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Comparative studies on mitochondrial development in yeasts

II. Mitochondrial ribosomes from Saccharomyces cerevisiae

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Summary

High molecular weight mitochondrial RNA from Saccharomyces cerevisiae can be isolated rapidly and in relatively high yield from mitochondria prepared from cells prefixed with glutaraldehyde and disrupted mechanically. The RNA has lower electrophoretic mobilities than corresponding species from cytoplasmic ribosomes, and can also be distinguished from cytoplasmic RNA on the basis of the sensitivity of the mobility to temperature. RNA from cytoplasmic ribosomes and mitochondria of Candida parapsilosis shows a similar differential response to temperature.

Mitochondrial ribosomes in Saccharomyces cerevisiae do not appear to be distinguishable from the cytoplasmic particles on the basis of sedimentation velocity. They can be identified, however, by pulse-labelling cells in the presence of cycloheximide. Cytoplasmic ribosomes under these conditions do not label. The labelling of mitochondrial ribosomes is sensitive to chloramphenicol, and is dispersed over the polysomal or ribosomal aggregate region of density gradients.

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References

  • Ashwell, M. A., Work, T. S.: The functional characterization of ribosomes from rat liver mitochondria. Biochem. biophys. Res. Commun. 39, 204–211 (1970).

    Google Scholar 

  • Bray, G. A.: A simple efficient liguid seintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem. 1, 279–285 (1960).

    Google Scholar 

  • Damsky, C. H., Nelson, W. M., Claude, A.: Mitochondria in anaerobically-grown, lipidlimited brewer's yeast. J. Cell Biol. 43, 174–179 (1969).

    Google Scholar 

  • Duell, E. A., Inoue, S., Utter, M. F.: Isolation and properties of intact mitochondria from spheroplasts of yeast. J. Bact. 88, 1762–1773 (1964).

    Google Scholar 

  • Edelman, M., Verma, J. M., Littauer, V. Z.: Mitochondrial rRNA from Aspergillus nidulans: characterization of a novel molecular species. J. molec. Biol. 49, 67–83 (1970).

    Google Scholar 

  • Fauman, M., Rabinowitz, M., Getz, G. S.: Base composition and sedimentation properties of mitochondrial RNA of Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 182, 355–360 (1969).

    Google Scholar 

  • Forrester, I. T., Nagley, P., Linnane, A. W.: Yeast mitochondrial ribosomal RNA: a new extraction procedure and unusual physical properties. FEBS. Lett. 11, 59–61 (1970).

    Google Scholar 

  • Girard, M., Latham, H., Penman, S., Darnell, J. E.: Entrance of newly formed messenger RNA and ribosomes into HeLa cell cytoplasm. J. molec. Biol. 11, 187–201 (1965).

    Google Scholar 

  • Grivell, L. A., Reijnders, L., Borst, P.: The effect of temperature and ionic strength on the electrophoretic mobility of yeast mitochondrial RNA. Europ. J. Biochem. 19, 64–72 (1971).

    Google Scholar 

  • Ito, K., Iwabuchi, M.: Conformational changes of ribosomal subunits of Dictyostelium discoideum by salts. J. Biochem. (Tokyo) 69, 1135–1138 (1971).

    Google Scholar 

  • Loening, U. E.: The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 113, 131–138 (1969).

    Google Scholar 

  • Morimoto, H., Halvorson, H.: Characterization of mitochondrial ribosomes from yeast. Proc. nat. Acad. Sci. (Wash.) 68, 324–338 (1971).

    Google Scholar 

  • Perlman, S., Penman, S.: Protein-synthesizing structures associated with mitochondria. Nature (Lond.) 227, 133–137 (1970).

    Google Scholar 

  • Schmitt, H.: Characterization of mitochondrial ribosomes from Saccharomyces cerevisiae. FEBS Lett. 4, 234–238 (1969).

    Google Scholar 

  • Schmitt, H.: Characterization of a 72S mitochondrial ribosome from Saccharomyces cerevisiae. Europ. J. Biochem. 17, 278–283 (1970).

    Google Scholar 

  • Scragg, A. H., Morimoto, H., Villa, V., Nekhorocheff, J., Halvorson, H.: Cell-free protein synthesizing system from yeast mitochondria. Science (N.Y.) 171, 908–910 (1971).

    Google Scholar 

  • Stegeman, W. J., Cooper, C. S., Avers, C. J.: Physical characterization of ribosomes from purified mitochondria of yeast. Biochem. biophys. Res. Commun. 39, 69–76 (1970).

    Google Scholar 

  • Yu, R. S., Poulson, R., Stewart, P. R.: Comparative studies on mitochondrial development in yeasts. I. Mitochondrial ribosomes from Candida parapsilosis. Molec. Gen. Genetics. 114, 325–338 (1972).

    Google Scholar 

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Author notes

  1. Rozanne Poulson

    Present address: Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver 8, B.C., Canada

Authors and Affiliations

  1. Department of Developmental Biology, Research School of Biological Sciences, Australian National University, Canberra, Australia

    R. Yu, Rozanne Poulson & P. R. Stewart

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  1. R. Yu
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  2. Rozanne Poulson
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  3. P. R. Stewart
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Communicated by H. G. Wittmann

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Yu, R., Poulson, R. & Stewart, P.R. Comparative studies on mitochondrial development in yeasts. Molec. Gen. Genet. 114, 339–349 (1972). https://doi.org/10.1007/BF00267502

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  • DOI: https://doi.org/10.1007/BF00267502

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