Skip to main content
SpringerLink
Log in

Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The genes coding for 4S RNA have been localized on the physical map of the chloroplast genome of Chlamydomonas reinhardii by hybridizing 32P-labelled 4S RNA to EcoRI, BamHI, BgIII and HindIII chloroplast DNA digests and to hybrid plasmids containing EcoRI and BamHI chloroplast DNA fragments. At least 10 EcoRI and 7 BamHI fragments carry sequences coding for 4S RNA. These genes are interspersed throughout the genome. The spacer between the 16S and 23S ribosomal RNA genes, which is repeated twice per chloroplast DNA molecule, codes for at least one 4S RNA, shown to be transcribed from the same strand as the ribosomal RNAs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allet, B.: Fragments produced by cleavage of λ deoxyribonucleic acid with the hemophilus parainfluenzae restriction enzyme HpaII. Biochemistry 12, 3972–3977 (1973)

    Google Scholar 

  • Bohnert, J.J., Driesel, A.J., Hermann, R.G.: Characterization of the RNA compounds synthesized by isolated chloroplasts. In: Genetics and biogenesis of chloroplasts and mitochondria (Th. Bücher et al., eds.) pp. 629–636. Amsterdam: Elsevier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Gorman, D.S., Levine, R.P.: Cytochrome F and plastocyanin: Their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardii. Proc. nat. Acad. Sci. (Wash.) 54, 1665–1669 (1965)

    Google Scholar 

  • Guillemaut, P., Weil, J.H.: Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial t-RNAsMet and of Escherichia coli t-RNAsMet by homologous and heterologous enzymes. Biochim. biophys. Acta (Amst.) 407, 240–248 (1975)

    Google Scholar 

  • Haff, L.A., Bogorad, L.: Hybridization of maize chloroplast DNA with transfer ribonuclei acids. Biochem. 15, 4105–4109 (1976)

    Google Scholar 

  • Hartley, M.R., Ellis, R.J.: Ribonucleic acid synthesis in chloroplasts. Biochem. J. 134, 249–262 (1978)

    Google Scholar 

  • Hoober, K.: Protein synthesis in chloroplasts. In: Protein synthesis, (McConkey, E.H., ed.), Vol. 2, pp. 169–248. Inc. New York: Marcel Dekker 1976

    Google Scholar 

  • Katz, L., Kingsbury, D.T., Helinski, D.R.: Stimulation by Cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J. Bact. 114, 577–591 (1973)

    Google Scholar 

  • Kislev, N., Selsky, M.J., Norton, C., Eisenstadt, J.M.: t-RNA and t-RNA aminoacylsynthetases of chloroplasts, mitochondria and cytoplasm from Euglena gracilis. Biochim. biophys. Acta (Amst.) 287, 256–269 (1972)

    Google Scholar 

  • Lund, E., Dahlberg, J.E., Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell 7, 165–177 (1976)

    Google Scholar 

  • Maxam, A.M., Gilbert, W.: A new method for sequencing DNA. Proc. nat. Acad. Sci. (Wash.) 74, 560–564 (1977)

    Google Scholar 

  • Ommen, G.J.B. van, Groot, G.S.P., Borst, P.: Fine structure physical mapping of 4S RNA gens on mitochondrial DNA of Saccharomyces cerevisiae. Molec. gen. Genet. 154, 255–262 (1977)

    Google Scholar 

  • Rochaix, J.D.: Restriction enzyme analysis of the chloroplast DNA of Chlamydomonas reinhardii and construction of chloroplast DNA-plasmid hybrids. In: Acides nucléiques et synthèse de protéines chez les Végétaux (Bogorad, L. and Weil, J.H., eds.) CNRS, pp. 77–83. Paris 1977

  • Rochaix, J.D.: Restriction fragments from Chlamydomas chloroplast DNA. In: Methods of enzymology, (Grossman, L. and Moldave, K., eds.). New York: Academic Press Inc. 1978

    Google Scholar 

  • Schwartzbach, S.D., Hecker, L.J., Barnett, W.E.: Transcriptional origin of Euglena chloroplast t-RNAs. Proc. nat. Acad. Sci. (Wash.) 73, 1984–1988 (1976)

    Google Scholar 

  • Southern, E.M.: Delection of specific sequences among DNA fragments separated by gel electrophoresis. J. molec. Biol. 98, 503–517 (1975)

    Google Scholar 

  • Wilson, G.A., Young, F.E.: Isolation of a sequence specific endonuclease (BamI) from Bacillus amylolique faciens H. J. molec. Biol. 97, 123–125 (1975)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, University of Geneva, CH-1211, Genève 4, Switzerland

    Pia Malnoë & Jean-David Rochaix

Authors
  1. Pia Malnoë
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean-David Rochaix
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gehring

Rights and permissions

Reprints and permissions

About this article

Cite this article

Malnoë, P., Rochaix, JD. Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii . Molec. Gen. Genet. 166, 269–275 (1978). https://doi.org/10.1007/BF00267618

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267618

Keywords

Search

Navigation