Summary
The irradiation with visible light (Li) of temperate Serratiaphage that is maximally sensitized with either proflavine (PF) or methylene blue (MB) induces—apart from lethal lesions—mutations which are phenotypically expressed as clear (c) or lightly turbid (l) plaques. The mutagenicity of the MB+Li and PF+Li treatment differs in several respects: (i) Up to an inactivation of 6 to 8 lethal hits MB+Li is a much more potent mutagen than PF+Li. (ii) at low levels of survival the dose curve of mutation frequency with MB+Li reaches a peak and then decreases drastically while the mutation frequency after PF+Li continues to increase in proportion to lethal hits induced. (iii) Mapping of 100 MB+Li and 77 PF+Li induced c or l mutants indicates significant difference in the electiveness of the four genomic regions of c or l mutants.
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Communicated by F. Kaudewitz
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Brendel, M. Different photodynamic action of proflavine and methylene blue on bacteriophage. Molec. Gen. Genet. 120, 171–180 (1973). https://doi.org/10.1007/BF00267245
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DOI: https://doi.org/10.1007/BF00267245