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Specific labelling of replicating SPP1 DNA

Analysis of viral DNA synthesis and identification of phage dna-genes

Summary

Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SPP1 involved in phage DNA synthesis and to define intermediates in SPP1 replication.

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Experiments reported were part of the Doctoral Thesis submitted by K. Burger to the Freie Universität Berlin

Communicated by E. Bautz

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Burger, K.J., Trautner, T.A. Specific labelling of replicating SPP1 DNA. Molec. Gen. Genet. 166, 277–285 (1978). https://doi.org/10.1007/BF00267619

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  • DOI: https://doi.org/10.1007/BF00267619

Keywords

  • Subtilis Strain
  • Specific Labelling
  • Nonpermissive Temperature
  • Initation Mutation
  • Bacteriophage SPP1