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Tn951: A new transposon carrying a lactose operon

Summary

A new transposon, Tn951, is described, which derives from plasmid pGC1, originally isolated from Yersinia enterocolitica. Tn951 is 16.6 kb long and presumably flanked by small inverted repeats. It carries the lac genes i, z and y. This lac system is homologous to the E. coli lac operon. However, homology is restricted to 5.6 kb. The DNA sequences surrounding the lac operons on Tn951 and E. coli are nonhomologous. This leads to speculations about the origin of the E. coli lac operon itself.

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References

  • Achtman, M.A., Willetts, N.S., Clark, A.J.: Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer deficient mutants. J. Bact. 106, 529 (1971)

    Google Scholar 

  • Bachman, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116 (1976)

    Google Scholar 

  • Barth, P.T., Datta, N., Hedges, R.W., Grinter, N.J.: Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R 483 to other replicons. J. Bact. 125, 800 (1976)

    Google Scholar 

  • Beckwith, J.R.: Lac: The genetic system. In: The lactose operon, p. 27. New York: Cold Spring Harbor Laboratory 1970

    Google Scholar 

  • Berg, D.E., Davies, J., Allet, B., Rochaix, J.D.: Transposition of R factor genes to bacteriophage lambda. Proc. nat. Acad. Sci. (Wash.) 72, 3628 (1975).

    Google Scholar 

  • Clowes, R.C., Hayes, W.: In: Experiments in microbial genetics, p. 225. Oxford: Blackwell 1968

    Google Scholar 

  • Cohen, S.N., Kopecko, D.J.: Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertion. Fed. Proc. 35, 2031 (1976)

    Google Scholar 

  • Cornelis, G., Bennett, P.M., Grinsted, J.: Properties of pGC1, a lac plasmid originating in Yersinia enterocolitica 842. J. Bact. 127, 1058 (1976)

    Google Scholar 

  • Cornelis, G., Colson, C.: Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli. J. gen. Microbiol 87, 285 (1975)

    Google Scholar 

  • Cornelis, G., Ghosal, D., Saedler, H.: Translocation and analysis of a plasmid DNA sequence encoding lactose fermentation. Proc. Soc. gen. Microbiol. 4, 151 (1977)

    Google Scholar 

  • Grinsted, J., Saunders, J., Ingram, L.C., Sykes, R.B., Richmond, M.H.: Properties of an R factor which originated in Pseudomonas aeruginosa 1822. J. Bact. 110, 529 (1972)

    Google Scholar 

  • Guiso, N., Ulmann, A.: Expression and regulation of lactose genes carried by plasmids. J. Bact. 127, 691 (1976)

    Google Scholar 

  • Hedges, R.W., Jacob, A.E.: Transposition of ampicillin resistance from RP4 to other replicons. Molec. gen. Genet. 132, 31 (1974)

    Google Scholar 

  • Hu, S., Ohtsubo, E., Davidson, N., Saedler, H.: Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequence IS1 and IS2 in F and R plasmids. J. Bact. 122, 764 (1975)

    Google Scholar 

  • Kondo, E., Mitsuhashi, S.: Drug resistance of enteric bacteria. IV. Active transducing bacteriophage P1CM produced by the recombination of R factor with bacteriophage P1. J. Bact. 88, 1266 (1964)

    Google Scholar 

  • Kopecko, D.J., Cohen, S.N.: Site-specific Rec A-independant recombination between bacterial plasmids: involvment of palindromes at the recombinational loci. Proc. nat. Acad. Sci. (Wash.) 72, 1373 (1975)

    Google Scholar 

  • Le Minor, L., Coynault, C., Pessoa, G.: Determinisme plasmidique du caractere atypique “lactose positif” de souches de Salmonella typhimurium et de Salmonella oranienbourg isolees au Bresil lors d'epidemies de 1971 a 1973. Ann. Microbiol. (Inst. Pasteur) 125A, 261 (1964)

    Google Scholar 

  • MacHattie, L., Jackowski: In: DNA insertion elements, plasmids and episomes (Bukhari, A.J., Shapiro, J., Adhia, S., ed.). New York: Cold Spring Harbor Laboratory p. 219 (1977)

    Google Scholar 

  • Michaelis, G., Saedler, H., Venkov, P., Starlinger, P.: Two insertions in the galactose operon having different sizes but homologous DNA sequences. Molec. gen. Genet. 104, 371 (1969)

    Google Scholar 

  • Miller, J.H.: In: Experiments in molecular genetics, pp. 352 and 319. New York: Cold Spring Harbour Laboratory 1972

    Google Scholar 

  • Palchaudhuri, S., Maas, W.K.: Physical mapping of a DNA sequence common to plasmids of incompatibility group F 1. Proc. nat. Acad. Sci. (Wash.) 74, 1190 (1977)

    Google Scholar 

  • Ptashne, K., Cohen, S.N.: Occurence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J. Bact. 122, 776 (1975)

    Google Scholar 

  • Richmond, M.H., Sykes, R.B.: The chromosomal integration of a β-lactamase gene drived from the P type R factor RPI in Escherichia coli. Genet. Res. 20, 231 (1972)

    Google Scholar 

  • Saedler, H., Heiss, B.: Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K12. Molec. gen. Genet. 122, 267–277 (1973)

    Google Scholar 

  • Sharp, P.A., Cohen, S.N., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of E. coli. II. Structure of drug resistance (R) factors and F factors. J. molec. Biol. 75, 235–255 (1973)

    Google Scholar 

  • Sharp, P.A., Hsu, M.T., Ohtsubo, E., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J. molec. Biol. 71, 471–497 (1972)

    Google Scholar 

  • Stanisich, V.A., Bennett, P.M., Ortiz, J.M.: A molecular analysis of transductional marker rescue involving P group plasmids in Pseudomonas aeruginosa. Molec. gen. Genet. 143, 335–337 (1976)

    Google Scholar 

  • Stanisich, V.A., Bennett, P.M., Richmond, M.H.: Characterization of a translocation unit encoding resistence to mercuric ions that occurs on a non-conjugative plasmid in Pseudomonas aeruginosa. J. Bact. 129, 1227–1233 (1977)

    Google Scholar 

  • Starlinger, P., Saedler, H.: IS-elements in microorganisms. Curr. Top. Microbiol. Immunol. 75, 111–152 (1976)

    Google Scholar 

  • Synenki, R.M., Wholieter, J.A., Johnson, E.M., Lazere, J.R., Baron, L.S.: Isolation and characterization of circular deoxyribonicleic acid obtained form lactose-fermenting Salmonella strains. J. Bact. 116, 1185–1190 (1973)

    Google Scholar 

  • Watanabe, T., Ocata, Y., Chan, R.K., Botstein, D.: Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage 22. Virol. 50, 874–882 (1972)

    Google Scholar 

  • Zabin, I., Fowler, A.W.: β-galactosidase and thiogalactosidase transacetylase. In: The lactose operon Beckwith and Lipser, p. 27. New York: Cold Spring Harbour Laboratory 1970

    Google Scholar 

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Communicated by E. Bautz

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Cornelis, G., Ghosal, D. & Saedler, H. Tn951: A new transposon carrying a lactose operon. Molec. Gen. Genet. 160, 215–224 (1978). https://doi.org/10.1007/BF00267484

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  • DOI: https://doi.org/10.1007/BF00267484

Keywords

  • Lactose
  • Inverted Repeat
  • Yersinia Enterocolitica
  • Lactose Operon
  • Plasmid pGC1