Summary
A mutant has been isolated which carries a nuclear mutation capable of suppressing certain aspects of the phenotype imposed by a specific mitochondrial mutation. The mitochondrial mutation [tso-r] confers cold sensitivity to growth on nonfermentable substrates and resistance to oligomycin. When both the mitochondrial and nuclear mutations are present in the same cell the cell is phenotypically cold resistant but retains a high level of oligomycin resistance. The extent of cold sensitivity suppression is dependent upon other unspecified nuclear genes. The molecular basis for the suppression may involve interactions between cytoplasmic and mitochondrial products necessary for the assembly of mitochondrial ATPase.
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Casey, J. W., Hsu, H. J., Getz, G. S., Rabinowitz, M., Fukuhara, H.: Transfer RNA genes in mitochondrial DNA of grande (wild-type) yeast. J. molec. Biol. 88, 735–749 (1974)
Colson, A. M., Goffeau, A., Briquet, M., Weigal, P., Mattoon, J. R.: Nucleo-cytoplasmic interaction between oligomycin-resistant mutations in Saccharomyces cerevisiae. Molec. gen. Genet. 135, 309–326 (1974)
Linnane, A. W., Kellerman, G. M., Lukins, H. B.: In: Techniques of Biochemical and Biophysical Morphology (Glick, D., Rosenbaum, R., eds.), vol. 2, p. 1–98. New York: John Wiley & Sons Inc. 1975
Ono, B., Fink, G., Schatz, G.: Mitochondrial assembly in respiratory deficient mutants of Saccharomyces cerevisiae. IV. Effects of nuclear amber suppressor on the accumulation of a mitochondrially made subunit of cytochrome c oxidase. J. biol. Chem. 250, 775–782 (1975)
Trembath, M. K., Monk, B. C., Kellerman, G. M., Linnane, A. W.: Biogenesis of Mitochondria 36. The genetic and biochemical analysis of a mitochondrially determined cold sensitive oligomycin resistant mutant of Saccharomyces cerevisiae with affected mitochondrial ATPase assembly. Molec. gen. Genet, in press (1975)
Tzagoloff, A., Meagher, P.: Assembly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J. biol. Chem. 247, 594–503 (1972)
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Communicated by W. Gajewski
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Trembath, M.K., Monk, B.C., Kellerman, G.M. et al. Biogenesis of mitochondria 40. Molec. Gen. Genet. 140, 333–337 (1975). https://doi.org/10.1007/BF00267323
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DOI: https://doi.org/10.1007/BF00267323