Summary
Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnett, W.E., Pennington, C.J., Jr., Fairfield, S.A.: Induction of euglena transfer RNA's by light. Proc. nat. Acad. Sci. (Wash.) 63, 1261–1268 (1969)
Chua, N.-H., Blobel, G., Siekevitz, P.: Isolation of cytoplasmic and chloroplast ribosomes and their dissociation into active subunits from Chamydomonas reinhardti. J. Cell Biol. 57, 798–814 (1973)
Cross, J.W., McMahon, D.: Chloral hydrate causes breakdown of polysomes in Chlamydomonas reinhardi. J. biol. Chem. 251, 2637–2643 (1976)
Evins, W.H.: Enhancement of polyribosome formation and induction of tryptophan-rich proteins by gibberellic acid. Biochem. 10, 4295–4303 (1971)
Fangman, W.L., Neidhardt, F.C.: Protein and ribonucleic acid synthesis in a mutant of Escherichia coli with a altered amino-acyl synthetase. J. biol. Chem. 239, 1844–1847 (1964)
Fleck, A., Munro, H.N.: The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim. biophys. Acta (Amst.) 55, 571–583 (1962)
Folk, W.R., Berg, P.: Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo J. Bact. 102, 204–212 (1970)
Givan, A.L., Criddle, R.S.: Ribulose-diphosphate carboxylase from Chlamydomonas reinhardi: Purification, properties and its mode of synthesis in the cell. Arch. Biochem. biophys. 149, 153–163 (1972)
Hartwell, L., McLaughlin, C.S.: Mutants of yeast with temperature-sensitive isoleucyl tRNA synthetases. Proc. nat. Acad. Sci. (Wash.) 59, 422–428 (1968)
Hartwell, L.H., McLaughlin, C.S.: A mutant of yeast apparently defective in the initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 62, 469–474 (1969)
Holley, R.W., Apgar, J., Doctor, B.P., Farrow, J., Marini, M.A., Merril, S.: A simplified procedure for the preparation of tyrosine-and valine-acceptor fractions of yeast “soluble ribonucleic acid”. J. biol. Chem. 236, 200–202 (1961)
Kaplan, S., Anderson, D.: Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J. Bact. 95, 991–997 (1968)
Lee-Ping, W.W.-J.: Studies on arginyl tRNA and arginyl tRNA synthetase during differentiation in Chlamydomonas reinhardi. M.A. Thesis, State. Univ. of New York, Stony Brook (1970)
Lodish, H.F.: Alpha and beta globin messenger ribonucleic acid. J. biol. Chem. 246, 7131–7138 (1971)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mans, R.J., Novelli, G.D.: Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method. Arch. Biochem. Biophys. 94, 48–53 (1961)
Martin, T.E.: A simple general method to determine the proportion of active ribosomes in eukaryotic cells. Exp. Cell Res. 80, 496–498 (1973)
MacLaughlin, C.S., Hartwell, L.H.: A mutant of yeast with a defective methionyl tRNA synthetase. Genetics 61, 557–566 (1969)
McLaughlin, C.S., Magee, P.T., Hartwell, L.H.: Role of isolencyltransfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression in yeast. J. Bact. 100, 579–584 (1969)
McMahon, D.: The isolation of mutants conditionally defective in protein synthesis in Chlamydomonas reinhardi. Molec. gen. Genet. 112, 80–86 (1971)
McMahon, D.: Cycloheximide is not a specific inhibitor of protein synthesis in vivo. Plant Physiol. 55, 815–821 (1975)
McMahon, D., Blaschko, W.: Chloral hydrate inhibits protein synthesis in vivo. Biochim. biophys. Acta (Amst.) 238, 338–342 (1971)
McMahon, D., Langstroth, P.: The effects of canavanine and of arginine starvation on macromolecular synthesis in Chlamydomonas reinhardi. J. gen Microbiol. 73, 239–250 (1972)
Menninger, J.R., Walker, C., Tan, P.F., Atherly, A.G.: Studies on the metabolic role of peptidyl-tRNA hydrolase. Molec. gen. Genet. 121, 307–324 (1973)
Miller, M.J., McMahon, D.: Synthesis and maturation of chloroplast and cytoplasmic ribosomal RNA in Chlamydomonas reinhardi. Biochim. biophys. Acta (Amst.) 366, 35–44 (1974)
Nomura, Y.: Irreversible inactivation at high temperature of temperature-sensitive mutant tRNATYR in vivo. Nature (Lond.) New Biol. 242, 12–14 (1973)
Petersen, N.S., McLaughlin, C.S.: Polysome metabolism in protein synthesis mutants of yeast. Molec. gen. Genet. 129, 189–200 (1974)
Schmidt, G., Thannhauser, J.: A method for the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissue. J. biol. Chem. 161, 83–89 (1945)
Thompson, L.H., Harkins, J.L., Stanners, C.P.: A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase. Proc. nat. Acad. Sci. (Wash.). 70, 3094–3098 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Cross, J., McMahon, D. Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii . Molec. Gen. Genet. 147, 169–177 (1976). https://doi.org/10.1007/BF00267568
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00267568