Summary
In Neurospora crassa, mutants resistant against cycloheximide appear with a marked time lag after mutation induction. We have suggested (Neuhäuser et al., 1970) that this lag indicates the time needed for the synthesis of altered ribosomes (phenotypic lag), that the drug in the wildtype acts upon the ribosomes, and that resistance is due to alterations in them.
By measurements of poly-U directed poly-Phe synthesis on ribosomes of the wildtype and two different cycloheximide resistant mutants in a cell free system it is shown here that mutant ribosomes indeed differ from those of the wildtype. Poly-Phe synthesis on mutant ribosomes proceeds in the presence of the drug, whereas that on wildtype ribosomes is inhibited. This means that the earlier suggestions are correct.
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Abbreviations
- CHX:
-
cycloheximide
References
Fisher, R. S., Yates, F.: Statistical tables for biological, agricultural and medical research, 4th ed., p. 66. New York: Hafner Publishing Co. 1953
Küntzel, H.: Specificity of mitochondrial and cytoplasmic ribosomes from Neurospora crassa in poly-U dependent cell free systems. FEBS Letters 4, 140–142 (1969)
Neuhäuser, A., Klingmüller, W., Kaudewitz, F.: Selektion Actidion-resistenter Mutanten bei Neurospora crassa sowie ihre genetische und biochemische Analyse. Molec. gen. Genet. 106, 180–194 (1970)
Weiss, H., Jagow, G. von, Klingenberg, M., Bücher, Th. Characterization of Neurospora crassa mitochondria prepared with a grind-mill. Europ. J. Biochem. 14, 75–82 (1970)
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Communicated by W. Gajewsky
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Pongratz, M., Klingmüller, W. Role of ribosomes in cycloheximide resistance of Neurospora mutants. Molec. Gen. Genet. 124, 359–363 (1973). https://doi.org/10.1007/BF00267664
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DOI: https://doi.org/10.1007/BF00267664