berger, H., Yanofsky, C.: Altered amino acid sequences produced by reversion of frameshift mutants of tryptophan synthetase A gene of E. coli. Proc. nat. Acad. Sci. (Wash.) 58, 1499–1506 (1967)
Brutlag, D., Kornberg, A.: Enzymatic synthesis of DNA. XXXVI. A proofreading function for the 3′–5′ exonuclease activity in DNA polymerases. J. biol. Chem. 247, 241–248 (1972)
Cox, E. C., Degnen, G. E., Scheppe, M. L.: Mutator gene studies in Escherichia coli: The mutS gene. Genetics 72, 551–567 (1972)
Cox, E. C., Yanofsky, C.: Altered base ratios in the DNA of an Escherichia coli mutator strain. Proc. nat. Acad. Sci. (Wash.) 58, 1895–1902 (1967)
Crick, F. H. C.: Codon-anticodon pairing: The Wobble hypothesis. J. molec. Biol. 19, 548–555 (1966)
Degnen, G. E., Cox, E. C.: A conditional mutator gene in Escherichia coli: Isolation, mapping and effector studies. J. Bact. 117, 477–487 (1974)
Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, P. E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966)
Drapeau, G. R., Brammar, W. J., Yanofsky, C.: Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of Escherichia coli. J. molec. Biol. 35, 395–405 (1973)
Fresco, J. R., Alberts, B. M.: The accommodation of noncomplementary bases in helical polyribonucleotides and deoxyribonucleic acids. Proc. nat. Acad. Sci. (Wash.) 46, 311–321 (1960)
Hall, R. M., Brammar, W. J.: Increased spontaneous mutation rates in mutants of E. coli with altered DNA polymerase III. Molec. gen. Genet. 121, 271–276 (1973)
Lennox, E. S.: Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1, 190–206 (1955)
Lester, G., Yanofsky, C.: Influence of 3-methylanthranilic and anthranilic acids on the formation of tryptophan synthetase in Escherichia coli. J. Bact. 81, 81–90 (1961)
Newton, A., Masys, D., Leonardi, E., Wygal, D.: Association of induced frameshift mutagenesis and DNA replication in Escherichia coli. Nature (Lond.) new Biol. 236, 19–22 (1972)
Pardee, A. B., Jacob, F., Monod, J.: The genetic control and cytoplasmic expression of “inducibility” in the synthesis of β-galactosidase by E. coli. J. molec. Biol. 1, 165–178 (1959)
Siegel, E. C.: Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J. Bact. 113, 145–160 (1973)
Siegel, E. C., Bryson, V.: Mutator gene of Escherichia coli B. J. Bact. 94, 38–47 (1967)
Siegel, E. C., Kamel, F.: Reversion of frameshift mutations by mutator genes in Escherichia coli. J. Bact. 117, 994–1001 (1974)
Streisinger, G., Okada, Y., Emrich, J., Newton, J., Tsugita, A., Terzaghi, E., Inouye, M.: Frameshift mutations and the genetic code. Cold Spr. Harb. Symp. quant. Biol. 31, 77–84 (1966)
Treffers, H. P., Spinelli, V., Belser, N. O.: A factor (or mutator gene) influencing mutation rates in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 40, 1064–1071 (1954)
Vogel, H. J., Bonner, D. M.: Acetylornithinase of Escherichia coli: Partial purification and some properties. J. biol. Chem. 218, 97–106 (1956)
Yanofsky, C., Carlton, B. C., Guest, J. R., Helinski, D. R., Henning, U.: On the colinearity of gene structure and protein structure. Proc. nat. Acad. Sci. (Wash.) 51, 266–272 (1964)
Yanofsky, C., Cox, E. C., Horn, V. D.: The unusual mutagenic specificity of an Escherichia coli mutator gene. Proc. nat. Acad. Sci. (Wash.) 55, 274–281 (1966a)
Yanofsky, C., Ito, J., Horn, V. D.: Amino acid replacements and the genetic code. Cold Spr. Harb. Symp. quant. Biol. 31, 151–162 (1966b)
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Fowler, R.G., Degnen, G.E. & Cox, E.C. Mutational specificity of a conditional Escherichia coli mutator, mutD5 . Molec. Gen. Genet. 133, 179–191 (1974). https://doi.org/10.1007/BF00267667
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DOI: https://doi.org/10.1007/BF00267667