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The araC regulatory gene mRNA contains a leader sequence

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Summary

An estimation of the size of the araC gene in Escherichia coli B/r was made by sub-cloning restriction fragments of the araC-containing hybrid plasmid pTB1 into the plasmid pBR322. Plasmids which contained a functional araC gene were identified by genetic complementation tests. DNA sequence analysis of the promoter-proximal region of the araC gene revealed that araC mRNA contains a 150 nucleotide leader.

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References

  • Casadaban M (1976) Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol 104:557

    Google Scholar 

  • Cohen SN, Chang ACY, Heu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2119

    Google Scholar 

  • Englesberg E (1971) Regulation of the L-arabinose system. In: Vogel H (ed) Metabolic pathways, vol 5. Academic Press, Inc., New York, p 257

    Google Scholar 

  • Englesberg E, Irr J, Power J, Lee N (1965) Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol 90:946–957

    Google Scholar 

  • Englesberg E, Wilcox G (1974) Regulation: Positive control. Annu Rev Genet 8:219–242

    Google Scholar 

  • Greenfield L, Boone T, Wilcox G (1978) DNA sequence of the araBAD promoter in Escherichia coli B/r. Proc Natl Acad Sci USA 75:4724–4728

    Google Scholar 

  • Horwitz AH, Morandi C, Wilcox G (1980) Deoxyribonucleic acid sequence of araBAD promoter mutants of Escherichia coli. J Bacteriol 142:659–667

    Google Scholar 

  • Iserentant D, Fiers W (1980) Secondary structure of mRNA and efficiency of translation initiation. Gene 9:1–12

    Google Scholar 

  • Johnson RA, Walseth TF (1979) The enzymatic preperation of [α-32P]ATP, [α-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases. In: Brooker G, Greengard P, Robison GA (eds) Advances in Cyclic Nucleotide Research, vol 10. Raven Press, New York, p 135–167

    Google Scholar 

  • Johnston HM, Barnes WM, Chumley FG, Bossi L, Roth J (1980) Model for regulation of the histidine operon of Salmonella. Proc Natl Acad Sci USA 77:508–512

    Google Scholar 

  • Kaplan D, Greenfield L, Boone T, Wilcox G (1978) Hybrid plasmids containing the araCBAD genes of Escherichia coli. Gene 3:177–189

    Google Scholar 

  • Keller EB, Calvo JM (1979) Alternative secondary structures of leader RNAs and the regulation of the trp, phe, his, thr, and leu operons. Proc Natl Acad Sci USA 76:6186–6190

    Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end labeled DNA with base-specific chemical clevages. In: Grossman L, Moldave K (eds). Methods in enzymology, vol 65:Academic Press, Inc., New York, p 499

    Google Scholar 

  • Murray K, Hughes SG, Brown JS, Bruce S (1976) Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis. Biochem J 159:317–322

    Google Scholar 

  • Norgard M, Emigholz K, Monahan J (1979) Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and X1776 grown in the presence of high concentrations of nucleoside. J Bacteriol 138:270–272

    Google Scholar 

  • Oxender DL, Zurawski G, Yanofsky C (1979) Attenuation in the Escherichia coli tryptophan operon: Role of RNA secondary structure involving the tryptophan codon region. Proc Natl Acad Sci USA 76:5524–5529

    Google Scholar 

  • Rodriguez RL, West RW, Heyneker HL, Bolivar F, Boyer HW (1979) Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. Nucleic Acids Res 6:3267–3287

    Google Scholar 

  • Sato S, Hutchison III CA, Harris II (1977) A thermostable sequence-specific endonuclease from Thermus aquaticus. Proc Natl Acad Sci USA 74:542–546

    Google Scholar 

  • Smith B, Schleif R (1978) Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12. J Biol Chem 253:6931–6933

    Google Scholar 

  • Steitz JA (1979) Genetic signals and nucleotide sequences in messenger RNA. In: Goldberger R (ed) Biological regulation and development, vol 1, Gene expression. Plenum Press, New York, p 349

    Google Scholar 

  • Studnicka GM, Rahn GM, Cummings IW, Salser WA (1978) Computer method for predicting the secondary structure of single-stranded RNA. Nucleic Acids Res 5:3365–3386

    Google Scholar 

  • Suteliffe JG (1978) pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res 5:2721–2728

    Google Scholar 

  • Wilcox G, Boulter J, Lee N (1974) Direction of transcription of the regulatory gene araC in Escherichia coli B/r. Proc Natl Acad Sci USA 71:3635

    Google Scholar 

  • Wilcox G, Clementson KJ, Sante D, Englesberg E (1971) Purification of the araC protein. Proc Natl Acad Sci USA 68:2145 (1971)

    Google Scholar 

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Communicated by H.W. Boyer

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Cass, L.G., Horwitz, A.H., Miyada, C.G. et al. The araC regulatory gene mRNA contains a leader sequence. Molec. Gen. Genet. 180, 219–226 (1980). https://doi.org/10.1007/BF00267373

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  • DOI: https://doi.org/10.1007/BF00267373

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