Summary
Phage mutants were isolated with amber mutations in genes necessary for establishment of lysogeny. These mutants form turbid plaques on su + strain 527R1 and clear plaques of different types on LT2. According to complementation tests, fourteen mutants fall in the c 2 gene, four in the c 3 gene but no amber mutants were found belonging to the c 1 gene. Pulse labelling experiments to follow DNA synthesis after phage infection were done with the mutants classified by complementation tests. Furthermore the labelling experiments demonstrated that the nonleaky c 3 amber mutants displayed the same DNA synthesis pattern as c 1 missense mutants. Since these c 3 amber mutants complement missense c 1 mutants it is concluded that the c 3 and c 1 genes must act together for the first transient repression of DNA synthesis, i.e., seven minutes after infection. It is suggested that clear plaque forming c 1 amber mutants cannot be isolated because of polarity leading to defectivity of lysogenic as well as of lytic functions.
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Communicated by G. Bertani
The majority of the experiments presented are a part of the dissertation of H. D. Dopatka at the University of Göttingen.
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Dopatka, H.D., Prell, H.H. Amber mutants of Salmonella-phage P22 in genes engaged in the establishment of lysogeny. Molec. Gen. Genet. 120, 157–170 (1973). https://doi.org/10.1007/BF00267244
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DOI: https://doi.org/10.1007/BF00267244