Skip to main content
SpringerLink
Log in

The genome of B. subtilis phage SPP1: Assignment of 5′–3′-polarity to the complementary strands of SPP1 DNA

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The left part of the SPP1 chromosome can be defined by the presence of dispensable DNA. With this definition, the polarities of the separable H and L strands of SPP1 DNA are 5′–3′ and 3′–5′, respectively, from left to right.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atkinson, M.R., Deutscher, M.P., Kornberg, A., Russel, A.F., Mofatt, J.G.: Enzymatic synthesis of deoxyribonucleic acid. XXXIV Termination of chain growth by a 2′, 3′-dideoxyribonucleotide. Biochemistry 8, 4897–4904 (1969)

    Google Scholar 

  • Blin, N., Gabain, A.V., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Letters 53, 84–86 (1975)

    Google Scholar 

  • Esche, H., Spatz, H.Ch.: Asymmetric transcription of SPP1 in vivo. Molec. gen. Genet. 124, 57–63 (1973)

    Google Scholar 

  • Greene, P.J., Betlack, M., Goodman, H.M., Boyer, H.: DNA replication and biosynthesis. Methods molec Biol. 7, 87–111 (1974)

    Google Scholar 

  • Ikeda, H., Tomizawa, J.-I.: Prophage P1, an extrachromosomal replication unit. Cold Spr. Harb. Symp. quant. Biol., 33, 791–798 (1968)

    Google Scholar 

  • Morris, Ch. F., Sinha, N.K., Alberts, B.M.: Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: Rolling circle replication following de novo chain initiation on a single-stranded circular DNA template. Proc. nat. Acad. Sci. (Wash.) 72, 4800–4804 (1975)

    Google Scholar 

  • Riva, S.C.: Asymmetric transcription of B. subtilis phage SPP1 DNA in vitro. Biochem. biophys. Res Commun. 34, 824–830 (1969)

    Google Scholar 

  • Rhoades, M., MacHattie, L.A., Thomas, Jr., C.A.: The P22 bacteriophage DNA molecule. I. The mature form. J. molec. Biol. 37, 21–40 (1968)

    Google Scholar 

  • Russell, A.F., Moffatt, J.G.: Synthesis of some nucleotides derived from 3′-deoxythymidine. Biochemistry 8, 4889–4896 (1969)

    Google Scholar 

  • Scherzinger E., Seiffert, D.: Studies on bacteriophage T7 DNA synthesis in vitro. I. Resolution of the T7 replication system into its components. Molec. gen. Genet. 141, 213–232 (1975)

    Google Scholar 

  • Smith, H.O., Birnstiel, M.L.: A simple method for DNA restriction site mapping. Nucleic Acids Res. 3, 2387–2398 (1976)

    Google Scholar 

  • Spatz, H.Ch., Trautner, T.A.: One way to do experiments on gene conversion? Molec. gen. Genet. 109, 84–106 (1970)

    Google Scholar 

  • Trautner, T.A., Spatz, H.Ch., Berhrens, B., Pawlek, B., Behncke M.: Exchange between complementary strands of DNA? Adv. in Biosc. 8, 79–87 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestraße 63/73, D-1000, Berlin 33

    G. Morelli, M. A. Montenegro & T. A. Trautner

  2. Abt. Schuster, Max-Planck-Institut für molekulare Genetik, Ihnestraße 63/73, D-1000, Berlin 33

    G. Hillenbrandt & E. Scherzinger

Authors
  1. G. Morelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Montenegro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Hillenbrandt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Scherzinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. A. Trautner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by E. Bautz

Part of this work was taken from the doctoral thesis of M.A.M., which is to be submitted to the Freie Universität, Berlin

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morelli, G., Montenegro, M.A., Hillenbrandt, G. et al. The genome of B. subtilis phage SPP1: Assignment of 5′–3′-polarity to the complementary strands of SPP1 DNA. Molec. Gen. Genet. 164, 93–97 (1978). https://doi.org/10.1007/BF00267603

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267603

Keywords

Search

Navigation