Skip to main content

Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives

Summary

The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. ‘Hot spots’ for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a ‘hot spot’, since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.

This is a preview of subscription content, access via your institution.

References

  • Achtman, M., Willetts, N., Clark, A.J.: Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterisation of transfer-deficient mutants. J. Bact. 106 529–538 (1971)

    Google Scholar 

  • Barth, P.T., Grinter, N.J.: Map of plasmid RP4 derived by insertion of transposon C. J. molec. Biol. 113, 455–474 (1977)

    Google Scholar 

  • Bennett, P.M., Grinsted, J., Choi, C.L., Richmond, M.H.: Characterisation of Tn501, a transposon determining resistance to mercuric ions. Molec. gen. Genet. 159, 101–106 (1978)

    Google Scholar 

  • Bennett, P.M., Grinsted, J., Richmond, M.H.: Transposition of TnA does not generate deletions. Molec. gen. Genet. 154, 205–211 (1977)

    Google Scholar 

  • Bennett, P.M., Richmond, M.H.: Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Escherichia coli. J. Bact. 126, 1–6 (1976)

    Google Scholar 

  • Botstein, D., Kleckner, N.: Translocation and illegitimate recombination by the tetracycline resistance element Tn10. In: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S.L. Adhya, eds.) pp. 185–203. Cold Spring Harbour: Cold Spring Harbor Laboratory 1977

    Google Scholar 

  • Campbell, A.M.: Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. Biochem. J. 155, 101–105 (1976)

    Google Scholar 

  • Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature (Lond.) 263, 731–738 (1976)

    Google Scholar 

  • Cohen, S.N., Kopecko, D.J.: Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions. Fed. Proc. 35, 2031–2036 (1976)

    Google Scholar 

  • Dougan, G., Sherratt, D.: The transposon Tn1 as a probe for studying Col E1 structure and function. Molec. gen. Genet. 151, 151–160 (1977)

    Google Scholar 

  • Foster, T.J.: Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Molec. gen. Genet. 154, 305–309 (1977)

    Google Scholar 

  • Grinsted, J., Bennett, P.M., Richmond, M.H.: A restriction enzyme map of the R-plasmid RP1. Plasmid 1, 34–37 (1977)

    Google Scholar 

  • Grinsted, J., Saunders, J.R., Ingram, L.C., Sykes, R.B., Richmond, M.H.: Porperties of an R factor that originated in Pseudomonas aeruginosa 1822. J. Bact. 110, 529–537 (1972)

    Google Scholar 

  • Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. nat. Acad. Sci. (Wash.) 72, 3623–3627 (1975)

    Google Scholar 

  • Inselburg, J.: Isolation, mapping and examination of effects of TnA insertion in Col E1 plasmids. J. Bact. 129, 482–491 (1977)

    Google Scholar 

  • Kleckner, N.: Translocatable elements in procaryotes. Cell 11, 11–23 (1977)

    Google Scholar 

  • Kopecko, D.J., Brevet, J., Cohen, S.N.: Involvement of multiple translocating DNA segments and recombinational hot spots in the structural evolution of bacterial plasmids. J. molec. Biol. 108, 333–360 (1976)

    Google Scholar 

  • Kretschmer, P.J., Cohen, S.N.: Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J. Bact. 130, 888–899 (1977)

    Google Scholar 

  • Meyer, R.J., Helinski, D.R.: Undirectional replication of the P-group plasmid RK2. Biochim. biophys. Acta (Amst.) 478, 109–113 (1977)

    Google Scholar 

  • Nisen, P.D., Kopecko, D.J., Chou, J., Cohen, S.N.: Sitespecific DNA deletion occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J. molec. Biol. 117, 975–998 (1977)

    Google Scholar 

  • Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, III, R., Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: a proposal. Bact. Rev. 40, 168–189 (1976)

    Google Scholar 

  • Petrocheilou, V., Grinsted, J., Richmond, M.H.: R plasmid transfer in vivo in the absence of antibiotic selection pressure. Antimicrob. Ag. Chemo. 10, 753–761 (1976)

    Google Scholar 

  • Rubens, C., Heffron, F., Falkow, S.: Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion. J. Bact.128, 425–434 (1976)

    Google Scholar 

  • So, M., Gill, R., Falkow, S.: The generation of a Col El-ApR cloning vehicle which allows detection of inserted DNA. Molec. gen. Genet. 142, 239–249 (1975)

    Google Scholar 

  • Stanisich, V.A., Bennett, P.M., Richmond, M.H.: Characterisation of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J. Bact. 129, 1227–1223 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by W. Arber

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Grinsted, J., Bennett, P.M., Higginson, S. et al. Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Molec. Gen. Genet. 166, 313–320 (1978). https://doi.org/10.1007/BF00267624

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267624

Keywords

  • Regional Preference
  • Tn801 Element