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A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis

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Summary

A new genetic nomenclature for the macromolecules involved in bacterial protein synthesis is proposed and explained. Genes for ribosomal proteins are designated rsp, rpl and rpm while genes for ribosomal RNAs are rrs and rrl. Protein synthesis factors and ribosome assembly and modification activities are also consistantly named.

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References

  • Apirion, D., Watson, N.: Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid. J. Bact. 124, 317–324 (1975)

    Google Scholar 

  • Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)

    Google Scholar 

  • Bernadi, A., Leder, P.: Protein biosynthesis in Escherichia coli. Purification and characteristics of a mutant G factor. J. biol. Chem. 245, 4263–4268 (1970)

    Google Scholar 

  • Birge, E.A., Kurland, C.G.: Altered ribosomal protein in streptomycin-dependent Escherichia coli. Science 166, 1282–1286 (1969)

    Google Scholar 

  • Birge, E.A., Kurland, C.G.: Reversion of a streptomycin-dependent strain of Escherichia coli. Molec. gen. Genet. 109, 356–369 (1970)

    Google Scholar 

  • Birge, E.A., Craven, G.R., Hardy, S.J.S., Kurland, C.G., Voynow, P.: Structure determinant of a ribosomal protein: K locus. Science 164, 1285–1286 (1969)

    Google Scholar 

  • Birnbaum, L.S., Kaplan, S.: Localization of a portion of the ribosomal RNA genes in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 925–929 (1971)

    Google Scholar 

  • Böck, A., Ruffler, D., Piepersberg, W., Wittmann, H.G.: Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of E. coli. Molec. gen. Genet 134, 325–332 (1974)

    Google Scholar 

  • Bollen, A., Davies, J., Ozaki, M., Mizushima, M.: Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli. Science 165, 85–86 (1969)

    Google Scholar 

  • Bollen, A., Faelen, M., Lococq, J.P., Herzog, A., Zengel, J., Kahan, L., Nomura, M.: The structural gene for the ribosomal protein S18 in Escherichia coli. I. Genetic studies on a mutant having an alteration in the protein S18. J. molec. Biol. 76, 463–472 (1973)

    Google Scholar 

  • Breckenridge, L., Gorini, L.: Genetic analysis of streptomycin resistance in Escherichia coli. Genetics 65, 9–25 (1970)

    Google Scholar 

  • Brot, N., Weisbach, H.: The enzymatic acetylation of E. coli ribosomal protein L12. Biochem. biophys. Res. Commun. 49, 673–679 (1972)

    Google Scholar 

  • Brown, M.E., Apirion, D.: Mapping a cluster of ribosomal genes in E. coli. Molec. gen. Genet. 133, 317–327 (1974)

    Google Scholar 

  • Bryant, R.E., Sypherd, P.S.: Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli. J. Bact. 117, 1082–1092 (1974)

    Google Scholar 

  • Cannon, M., Cabezon, T., Bollen, A.: Mapping of neamine resistance: Indentification of two genetic loci, neaA and neaB. Molec. gen. Genet. 130, 321–326 (1974)

    Google Scholar 

  • Caskey, T., Leder, P., Moldave, K., Schlessinger, D.: Translation: its mechanism and control. Science 176, 195–197 (1972)

    Google Scholar 

  • Chang, C.N., Chang, F.N.: Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S proteins. Biochemistry 14, 468–477 (1975)

    Google Scholar 

  • Demerec, M., Adelberg, E.A., Clark, A.J., Hartman, P.E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966)

    Google Scholar 

  • Deonier, R.C., Ohtsubo, E., Lee, H.J., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. VII. Mapping the ribosomal RNA genes of plasmid F14. J. molec. Biol. 89, 619–629 (1974)

    Google Scholar 

  • Deusser, E., Stöffler, G., Wittmann, H.G., Apirion, D.: Ribosomal proteins. XVI. Altered S4 proteins in Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 109, 298–302 (1970)

    Google Scholar 

  • DeWilde, M., Michel, F., Broman, K.: The structural gene for ribosomal protein S18 in Escherichia coli. III. Mapping outside the ribosomal protein gene cluster at minute 84 on the genome. Molec. gen. Genet. 133, 329–333 (1974)

    Google Scholar 

  • Dunn, J.J., Studier, F.W.: T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease III. Proc. nat. Acad. Sci. (Wash.) 70, 3296–3300 (1973)

    Google Scholar 

  • Fellner, P.: Structure of the 16S and 23S ribosomal RNAs. In: Ribosomes (M. Nomura, A. Tissieres, and P. Lengyel, eds.), pp. 169–191. Cold Spring Harbor Laboratory (1974)

  • Friesen, J.D., Parker, J., Watson, R.J., Fiil, N.P., Pedersen, S.: Isolation of a transducing phage carrying rpsT, the structural gene for ribosomal protein S20. Molec. gen. Genet. 144, 115–118 (1976)

    Google Scholar 

  • Funatsu, G., Puls, W., Schlitz, E., Reinbolt, J., Wittmann, H.G.: Ribosomal proteins. XXXI. Comparative studies on altered proteins S4 of six Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 115, 131–139 (1972)

    Google Scholar 

  • Garvin, R.T., Gorini, L.: A new gene for ribosomal restriction in Escherichia coli. Molec. gen. Genet. 137, 73–78 (1975)

    Google Scholar 

  • Ginsburg, D., Steitz, J.A.: The 30S ribosomal precursor RNA from Escherichia coli. J. biol. Chem. 250, 5647–5654 (1975)

    Google Scholar 

  • Guthrie, C., Nashimoto, H., Nomura, M.: Structure and function of Escherichia coli ribosomes. VIII. Cold-sensitive mutants defective in ribosome assembly. Proc. nat. Acad. Sci. (Wash.) 63, 384–391 (1969a)

    Google Scholar 

  • Guthrie, C., Nashimoto, H., Nomura, M.: Studies on the assembly of ribosomes in vivo. Cold Spring Harb. Symp. quant. Biol. 34, 69–75 (1969b)

    Google Scholar 

  • Haselkorn, R., Rothman-Denes, L.B.: Protein synthesis. Ann. Rev. Biochem. 42, 397–438 (1973)

    Google Scholar 

  • Hasenbank, R., Guthrie, C., Stöffler, G., Wittmann, H.G., Rosen, L., Apirion, D.: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 127, 1–18 (1973)

    Google Scholar 

  • Helser, T.L., Davies, J.E., Dahlberg, J.E.: Mechanism of kasugamycin resistance in Escherichia coli. Nature (Lond.) New Biology 235, 6–9 (1972)

    Google Scholar 

  • Jarry, B., Rosset, R.: Further mapping of 5S RNA cistrons in Escherichia coli. Molec. gen. Genet. 126, 29–35 (1973)

    Google Scholar 

  • Jaskunas, S.R., Nomura, M., Davies, J.: Genetics of bacterial ribosomes. In: Ribosomes (M. Nomura, A. Tissieres, P. Lengyel, eds.), p. 333–368. Cold Spring Harbor Laboratory (1974)

  • Jaskunas, S.R., Lindahl, L., Nomura, M.: Specialized transducing phages for ribosomal protein genes of E. coli. Proc. nat. Acad. Sci. (Wash.) 72, 2743–2747 (1975a)

    Google Scholar 

  • Jaskunas, S.R., Lindahl, L., Nomura, M., Burgess, R.R.: Identification of two copies of the gene for the elongation factor EF-Tu in Escherichia coli. Nature (Lond.) 257, 458–462 (1975b)

    Google Scholar 

  • Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc. nat. Acad. Sci. (Wash.) 67, 1276–1282 (1970)

    Google Scholar 

  • Kang, S.-S.: A mutant of Escherichia coli with temperature-sensitive streptomycin protein. Proc. nat. Acad. Sci. (Wash.) 65, 544–550 (1970)

    Google Scholar 

  • Kreider, G., Brownstein, B.L.: Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli. J. Bact. 109, 780–783 (1972)

    Google Scholar 

  • Kushner, S.R., Maples, V.F., Champney, W.S.: Conditionally lethal ribosomal protein mutants: Characterization of a locus required for modification of 50S subunit proteins. Proc. nat. Acad. Sci. (Wash.) submitted

  • Kuwano, M., Schlessinger, D., Rinaldi, G., Felicetti, L., Tochini-Valentini, G.P.: G factor mutants of Escherichia coli: Map location and properties. Biochem. biophys. Commun. 42, 441–444 (1971)

    Google Scholar 

  • Kuwano, M., Ono, M., Yamamato, M., Endo, H., Kamiya, T., Hori, K.: Elongation factor T altered in a temperature-sensitive Escherichia coli mutant. Nature (Lond.) New Biol. 244, 107–109 (1973)

    Google Scholar 

  • Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes in E. coli for ribosomal proteins, ribosomal RNA and RNA polymerase subunits. Proc. nat. Acad. Sci. (Wash.) 72, 2743–2747 (1975)

    Google Scholar 

  • Matsubara, M., Takata, R., Osawa, S.: Chromosomal loci for 16S ribosomal RNA in Escherichia coli. Molec. gen. Genet. 117, 311–317 (1972)

    Google Scholar 

  • Mayuga, C., Meier, D., Wang, T.: Escherichia coli: The K12 ribosomal protein and the streptomycin region of the chromosome. Biochem. biophys. Res. Commun. 33, 203–206 (1968)

    Google Scholar 

  • Momose, H., Gorini, L.: Genetic analysis of streptomycin dependence in Escherichia coli. Genetics 67, 19–38 (1971)

    Google Scholar 

  • Nashimoto, H., Uchida, H.: Late steps in the assembly of 30S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli. J. molec. Biol. 96, 443–453 (1975)

    Google Scholar 

  • Nikolaev, N., Silengo, L., Schlessinger, D.: Synthesis of a large precursor to ribosomal RNA in a mutant of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 70, 3361–3365 (1973)

    Google Scholar 

  • Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature (Lond.) 222, 333–339 (1969)

    Google Scholar 

  • Pace, B., Pace, N.R.: Gene dosage for 5S ribosomal RNA in Escherichia coli and Bacillus megaterium. J. Bact. 105, 142–149 (1971)

    Google Scholar 

  • Parker, J., Watson, R.J., Friesen, J.D., Fiil, N.P.: A relaxed mutant with an altered ribosomal protein L11. Molec. gen. Genet. 144, 111–114 (1976)

    Google Scholar 

  • Reiner, A.M.: Genetic locus for ribonuclease I in Escherichia coli. J. Bact. 97, 1522–1523 (1969)

    Google Scholar 

  • Rosset, R., Vola, C., Feunteun, J., Monier, R.: A thermosensitive mutant defective in ribosomal 30S subunit assembly. FEBS Letters 18, 127–129 (1971)

    Google Scholar 

  • Sparling, P.F.: Kasugamycin resistance: A 30S ribosomal mutatiori with an unusual location on the Escherichia coli chromosome. Science 167, 56–58 (1968)

    Google Scholar 

  • Stöffler, G., Deusser, E., Wittmann, H.G.: Ribosomal proteins: XIX. Altered S5 ribosomal protein in an Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 111, 334–341 (1971)

    Google Scholar 

  • Studier, F.W.: Genetic mapping of a mutation that causes ribonuclease III deficiency in Escherichia coli. J. Bact. 124, 307–316 (1975)

    Google Scholar 

  • Sypherd, P.S., Osawa, S.: Ribosome genetics revealed by hybrid bacteria. In: Ribosomes (M. Nomura, A. Tissieres, P. Lengyel, eds.), pp. 669–678. Cold Spring Harbor Laboratory (1974)

  • Tanaka, N., Kawano, G., Kinoshita, T.: Chromosomal location of a fusidic acid resistant marker in Escherichia coli. Biochem. biophys. Res. Commun. 42, 564–567 (1971)

    Google Scholar 

  • Terhorst, C., Möller, W., Laursen, R., Wittmann-Liebold, B.: Amino acid sequence of a 50S ribosomal protein involved in both EFG and EFT dependent GTP-hydrolysis. FEBS Letters 28, 325–328 (1972)

    Google Scholar 

  • Tocchini-Valentini, G.P., Mattoccia, E.: A mutant of E. coli with an altered supernatant factor. Proc. nat. Acad. Sci. (Wash.) 61, 146–151 (1968)

    Google Scholar 

  • Turnock, G.: A genetic analysis of a mutant of Escherichia coli with a defect in the assembly of ribosomes. Molec. gen. Genet. 104, 295–312 (1969)

    Google Scholar 

  • Unger, M., Birnbaum, L.S., Kaplan, S., Pfister, A.: Location of the ribosomal RNA cistroms of E. coli: A second site. Molec. gen. Genet. 119, 377–380 (1972)

    Google Scholar 

  • Watson, R.J., Parker, J., Fiil, N.P., Flasks, J.G., Friesen, J.D.: New chromosomal location for structural genes of ribosomal proteins. Proc. nat. Acad. Sci. (Wash.) 72, 2765–2769 (1975)

    Google Scholar 

  • Wittmann, H.G.: Purification and identification of Escherichia coli ribosomal proteins. In: Ribosomes (M. Nomura, A Tissieres, P. Lengyel, eds.), pp. 93–114. Cold Spring Harbor Laboratory (1974)

  • Wittmann, H.G., Stöffler, G., Hindennach, I., Kurland, C.G., Randall-Hazelbauer, L., Birge, E.A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R.R., Bickle, T.A.: Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Molec. gen. Genet. 111, 327–333 (1971)

    Google Scholar 

  • Wittmann, H.G., Stöffler, G., Apirion, D., Rosen, L., Tanaka, K., Tamaki, M., Tanaka, R., Dekio, S., Otaka, E., Osawa, S.: Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins. Molec. gen. Genet. 127, 175–189 (1973)

    Google Scholar 

  • Wittmann, H.G., Stöffler, G., Geyl, D., Böck, A.: Alterations of ribosomal proteins in revertants of a valyl-t TNA synthetase mutant of Escherichia coli. Molec. gen. Genet. 141, 317–329 (1975)

    Google Scholar 

  • Wright, M.: Mutants of Escherichia coli lacking endonuclease I, ribunuclease I, or ribonuclease II. J. Bact. 107, 87–94 (1971)

    Google Scholar 

  • Wu, M., Davidson, N.: Use of gene 32 protein staining of single-strand polynucleotides for gene mapping by electron microscopy: Applications to the ϕ80d3 ilvsu + 7 system. Proc. nat. Acad. Sci. (Wash.) 72, 4506–4510 (1975)

    Google Scholar 

  • Yoshikawa, M., Okuyama, A., Tanaka, N.: A third kasugamycin resistance locus ksgC, affecting ribosomal protein S2 in Escherichia coli K-12. J. Bact. 122, 796–797 (1975)

    Google Scholar 

  • Yoshikawa, M., Okuyama, A., Tanaka, N.: A third kasugamycin resistance locus ksgC, affecting ribosomal protein S2 in Escherichia coli K-12. J. Bact. 122, 796–797 (1975)

    Google Scholar 

  • Zimmermann, R.A., Garvin, R.T., Gorini, L.: Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 2263–2267 (1971)

    Google Scholar 

  • Zimmermann, R.A., Ikeya, Y., Sparling, P.F.: Alteration of ribosomal protein S4 by mutation linked to kasugamycin resistance in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 70, 71–75 (1973)

    Google Scholar 

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  1. Program in Genetics, Department of Biochemistry, University of Georgia, 30602, Athens, Georgia, USA

    W. Scott Champney & Sidney R. Kushner

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  1. W. Scott Champney
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  2. Sidney R. Kushner
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Communicated by H.G. Wittmann

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Champney, W.S., Kushner, S.R. A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis. Molec. Gen. Genet. 147, 145–151 (1976). https://doi.org/10.1007/BF00267566

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