Summary
The exchange of ribosomal proteins among ribosomes of E. coli has been measured, using a density label technique. As expected most of the proteins do not exhange appreciably. However a substantial fraction of each of proteins S1, S2, S21, L7/L12, L9, L10, L11, L26 and L33 is found to exchange, but exchange of S1, S2, L7/L12, L10, L11 and L26 is found to occur in vitro after lysis of the cells, and therefore it is not possible to say whether or not these proteins also exchange in vivo. In contrast S21, L9 and L33 do not exchange after lysis of the cells and we therefore conclude that these proteins exchange in vivo. The maximum level of exchange of S21, L9 and L33 is attained so rapidly that we were unable to show whether or not it was dependent on protein synthesis.
Similar content being viewed by others
References
Cochran, W.G.: Approximate significance levels of the Behrens-Fisher test. Biometrics 20, 191–195 (1964)
Davern, C.I., Meselson, M.: The molecular conservation of RNA during bacterial growth. J. molec. Biol. 2, 153–160 (1960)
Dennis, P.P.: In vivo stability, maturation and relative differential synthesis rates of individual ribosomal proteins in E. coli B/r. J. molec. Biol. 88, 25–41 (1974)
Dohme, F., Nierhaus, K.H.: Total reconstitution and assembly of 50S subunits from E. coli ribosomes in vitro. J. molec. Biol. 107, 585–599 (1976)
Duin, J. van, Knippenberg, P.H. van, Dieben, M., Kurland, C.G.: Functional heterogeneity of the 30S ribosomal subunit of E. coli. Molec. gen. Genet. 116, 181–191 (1972)
Garrett, R.A., Müller, S., Spierer, P., Zimmermann, R.A.: Binding of 50S ribosomal subunit proteins to 23S RNA of E. coli. J. molec. Biol. 88, 553–557 (1974)
Garrett, R.A., Rak, K.H., Daya, L., Stöffler, G.: Ribosomal proteins. XXIX. Specific protein binding sites on 16S rRNA of E. coli. Molec. gen. Genet. 114, 112–124 (1971)
Hardy, S.J.S.: The stoichiometry of the ribosomal proteins of E. coli. Molec. gen. Genet. 140, 253–274 (1975)
Held, W.A., Ballou, B., Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal proteins from E. coli. J. biol. Chem. 249, 3103–3111 (1974a)
Held, W.A., Nomura, M., Hershey, J.W.B.: Ribosomal protein S21 is required for full activity in the initiation of protein synthesis. Molec. gen. Genet. 128, 11–22 (1974b)
Homann, H.E., Nierhaus, K.H.: Protein compositions of biosynthetic precursors and artificial subparticles from ribosomal subunits in E. coli K12. Europ. J. Biochem. 20, 249–257 (1971)
Kaempfer, R.O.R., Meselson, M., Raskas, H.J.: Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. J. molec. Biol. 31, 277–289 (1968)
Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal proteins from E. coli. Nature (Lond.) 226, 1214–1218 (1970)
Pichon, J., Coeroli, C., Marchis-Mouren, G.: Studies on ribosomal protein biosynthesis in an RNA polymerase temperature sensitive E. coli mutant. Molec. gen. Genet. 150, 257–264 (1977)
Pichon, J., Marvaldi, J., Marchis-Mouren, G.: The in vivo order of protein addition in the course of E. coli 30S and 50S subunit biogenesis. J. molec. Biol. 96, 125–137 (1975)
Schaup, H.W., Green, M., Kurland, C.G.: Molecular interactions of ribosomal components. I. Identification of RNA binding sites for individual 30S ribosomal proteins. Molec. gen. Genet. 109, 193–205 (1970)
Stöffler, G., Daya, L., Rak, K.H., Garrett, R.A.: Ribosomal proteins. XXVI. The number of specic protein binding sites on 16S and 23S RNA of E. coli. J. molec. Biol. 62, 411–414 (1971)
Subramanian, A.R.: Copies of proteins L7 and L12 and heterogeneity of the large subunit of E. coli ribosome. J. molec. Biol. 95, 1–8 (1975)
Weber, H.J.: Stoichiometric measurements of 30S and 50S ribosomal proteins from E. coli. Molec. gen. Genet. 119, 233–248 (1972)
Wittmann, H.G.: Purification and identification of E. coli ribosomal proteins. In: Ribosomes (Nomura, Tissières, Lengyel, eds.), pp. 93–114. Cold Spring Harbor Monograph Series: New York: Cold Spring Harbor Laboratory 1974
Zimmermann, R.A.: RNA-protein interactions in the ribosome. In: Ribosomes (Nomura, Tissières, Lengyel, eds.), pp. 225–269. Cold Spring Harbor Monograph Series: New York: Cold Spring Harbor Laboratory 1974
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Robertson, W.R., Dowsett, S.J. & Hardy, S.J.S. Exchange of ribosomal proteins among the ribosomes of Escherichia coli . Molec. Gen. Genet. 157, 205–214 (1977). https://doi.org/10.1007/BF00267399
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267399