Skip to main content
SpringerLink
Log in

Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

When E. coli or λ infected E. coli are gently lysed the DNA is released as a very fast sedimenting species that is presumably bound to membrane material. If this complex is now subjected to restriction enzyme cleavage, only a minor fraction of the fast sedimenting DNA remains and this is found, after purification, to be enriched for branched molecules.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Gefter, M.L.: DNA replication. Annual review of biochemistry, Vol. 44, pp. 45–78 (E. Snell, ed.), Palornia: Annual Reviews, Inc. 1975

    Google Scholar 

  • Inman, R.B., Schnös, M.: Structure of branch points in replicating DNA: Presence of single-stranded connections in λ DNA branch points. J. molec. Biol. 56, 319–325 (1970)

    Google Scholar 

  • Parker, D.L., Glaser, D.A.: Effect of growth conditions on DNA-membrane attachment it Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 72, 2446–2450 (1975)

    Google Scholar 

  • Siegel, P.J., Schaechter, M.: The role of the host cell in the replication and morphogenesis of bacteriophages. Annual review of microbiology (M. Starr, ed.). Palo Alto, California: Annual Revies, Inc. 1973

    Google Scholar 

  • Tanaka, T., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: means of amplification of deoxyribonucleic acid. J. Bact. 121, 354–362 (1975)

    Google Scholar 

  • Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with EcoR1 restriction endonuclease. J. molec. Biol. 91, 315–328 (1975)

    Google Scholar 

  • Valenzuela, M.S.: Intermediates of the first round of λ DNA replication are preferentially found in a rapidly sedimenting complex. Biochem. biophys. Res. Commun. 65, 1221–1228 (1975)

    Google Scholar 

  • Valenzuela, M.S., Freifelder, D., Inman, R.B.: Lack of a unique termination site for the first round of bacteriophage λ DNA replication. J. molec. Biol. 102, 569–581 (1976)

    Google Scholar 

  • Valenzuela, M.S., Inman, R.B.: Visualization of a novel junction in bacteriophage λ DNA. Proc. nat. Acad Sci. (Wash.) 72, 3024–3028 (1975)

    Google Scholar 

  • Yamaguchi, K., Yoshikawa, H.: Chromosome-membrane association in Bacillus subtilis III. isolation and characterization of a DNA-protein complex carrying replication origin markers. J. molec. Biol. 110, 219–253 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biophysics Laboratory, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Manuel S. Valenzuela & Ross B. Inman

  2. Department of Biochemistry, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Manuel S. Valenzuela & Ross B. Inman

Authors
  1. Manuel S. Valenzuela
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ross B. Inman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by A.W. Boyer

Rights and permissions

Reprints and permissions

About this article

Cite this article

Valenzuela, M.S., Inman, R.B. Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli . Molec. Gen. Genet. 166, 245–249 (1978). https://doi.org/10.1007/BF00267615

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267615

Keywords

Search

Navigation