Summary
A mutation in the cyR1 gene of the fungus Podospora anserina confers resistance to cycloheximide and leads to an alteration of the 60S ribosomal protein L21 (Bégueret et al. 1977). Nine revertants of this mutant were isolated and the properties of these strains were analyzed. It was found that one revertant strain contains a new mutant form of L21. It is proposed that the cyR1 gene is the structural gene for protein L21 and that the alteration of this protein is responsible for the resistance to cycloheximide in vivo.
Similar content being viewed by others
References
Bégueret J, Perrot M, Crouzet M (1977) Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant. Mol Gen Genet 156:141–144
Bernet J (1965) Mode d'action des gènes de barrage et relation entre l'incompatibilité cellulaire et l'incompatibilité sexuelle chez le Podospora anserina. Ann Sci Nat Bot 12:611–768
Crouzet M (1978) Thèse 3° cycle. Université de Bordeaux II
Crouzet M, Perrot M, Nogueira M, Bégueret J (1978) Genetic and biochemical analysis of cycloheximide resistance in the fungus Podospora anserina. Biochem. Genet 16:271–286
Crouzet M, Bégueret J (1978) Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina. Mol Gen Genet 165:283–288
Crouzet M, Bégueret J (1980) Altered ribosomal proteins in mutants of the fungus Podospora anserina. J Biol Chem 255:4996–4999
Coddington A, Fluri R (1977) Characterization of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis: demonstration that a cycloheximide resistant strain, cyhl, has an altered 60S ribosomal protein. Mol Gen Genet 158, 93–100
Esser K (1974) Podospora anserina. In: King RC (ed) Handbook of genetics, vol 1. Plenum, New York, p 531
Funatsu G, Wittmann HG (1972) Ribosomal proteins. XXXIII. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistan to streptomycin. J Mol Biol 68:547–550
Henderson LE, Oroszlan S, Konigsberg W (1979) A micromethod for complete removal of dodecyl sulfate from proteins by ionpair extraction. anal Biochem 93:153–157
Howard G, Traut R (1973) Separation and radio-autography of microgram quantities of ribosomal proteins by two dimensional polyacrylamide gel electrophoresis. FEBS Letters 29:177–180
Isono S, Isono K, Hirota Y (1978) Mutation affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli. Mol Gen Genet 165:15–20
Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting ribosomal proteins. Anal Biochem 36:401–412
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Marcu K, Dudock B (1974) Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucl Acids Res 1:1385–1397
Marcus A, Bewley JD, Weeks DP (1970) Aurintricarbolylic acid and initiation factors of wheat embryo. Science 167:1735–1736
Sherton CC, Wool IG (1974) The extraction of proteins from eukaryotic ribosomes and ribosomal subunits. Mol Gen Genet 135:97–112
Wittmann HG, Wittmann-Liebold B (1974) Chemical structure of bacterial ribosomal proteins. In: Nomura M, Tissieres A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, New York, p 115
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Crouzet, M., Begueret, J. A new mutant form of the ribosomal protein L21 in the fungus Podospora anserina: Identification of the structural gene for this protein. Molec. Gen. Genet. 180, 177–183 (1980). https://doi.org/10.1007/BF00267367
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267367