Molecular and General Genetics MGG

, Volume 166, Issue 3, pp 313–320 | Cite as

Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives

  • J. Grinsted
  • P. M. Bennett
  • S. Higginson
  • M. H. Richmond


The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. ‘Hot spots’ for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a ‘hot spot’, since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.


Regional Preference Tn801 Element 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • J. Grinsted
    • 1
  • P. M. Bennett
    • 1
  • S. Higginson
    • 1
  • M. H. Richmond
    • 1
  1. 1.Department of BacteriologyUniversity of BristolBristolEngland

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