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Map location of the Escherichia coli origin of replication

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Summary

Working with restriction fragments obtained directly from the Escherichia coli K12 chromosome, the EcoRI-HindIII restriction map of the section of the chromosome containing the replication origin has been extended by 14 kilobase pairs (kb) to cover 56kb. Within this newly mapped portion, the liv and rrnC cistrons have been identified by (1) hybridization of individual restriction fragmenents to the ilv-transducing phage λdilv5 and (2) a comparison of the restriction map of this region with the EcoRI map of λdilv5 and the HindIII map of the plasmid pJC110, a ColE1-ilv hybrid. The replication origin is located approximately 30 kb from the ilvE gene and 20 kb from the rrnC 16S rRNA cistron. This places the origin near 82.7 min on the genetic map, close to uncA.

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References

  • Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)

    Google Scholar 

  • Bird, R.E., Louran, J., Martuscelli, J., Caro, L.G.: Origin and sequence of chromosome replication in Escherichia coli. J. molec. Biol 70, 549–556 (1972)

    Google Scholar 

  • Blin, N., Gabain, A.V., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Letters 53, 84–86 (1975)

    Google Scholar 

  • Bonner, W.M., Laskey, R.A.: A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels. Europ. J. Biochem. 46, 83–88 (1974)

    Google Scholar 

  • Carl, P.: Escherichia coli mutants with temperature-sensitive synthesis of DNA. Molec. gen. Genet. 109, 107–122 (1970)

    Google Scholar 

  • Clewell, D.B.: Nature of ColE1 plasmid replication in E. coli in the presence of chloramphenicol. J. Bact. 110, 667–676 (1972)

    Google Scholar 

  • Collins, J., Fiil, N.P., Jørgensen, P., Friesen, J.D.: Gene cloning of Escherichia coli chromosomal genes important in the regulation of ribosomal RNA synthesis. In: Alfred Benzon Symposium IX. Control of Ribosome Synthesis, pp 356–367. Copenhagen: Munksgaard 1976

    Google Scholar 

  • Freifelder, D.: Preparation of covalently closed and open circular DNA molecules of phage lambda. Biochem. biophys. Acta (Amst.) 432, 113–117 (1976)

    Google Scholar 

  • Hiraga, S.: Novel F prime factors able to replicate in Escherichia coli Hfr strains. Proc. nat. Acad. Sci. (Wash.) 73, 198–202 (1976)

    Google Scholar 

  • Hohlfeld, R., Vielmetter, W.: Bidirectional growth of the Escherichia coli chromosome. Nature (Lond.) New Biol 242, 130–132 (1973)

    Google Scholar 

  • Jonasson, J.: Evidence for bidirectional chromosome replication in Escherichia coli C based on marker-frequency analysis by DNA/DNA hybridization with P2 and λ prophages. Molec. gen. Genet. 120, 69–90 (1973)

    Google Scholar 

  • Kenerley, M.E., Morgan, E.A., Post, L., Lindahl, L., Nomura, M.: Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli. J. Bact. 132, 931–949 (1977)

    Google Scholar 

  • Louarn, J., Funderburgh, M., Bird, R.E.: More precise mapping of the replication origin in Escherichia coli K-12. J. Bact. 120, 1–5 (1974)

    Google Scholar 

  • Marsh, R.C., Worcel, A.: A DNA fragment containing the origin of replication of the Escherichia coli chromosome. Proc. nat. Acad. Sci. (Wash.) 74, 2720–2724 (1977)

    Google Scholar 

  • Masters, M.: Strains of Escherichia coli diploid for the chromosomal origin of DNA replication. Molec. gen. Genet. 143, 105–111 (1975)

    Google Scholar 

  • Masters, M., Broda, P.: Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature (Lond.) New Biol. 232, 137–140 (1971)

    Google Scholar 

  • Meyenburg, K. von, Hansen, F.G., Nielsen, L.D., Jørgensen, P.: Origin of replication, oriC, of the Escherichia coli chromosome: Mapping of genes relative to the R-EcoRI cleavage sites in the oriC region. Molec. gen. Genet. 158, 101–109 (1977)

    Google Scholar 

  • McCorkle, G.M., Leathers, T.D., Umbarger, H.E.: Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc. nat. Acad. Sci. (Wash.) 75, 89–93 (1978)

    Google Scholar 

  • Morgan, E.A., Ikemura, T., Nomura, M.: Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 74, 2710–2714 (1977)

    Google Scholar 

  • Prescott, D.M., Kuempel, P.L.: Bidirectional replication of the chromosome in Escherichia coli. Pric. nat. Acad. Sci. (Wash.) 69, 2842–2845 (1972)

    Google Scholar 

  • Rodriguez, R.L., Dalbery, M.S., Davern, C.I.: Autoradiographic evidence for bidirectional DNA replication in Escherichia coli. J. molec. Biol. 74, 599–604 (1973)

    Google Scholar 

  • Schwartz, M., Worcel, A.: Reinitiation of chromosome replication in a thermosensitive DNA mutant of Escherichia coli. II. Synchronization of chromosome replication after temperature shifts. J. molec. Biol. 61, 329–342 (1971)

    Google Scholar 

  • Sugden, B., DeTroy, B., Roberts, R.J., Sambrook, J.: Agarose slab gel electrophoresis equipment. Anlyt. Biochem. 68, 36–46 (1975)

    Google Scholar 

  • Wada, C., Yura, T., Hiraga, S.: Replication of Fpoh +plasmid in mafA mutants of Escherichia coli defective in plasmid maintenance. Molec. gen. Genet. 152, 211–217 (1977)

    Google Scholar 

  • Yasuda, S., Hirota, Y.: Cloning and mapping of the replication origin of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 74, 5458–5462 (1977)

    Google Scholar 

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Authors and Affiliations

  1. Biology Programs, The University of Texas at Dallas, P.O. Box 688, 75080, Richardson, Texas, USA

    Robert C. Marsh

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  1. Robert C. Marsh
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Communicated by W. Arber

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Marsh, R.C. Map location of the Escherichia coli origin of replication. Molec. Gen. Genet. 166, 299–304 (1978). https://doi.org/10.1007/BF00267622

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  • DOI: https://doi.org/10.1007/BF00267622

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