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Molecular and General Genetics MGG

, Volume 166, Issue 3, pp 313–320 | Cite as

Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives

  • J. Grinsted
  • P. M. Bennett
  • S. Higginson
  • M. H. Richmond
Article
  • 32 Downloads

Summary

The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. ‘Hot spots’ for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a ‘hot spot’, since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.

Keywords

Regional Preference Tn801 Element 
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References

  1. Achtman, M., Willetts, N., Clark, A.J.: Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterisation of transfer-deficient mutants. J. Bact. 106 529–538 (1971)Google Scholar
  2. Barth, P.T., Grinter, N.J.: Map of plasmid RP4 derived by insertion of transposon C. J. molec. Biol. 113, 455–474 (1977)Google Scholar
  3. Bennett, P.M., Grinsted, J., Choi, C.L., Richmond, M.H.: Characterisation of Tn501, a transposon determining resistance to mercuric ions. Molec. gen. Genet. 159, 101–106 (1978)Google Scholar
  4. Bennett, P.M., Grinsted, J., Richmond, M.H.: Transposition of TnA does not generate deletions. Molec. gen. Genet. 154, 205–211 (1977)Google Scholar
  5. Bennett, P.M., Richmond, M.H.: Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Escherichia coli. J. Bact. 126, 1–6 (1976)Google Scholar
  6. Botstein, D., Kleckner, N.: Translocation and illegitimate recombination by the tetracycline resistance element Tn10. In: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S.L. Adhya, eds.) pp. 185–203. Cold Spring Harbour: Cold Spring Harbor Laboratory 1977Google Scholar
  7. Campbell, A.M.: Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. Biochem. J. 155, 101–105 (1976)Google Scholar
  8. Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature (Lond.) 263, 731–738 (1976)Google Scholar
  9. Cohen, S.N., Kopecko, D.J.: Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions. Fed. Proc. 35, 2031–2036 (1976)Google Scholar
  10. Dougan, G., Sherratt, D.: The transposon Tn1 as a probe for studying Col E1 structure and function. Molec. gen. Genet. 151, 151–160 (1977)Google Scholar
  11. Foster, T.J.: Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Molec. gen. Genet. 154, 305–309 (1977)Google Scholar
  12. Grinsted, J., Bennett, P.M., Richmond, M.H.: A restriction enzyme map of the R-plasmid RP1. Plasmid 1, 34–37 (1977)Google Scholar
  13. Grinsted, J., Saunders, J.R., Ingram, L.C., Sykes, R.B., Richmond, M.H.: Porperties of an R factor that originated in Pseudomonas aeruginosa 1822. J. Bact. 110, 529–537 (1972)Google Scholar
  14. Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. nat. Acad. Sci. (Wash.) 72, 3623–3627 (1975)Google Scholar
  15. Inselburg, J.: Isolation, mapping and examination of effects of TnA insertion in Col E1 plasmids. J. Bact. 129, 482–491 (1977)Google Scholar
  16. Kleckner, N.: Translocatable elements in procaryotes. Cell 11, 11–23 (1977)Google Scholar
  17. Kopecko, D.J., Brevet, J., Cohen, S.N.: Involvement of multiple translocating DNA segments and recombinational hot spots in the structural evolution of bacterial plasmids. J. molec. Biol. 108, 333–360 (1976)Google Scholar
  18. Kretschmer, P.J., Cohen, S.N.: Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J. Bact. 130, 888–899 (1977)Google Scholar
  19. Meyer, R.J., Helinski, D.R.: Undirectional replication of the P-group plasmid RK2. Biochim. biophys. Acta (Amst.) 478, 109–113 (1977)Google Scholar
  20. Nisen, P.D., Kopecko, D.J., Chou, J., Cohen, S.N.: Sitespecific DNA deletion occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J. molec. Biol. 117, 975–998 (1977)Google Scholar
  21. Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, III, R., Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: a proposal. Bact. Rev. 40, 168–189 (1976)Google Scholar
  22. Petrocheilou, V., Grinsted, J., Richmond, M.H.: R plasmid transfer in vivo in the absence of antibiotic selection pressure. Antimicrob. Ag. Chemo. 10, 753–761 (1976)Google Scholar
  23. Rubens, C., Heffron, F., Falkow, S.: Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion. J. Bact.128, 425–434 (1976)Google Scholar
  24. So, M., Gill, R., Falkow, S.: The generation of a Col El-ApR cloning vehicle which allows detection of inserted DNA. Molec. gen. Genet. 142, 239–249 (1975)Google Scholar
  25. Stanisich, V.A., Bennett, P.M., Richmond, M.H.: Characterisation of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J. Bact. 129, 1227–1223 (1977)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • J. Grinsted
    • 1
  • P. M. Bennett
    • 1
  • S. Higginson
    • 1
  • M. H. Richmond
    • 1
  1. 1.Department of BacteriologyUniversity of BristolBristolEngland

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