Summary
Transcription of Escherichia coli ribosomal DNA introduced into Proteus mirabilis on F14 is described. We have developed an assay for E. coli coded ribosomal RNA involving fingerprinting of ribonuclease T1 digests of RNA isolated from ribosomal subunits. Sequence differences in the ribosomal RNA of the two species have allowed us to detect E. coli coded 16S, 23S, and 5S ribosomal RNA in ribosomal subunits of the E. coli-P. mirabilis hybrid. The proportion of E. coli coded rRNA in the hybrid is found at a level which is compatible with the number of E. coli (and P. mirabilis) ribosomal DNA sequences. The resulting ribosomal RNA appears in ribosomes in a form which indicates extensive compatibility of E. coli coded ribosomal RNA with P. mirabilis ribosomal proteins and maturational factors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baumberg, S., Dennison, S.: Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli. J. Bact. 123, 178–286 (1975)
Birnbaum, L.S., Kaplan, S.: Localization of a portion of the ribosomal RNA genes in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 925–929 (1971)
Brownlee, G.G., Cartwright, E.: Sequence studies on precursor 16S ribosomal RNA of Escherichia coli. Nature (Lond.) New Biol. 232, 50–52 (1971)
Colby, C., Jr., Martin, F.D., Hu, A.S.L.: Catabolite repression of the synthesis of β-galactosidase in Proteus mirabilis F-lac. Biochim. biophys. Acta (Amst.) 157, 159–166 (1968)
Colby, C., Jr., Hu, A.S.L.: The regulation of the synthesis of β-galactosidase in Proteus mirabilis F-lac. Biochim. biophys. Acta (Amst.) 157, 149–158 (1968)
Dale, J.W., Smith, J.T.: The purification and properties of the β-Lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis. Biochem. J. 123, 493–500 (1971)
Deonier, R.C., Ohtsubo, E., Lee, H.J., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli, VII. Mapping the ribosomal RNA genes of plasmid F14. J. molec. Biol. 89, 619–629 (1974)
Falkow, D., Wohlhieter, J.A., Citarella, R.V., Baron, L.S.: Transfer of episomic elements to Proteus. I. Transfer of F-linked chromosomal determinants. J. Bact. 87, 209–219 (1964)
Fellner, P., Ehresmann, C., Ebel, J.P.: Nucleotide sequences present in the 16S ribosomal RNA of Escherichia coli. Nature (Lond.) 225, 26–29 (1970)
Fellner, P., Ehresmann, C., Ebel, J.P.: The determination of the primary structure of 16S ribosomal RNA of Escherichia coli. (1) Nucleotide sequence analysis of T1 and pancreatic ribonuclease digestion products. Biochemie 54, 853–900 (1972)
Forget, B.G., Jordan, B.: 5S RNA synthesized by Escherichia coli in presence of chloramphenicol: different 5′-terminal sequences. Science 167, 382–384 (1969)
Fox, G.E., Woese, C.R.: 5S RNA secondary structure. Nature (Lond.) 256, 505–507 (1975)
Franklin, T.J., Rownd, R.: R-Factor-mediated resistance to tetracycline in Proteus mirabilis. J. Bact. 115, 235–242 (1973)
Ginsburg, D., Seitz, J.A.: The 30S ribosomal precursor RNA from Escherichia coli. A primary transcript containing 23S, 16S and 5S sequences. J. biol. Chem. 250, 5647–5654 (1975)
Hayes, F., Hayes, D., Fellner, P., Ehresmann, C.: Additional nucleotide sequences in precursor 16S ribosomal RNA from Escherichia coli. Nature (Lond.) New Biol. 232, 54–55 (1971)
Jarry, B., Rosset, R.: Heterogeneity of 5S RNA cn Escherichia coli. Molec. gen. Genet. 113, 43–50 (1971)
Jarry, B., Rosset, R.: Further mapping of 5S RNA cistrons in Escherichia coli. Molec. gen. Genet. 126, 29–35 (1973)
Kim, S.H., Sussman, J.L., Suddath, F.L., Quigley, G.J., McPherson, A., Wang, A.H.J., Seeman, N.C., Rich, A.: The general structure of transfer RNA molecules. Proc. nat. Acad. Sci. (Wash.) 71, 4970–4974 (1974)
Kontomichalou, P.: Transmissable extrachromosomal resistance to the penicillins in Escherichia coli K12 and Falkow's Proteus host. 5th. Int. Congr. Chemotherapy 4, 251–256 (1967)
Low, K.B.: Escherichia coli K-12 F-prime factors, old and new. Bact. Rev. 36, 587–607 (1972)
Lowry, C.U., Dahlberg, J.E.: Structural differences between the 16S ribosomal RNA of E. coli and its precursor. Nature (Lond.) New Biol. 232, 52–54 (1971)
Maaløe, O., Hanawalt, P.: Thymine deficiency and the normal DNA replication cycle. I. J. molec. Biol. 3, 144–155 (1961)
Nashimoto, H., Nomura, M.: Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneously assembly of 30S subunits. Proc. nat. Acad. Sci. (Wash.) 67, 1440–1447 (1970)
Nikolaev, N., Glazier, D., Schlessinger, D.: Cleavage by Ribonuclease III of the complex of 30S pre-ribosomal and ribosomal proteins of Escherichia coli. J. molec. Biol. 94, 301–304 (1975)
Okamota, S., Suzuke, Y., Mise, K., Nakaya, R.: Occurrence of chloramphenicol acetylating enzymes in various gram-negative bacilli. J. Bact. 94, 1616–1622 (1967)
Sangerl, F., Brownlee, G.G.: A two-dimensional fractionation method for radioactive nucleotides. Methods in Enzymology 12, 361–381 (1967)
Schlessinger, D.: In: Ribosomes (ed. Nomura, M., Tissières, A., Lengyel, P.). Cold Spring Harbor Laboratory (1974)
Shine, J., Dalgarno, L.: The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. nat. Acad. Sci. (Wash.) 71, 1342–1346 (1974)
Smith, J.T.: R-factor gene expression in gram-negative bacteria. J. gen. Microbiol. 55, 109–120 (1969)
Sogin, M., Pace, B., Pace, N.R., Woese, C.R.: Primary structural relationship of p16 to m16 ribosomal RNA. Nature (Lond.) New Biol. 232, 48–49 (1971)
Sogin, M.L., Pechman, K.J., Zablen, L., Lewis, B.J., Woese, C.R.: Observations on the post-transcriptionally modified nucleotides in the 16S ribosomal ribonucleic acid. J. Bact. 112, 13–16 (1972)
Sogin, S.J., Sogin, M.L., Woese, C.R.: Phylogenetic measurement in procaryotes by primary structural characterization. J. molec. Evol. 1, 173–184 (1972)
Stubbs, J., Horwitz, A., Moses, V.: Studies on β-galactoside transport in a Proteus mirabilis merodiploid carrying an Escherichia coli lactose operon. J. Bact. 116, 131–140 (1973)
Uchida, T., Bonen, L., Schaup, H., Lewis, B., Zablen, L., Woese, C.R.: The use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of E. coli 16S ribosomal RNA. J. molec. Evol. 3, 63–67 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Morgan, E.A., Kaplan, S. Transcription of Escherichia coli ribosomal DNA in Proteus mirabilis . Molec. Gen. Genet. 147, 179–188 (1976). https://doi.org/10.1007/BF00267569
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267569