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Rheumatology International

, Volume 7, Issue 1, pp 35–41 | Cite as

Diverse morphological responses of normal human synovial fibroblasts to mononuclear leukocyte products: relationship to prostaglandin production and plasminogen activator activities, and comparison with the effects of purified interleukin 1

  • B. J. Clarris
  • T. Leizer
  • J. R. E. Fraser
  • J. A. Hamilton
Originals

Summary

Supernatant media from cultured human mononuclear blood leukocytes (MCCM) induced morphological changes in normal human synovial fibroblasts in culture, including the formation of cells with a dendritic or stellate morphology and, less frequently, cells with a striking fenestrated appearance. These changes were fully reversed within 1 h of removing the MCCM. They were inhibited by indomethacin, the glucocorticoids hydrocortisone, prednisolone and dexamethasone, and by colcemid, but not by actinomycin D and only weakly by cycloheximide. The morphological responses to MCCM could be reproduced by MCCM fractions containing interleukin 1-like activity and by purified forms of human interleukin 1 (IL-1), including monocyte-derived IL-1β and recombinant IL-1α. These responses were also inhibited by indomethacin, indicating a link with prostanoid production. However, the morphological responses were not related to the stimulation of plasminogen activator activity due to MCCM, MCCM fractions, or IL-1.

Key words

Synovial fibroblasts Morphology Prostanoids Mononuclear cells Interleukin 1 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • B. J. Clarris
    • 1
  • T. Leizer
    • 1
  • J. R. E. Fraser
    • 1
  • J. A. Hamilton
    • 1
  1. 1.Department of Medicine, Royal Melbourne HospitalUniversity of MelbourneParkvilleAustralia

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