Skip to main content

Specific labelling of replicating SPP1 DNA

Analysis of viral DNA synthesis and identification of phage dna-genes

Molecular and General Genetics MGG volume 166, pages 277–285 (1978)Cite this article

Summary

Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SPP1 involved in phage DNA synthesis and to define intermediates in SPP1 replication.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Bazill, G., Gross, J.: Effect of 6-(p-hydroxyphenylazo)-uracil on B. subtilis DNA polymerase. Nature (Lond.) New Biol. 240, 82–83 (1972)

    Google Scholar 

  • Boyce, R.P., Setlow, R.B.: A simple method of increasing the incorporation of thymidine into the deoxyribonucleic acid of Escherichia coli. Biochem. biophys. Acta (Amst.) 61, 618–620 (1962)

    Google Scholar 

  • Burger, K.: Biochemische und genetische Untersuchungen zur DNA-Replikation des B. subtilis Bakteriophagen SPP1. Doctoral Thesis, Freie Universität Berlin, 1978

  • Canosi, U., Siccardi, A.G., Falaschi, A., Mazza, G.: Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. J. Bact. 126, 108–121 (1976)

    Google Scholar 

  • Clements, J.E., D'Ambrosio, J., Brown, N.C.: Inhibition of Bacillus subtilis deoxyribonucleic acid polymerase by phenylhydrazinopyrimidines. Demonstration of a drug-induced deoxyribonucleic acid-enzyme complex. J. biol. Chem. 250, 522–526 (1975)

    Google Scholar 

  • Cozzarelli, N., Low, R.: Mutational alteration of Bacillus subtilis DNA polymerase III to hydroxyphenyl-azopyrimidine resistance: polymerase III is necessary for DNA replication. Biochem. biophys. Res. Commun. 51, 151–157 (1973)

    Google Scholar 

  • Esche, H., Schweiger, M., Trautner, T.A.: Gene expression of bacteriophage SPP1. I. Phage directed protein synthesis. Molec. gen. Genet. 142, 45–55 (1975)

    Google Scholar 

  • Esche, H.: Gene expression of bacteriophage SPP1. II. Regulatory aspects. Molec. gen. Genet. 142, 57–66 (1975)

    Google Scholar 

  • Esche, H., Spatz, H. Ch.: Asymmetric transcription of SPP1 in vivo. Molec. gen. Genet. 124, 57–63 (1973)

    Google Scholar 

  • Ganesan, A.T., Andersen, J.J., Luh, J., Effron, M.: DNA metabolism in Bacillus subtilis and its phage SPP1. In: Microbiology 1976 (D. Schlessinger, ed.), pp. 319–325. Washington D.C.: American Society for Microbiology 1976

    Google Scholar 

  • Gass, K.B., Low, R.L., Cozzarelli, N.R.: Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines. Proc. natl. Acad. Sci. (Wash.) 70, 103–107 (1973)

    Google Scholar 

  • Georgopoulos, C.P.: Suppressor systems in B. subtilis 168. J. Bact. 97, 1397–1402 (1969)

    Google Scholar 

  • Hemphill, H.E., Whiteley, A.R.: Bacteriophages of Bacillus subtilis. Bact. Rev. 39, 257–315 (1975)

    Google Scholar 

  • Kahmann, R.: Das Verhalten von SPP1 DNA nach Transfektion von B. subtilis. Doctoral Thesis, Freie Universität Berlin, 1974

  • Mackenzie, J.M., Neville, M.N., Wright, G.E., Brown, N.C.: Hydroxyphenylazopyrimidines: characterization of the active forms and their inhibitory action on a DNA polymerase from Bacillus subtilis. Proc. nat. Acad. Sci (Wash.) 70, 512–516 (1973)

    Google Scholar 

  • Mastromei, G., Riva, S., Fietta, A., Pagani, L.: SPP1 DNA replicative forms: Growth of phage SPP1 in Bacillus subtilis mutants temperature-sensitive in DNA Synthesis. Molec. gen. Genet. (Submitted)

  • Mendelson, N.H., Gross, J.D.: Characterization of a temperaturesensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication. J. Bact. 94, 1603–1608 (1967)

    Google Scholar 

  • Milanesi, G., Cassani, G.: Transcription after bacteriophage SPP1 infection in Bacillus subtilis. J. Virol. 10, 187–192 (1972)

    Google Scholar 

  • Montenegro, M., Esche, H., Trautner, T.A.: Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III. Molec. gen. Genet. 149, 131–134 (1976)

    Google Scholar 

  • Morelli, G., Montenegro, M.A., Hillenbrandt, G., Scherzinger, E., Trautner, T.A.: The genome of B. subtilis phase SPP1: Assignment of 5′–3′-Polarity to the complementary strands of SPP1 DNA. Molec. gen. Genet. (1978) (in press)

  • Neville, M.N., Brown, N.C.: Inhibition of a discrete bacterial DNA polymerase by 6 p-(hydroxyphenylazo)-uracil and 6 p-(hydroxyphenylazo)-isocytosine. Nature (Lond.) New Biol. 240, 80–81 (1972)

    Google Scholar 

  • Riva, S., Polsinelli, M., Falaschi, A.: A new phage of Bacillus subtilis with infectious DNA having separable strands. J. molec. Biol. 35, 347–356 (1968)

    Google Scholar 

  • Rottländer, E., Trautner, T.A.: Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain 168. Molec. gen. Genet. 108, 47–60 (1970)

    Google Scholar 

  • Rowley, S.D., Brown, N.C.: Bacillus subtilis DNA polymerase III is required for the replication of DNA of bacteriophages SPP1 and Φ105. J. Virol. 21, 493–496 (1977)

    Google Scholar 

  • Spatz, H. Ch., Trautner, T.A.: One way to do experiments on gene conversion? Molec. gen. Genet. 109, 84–106 (1970)

    Google Scholar 

  • Spatz, H.Ch., Trautner, T.A.: The role of recombination in transfection of B. subtilis. Molec. gen. Genet. 113, 174–190 (1971)

    Google Scholar 

  • Trautner, T.A., Pawlek, B., Bron, S., Anagnostopoulos, C.: Restriction and modification in B. subtilis. Biological aspects. Molec. gen. Genet. 109, 181–191 (1974)

    Google Scholar 

Download references

Author information

Author notes

  1. K. J. Burger

    Present address: Institut für Mikrobiologie und Genetik, Universität Würzburg, Röntgenring 11, D-8700, Würzburg, Federal Republic of Germany

Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestrasse 63/73, D-1000, Berlin 33

    K. J. Burger & T. A. Trautner

Authors
  1. K. J. Burger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. A. Trautner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Experiments reported were part of the Doctoral Thesis submitted by K. Burger to the Freie Universität Berlin

Communicated by E. Bautz

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Burger, K.J., Trautner, T.A. Specific labelling of replicating SPP1 DNA. Molec. Gen. Genet. 166, 277–285 (1978). https://doi.org/10.1007/BF00267619

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267619

Keywords

  • Subtilis Strain
  • Specific Labelling
  • Nonpermissive Temperature
  • Initation Mutation
  • Bacteriophage SPP1