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A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA)

Molecular and General Genetics MGG volume 229, pages 341–352 (1991)Cite this article

Summary

The Escherichia coli acid phosphatase gene appA is expressed in response to oxygen deprivation and is positively controlled by the product of appR (katF) which encodes a putative new σ transcription-initiation factor. However, transcription of appA from its nearest promoter (P1) did not account for total pH 2.5 acid phosphatase expression and was not subject to regulation. The cloned region upstream of appA was extended and analyzed by insertions of transposon TnphoA and by fusions with lacZ. It contains two new genes, appC and appB, which both encode extracytoplasmic proteins. appC and appB are expressed from a promoter (P2) lying just upstream of appC. Both genes are regulated by oxygen, as is appA, and by appR gene product exactly as previously shown for appA. Analysis of the nucleotide sequence and of the origins of transcription have confirmed that the P2-appC-appB- (ORFX)-P1-appA region is organized on the chromosome as an operon transcribed clockwise from P2 and that P1 is a minor promoter for appA alone. Genes appC and appB encode proteins of Mr 58133 and 42377, respectively, which have the characteristics of integral membrane proteins. The deduced amino acid sequences of appC and appB show 60% and 57% homology, respectively, with subunits I and II of the E. coli cytochrome d oxidase (encoded by genes cydA and cydB). The notion that the AppC and AppB proteins constitute a new cytochrome oxidase or a new oxygen-detoxifying system is supported by the observation of enhanced sensitivity to oxygen of mutants lacking all three genes, cyo (cytochrome o oxidase), cyd (cytochrome d oxidase) and appB, compared to that of cyo cyd double mutants.

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Affiliations

  1. Service de Biochimie des Proteines, Laboratoire d'Ingénierie des Proteines, France

    Janie Dassa, Hafida Fsihi, Michel Dion, Muriel Kieffer-Bontemps & Paul L. Boquet

  2. Service de Biochimie et Génétique Moléculaire, C.E. Saclay, F-91191, Gif-sur-Yvette Cedex, France

    Christian Marck

Authors
  1. Janie Dassa
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  2. Hafida Fsihi
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  3. Christian Marck
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  4. Michel Dion
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  5. Muriel Kieffer-Bontemps
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  6. Paul L. Boquet
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Communicated by R. Devoret

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Dassa, J., Fsihi, H., Marck, C. et al. A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA). Molec. Gen. Genet. 229, 341–352 (1991). https://doi.org/10.1007/BF00267454

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  • DOI: https://doi.org/10.1007/BF00267454

Key words

  • Protein fusions
  • Promoters
  • Cytochrome oxidases
  • Oxygen toxicity