Summary
In Aspergillus nidulans expression of the gabA gene, the probable structural gene for the γ-amino-n-butyrate (GABA) permease, is controlled by induction, via the intA gene, ammonium repression, mediated by the areA gene, and probably carbon catabolite repression. Regulatory mutations, tightly linked to gabA, were selected by reverting an areA-2 strain on GABA as nitrogen source. These mutations, gabI-1, gabI-2, and gabI-3 result in increased gabA expression and are cis-dominant in their effects on the gabA gene. Mapping data show that the regulatory mutations map on one side of all gabA- alleles tested.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alderson, T., Hartley, M.J.: Specificity for spontaneous and induced forward mutation at several gene loci in Aspergillus nidulans. Mutation Res. 8, 255–264 (1969).
Arst, H.N., Jr.: Intergrator gene in Aspergillus nidulans. Nature (Lond.). 262, 231–234 (1976)
Arst, H.N., Jr.: Some genetical aspects of ornithine metabolism in Aspergillus nidulans. Molec. gen. Genet. 151, 105–110 (1977)
Arst, H.N., Jr.: GABA transaminase provides an alternative route of β-alanine synthesis in Aspergillus nidulans. Molec. gen. Genet. 163,23–27 (1978)
Arst, H. N., Jr., Bailey, C.R.: The regulation of carbon metabolism in Aspergillus nidulans. In: Genetics and physiology of Aspergillus (J.E. Smith and J.A. Pateman, eds.) pp. 131–146. London: Academic Press 1977
Arst, H.N., Jr., Cove, D.J.: Nitrogen metabolite repression in Aspergillus nidulans. Molec. gen. Genet. 126, 111–141 (1973)
Arst, H.N., Jr., MacDonald, D.W.: A gene cluster in Aspergilus nidulans with an internally located cis-acting regulatory region. Nature (Lond.) 254, 26–31 (1975).
Arst, H.N., Jr., Penfold, H.A., Bailey, C.R.: Lactam utilisation in Aspergillus nidulans: evidence for a fourth gene under the control of the integrator gene intA. Molec. gen. Genet. 166, 321–327 (1978)
Arst, H.N., Jr., Scazzocchio, C.: Initiator constitutive mutation with an ‘up-promoter’ effect in Aspergillus nidulans. Nature (Lond.) 254, 31–34 (1975)
Bailey, C.R.: Carbon catabolite repression in Aspergillus nidulans. Ph. D. Thesis, University of Cambridge (1976)
Bailey, C.R., Arst, H.N., Jr.: Carbon catabolite repression in Aspergillus nidulans. Eur. J. Biochem. 51, 573–577 (1975)
Bartnik, E., Weglenski, P.: Regulation of arginine catabolism in Aspergillus nidulans. Nature (Lond.) 250, 590–592 (1974)
Clutterbuck, A. J.: Aspergillus nidulans. In: Handbook of genetics, Vol. 1, pp. 447–510 (R.C. King, ed.). New York: Plenum Press 1974
Cove, D.J.: The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 113, 51–56 (1966)
Herman, C., Clutterbuck, A.J.: A method for the selection of auxotrophs by means of ‘spidery’ growth. Aspergillus News Letter 7, 13–14 (1966)
Hynes, M.J.: A cis-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus midulans. Nature (Lond.) 253, 210–212 (1975)
Lukaszkiewicz, Z., Paszewski, A.: Hyper-repressible operator-type mutant in sulphate permease gene of Aspergillus nidulans. Nature (Lond.) 259, 337–338 (1976).
Kinghorn, J.R., Pateman, J.A.: Studies of partially repressed mutants at the tamA and areA loci in Aspergillus nidulans. Molec. gen. Genet. 140, 137–147 (1975)
Penfold, H.A.: ω-amino acid catabolism in Aspergillus nidulans. Ph. D. Thesis, University of Cambridge (1978)
Penfold, H.A., Bailey, C.R., Arst, H.N., Jr.: An integrator gene in Aspergillus nidulans: regulatory and metabolic roles of ω-amino acids. Heredity 39, 433 (1977)
Pontecorvo, G., Roper, J.A., Hemmons, L.M., Macdonald, K.D., Bufton, A.W.J.: The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238 (1953)
Rand, K.N., Arst, H.N., Jr.: A mutation in Aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene. Molec. gen. Genet. 155, 67–75 (1977)
Author information
Authors and Affiliations
Additional information
Communicated by W. Gajewski
Rights and permissions
About this article
Cite this article
Bailey, C.R., Penfold, H.A. & Arst, H.N. Cis-dominant regulatory mutations affecting the expression of GABA permease in Aspergllus nidulans . Molec. Gen. Genet. 169, 79–83 (1979). https://doi.org/10.1007/BF00267548
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267548