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Isolation of E. coli mutants containing multiple transpositions of IS sequences

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Summary

The characterization of three E. coli mutants that appeared to have unselected IS1 insertions on the chromosome are described. One had a single new IS1 sequence. The second had three new IS1 sequences. The third had two new IS1 sequences and one of the IS1 sequences in the parent was missing. These mutants were found in a collection of strains that contained IS insertions in the spc operon. The frequency of finding mutants with unselected IS1 transpositions was at least 100 times greater than expected. The results suggest several transposition events may frequently occur in the same cell.

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References

  • Ahmed A (1977) The gal3 mutation of E. coli. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 37

    Google Scholar 

  • Bennett PM, Grinsted J, Richmond MH (1977) Transposition of TnA does not generate deletions. Mol Gen Genet 154:205–211

    Google Scholar 

  • Blattner FR, Fiandt M, Hass KK, Twose PA, Szybalski W (1974) Delections and insertions in the immunity region of coliphage lambda: Revised measurement of the promoter-startpoint distance. Virology 62:458–471

    Google Scholar 

  • Brachet P, Eisen H, Rambach A (1970) Mutations of coliphage λ affecting the expression of replicative functions O and P. Mol Gen Genet 108:266–276

    Google Scholar 

  • Chadwell H, Fritz H-J, Habermann P, Klaer R, Kühn S, Starlinger P (1979) Studies with IS1 and IS4. Cold Spring Harbor Symp Quant Biol 43:1187–1192

    Google Scholar 

  • Cornelis G, Saedler H (1980) Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951. Mol Gen Genet (in press)

  • Daniels DL, DeWet SR, Blattner FR (1980) New map of bacteriophage lambda DNA. J Virol 33:390–400

    Google Scholar 

  • Faelen M, Toussaint A, De Lafonteyne J (1975) Model for the enhancement of λ gal integration into partially induced Mu-1 lysogens. J Bacteriol 121:873–882

    Google Scholar 

  • Fiandt M, Szybalski W, Malamy MH (1972) Polar mutations in lac, gal and phage λ consist of a few IS-DNA sequences inserted with either orientation. Mol Gen Genet 119:223–231

    Google Scholar 

  • Fiandt M, Szybalski W, Blattner FR, Jaskunas SR, Lindahl L, Nomura M (1976) Organization of ribosomal protein genes in E. coli 1. Physical structure of DNA from transducing λ phages carrying genes from the aroE-str region. J Mol Biol 106:817–835

    Google Scholar 

  • Foster TJ (1976) R factor-mediated tetracycline resistance in Escherichia coli K12. Dominance of some tetracycline sensitive mutants and relief of dominance by selection. Mol Gen Genet 143:339–344

    Google Scholar 

  • Ghosal D, Sommer H, Saedler H (1979) Nucleotide sequence of the transposable DNA-element IS2. Nucleic Acids Res 6:1111–1122

    Google Scholar 

  • Gill R, Heffron F, Dougan G, Falkow S (1978) Analysis of sequences transposed by complementation of two classes of transposition deficient mutants of Tn3. J Bacteriol 136:742–756

    Google Scholar 

  • Gill RE, Heffron F, Falkow S (1979) Identification of the protein encoded by the transposable element Tn3 which is required for its transposition. Nature (London) New Biol 282:797–801

    Google Scholar 

  • Gottesman MM, Rosner JL (1975) Acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc Natl Acad Sci USA 72:5041–5045

    Google Scholar 

  • Grindley NDF, Sherratt D (1978) Sequence analysis at IS1 insertion sites: Model for transposition. Cold Spring Harbor Symp Quant Biol 43:1257–1261

    Google Scholar 

  • Hu S, Ohtsubo E, Davidson N, Saedler H (1975) Electron microscope heteroduplex studies of sequence relations among plasmids: Identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J Bacteriol 122:764–775

    Google Scholar 

  • Jaskunas SR, Lindahl L, Nomura M (1975) Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli. Nature (London) New Biol 256:183–187

    Google Scholar 

  • Jaskunas SR, Nomura M (1977a) Mapping ribosome protein genes in E. coli by means of insertion mutations. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory: Cold Spring Harbor, New York, p 487

    Google Scholar 

  • Jaskunas SR, Nomura M (1977b) Organization of ribosomal protein genes of E. coli as analyzed by polar insertion mutations. J Biol Chem 252:7337–7343

    Google Scholar 

  • Johnsrud L (1979) DNA sequence of the transposable element IS1. Mol Gen Genet 169:213–218

    Google Scholar 

  • Jordan E, Saedler H, Starlinger P (1968) 00-and strong-polar mutations in the gal operon are insertions. Mol Gen Genet 102:353–363

    Google Scholar 

  • Kleckner N (1977) Translocatable elements in procaryotes. Cell 11:11–23

    Google Scholar 

  • Kretschmer PJ, Cohen SN (1979) Effect of temperature on translocation frequency of the Tn3 element. J Bacteriol 139:515–519

    Google Scholar 

  • Ljungquist E, Bukhari AI (1977) State of prophage Mu DNA upon induction. Proc Natl Acad Sci USA 74:3143–3147

    Google Scholar 

  • MacHattie L, Jackowski JB (1977) Physical structure and deletion effects of the chloramphenicol resistance element Tn9 in phage lambda. In: Bukhari AI, Shapiro JA, Adhya SL (eds) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 219

    Google Scholar 

  • Malamy MH (1970) Some properties of insertion mutations in the lac operon. In: Beckwith JR, Zipser D (eds) The lactose operon. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 359

    Google Scholar 

  • Mickel S, Ohtsubo E, Bauer W (1977) Heteroduplex mapping of small plasmids derived from R-factor R-12: In vivo recombination occurs at IS1. Gene 2:193–219

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Nyman K, Nakamura K, Ohtsubo H, Ohtsubo E (1980) Distribution of the insertion sequence IS1 in gram-negative bacteria: Origin of IS1 and evolution of resistance and enterotoxin plasmids. Nature (London), New Biol (in press)

  • Ohtsubo H, Ohtsubo E (1978) Nucleotide sequences of an insertion elements. IS1. Proc Natl Acad Sci USA 75:615–619

    Google Scholar 

  • Ptashne K, Cohen SN (1975) Occurrence of insertion sequences (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol 122:776–781

    Google Scholar 

  • Read HA, DasSarma S, Jaskunas SR (1980) Fate of donor insertion sequence IS1 during transposition. Proc Natl Acad Sci USA 77:2514–2518

    Google Scholar 

  • Reif HJ, Saedler H (1975) IS1 is involved in deletion formation in the gal region of E. coli K-12. Mol Gen Genet 137:17–28

    Google Scholar 

  • Ross DG, Swan J, Kleckner N (1979) Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitions. Cell 16:721–731

    Google Scholar 

  • Rownd RH, Perlman D, Goto N, Appelbaum ER (1975) Control of RTF and r-determinant replication in composite R plasmids. In: Goulian M, Hanawalt P, Fox CF (eds) DNA synthesis and its regulation. WA Benjamin Inc, Menlo Park, California, p 537

    Google Scholar 

  • Saedler H, Heiss B (1973) Multiple copies of the insertion DNA sequences IS1 and IS2 in the chromosome of E. coli K-12. Mol Gen Genet 122:267–277

    Google Scholar 

  • Saedler H, Kubai DF, Nomura M, Jaskunas SR (1975) IS1 and IS2 mutations in the ribosomal protein genes of E. coli K12. Mol Gen Genet 141:85–89

    Google Scholar 

  • Shapiro J (1969) Mutations caused by the insertion of genetic material into the galactase operon of Escherichia coli. J Mol Biol 40:93–105

    Google Scholar 

  • Shapiro JA (1979) Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci USA 76:1933–1937

    Google Scholar 

  • Shapiro JA, MacHattie LA (1979) Integration and excision of prophage λ mediated by the IS1 element. Cold Spring Harbor Symp Quant Biol 43:1135–1142

    Google Scholar 

  • Shinnick TM, Lund E, Smithies P, Blattner FR (1975) Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res 2:1911–1929

    Google Scholar 

  • So M, Heffron F, McCarthy BJ (1979) The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature (London) New Biol 277:453–456

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Starlinger P, Saedler H (1976) IS-elements in microorganisms. Curr Top Microbiol Immunol 75:111–152

    Google Scholar 

  • Tu C-PD Cohen SN (1980) Translocation specificity of the Tn3 element: Characterization of sites of multiple insertions. Cell 19:151–160

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Indiana University, 47401, Bloomington, Indiana, USA

    Holly A. Read & S. Richard Jaskunas

  2. the Program in Molecular, Cellular and Developmental Biology, Indiana University, 47401, Bloomington, Indiana, USA

    Holly A. Read & S. Richard Jaskunas

Authors
  1. Holly A. Read
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  2. S. Richard Jaskunas
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Communicated by H. Saedler

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Read, H.A., Jaskunas, S.R. Isolation of E. coli mutants containing multiple transpositions of IS sequences. Molec. Gen. Genet. 180, 157–164 (1980). https://doi.org/10.1007/BF00267365

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  • DOI: https://doi.org/10.1007/BF00267365

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