Summary
When crossed to strain GR25 [rho+], petites lacking in mtDNA were neutral, but when crossed to a related [rho+] strain (GR25a) they were found to be suppressive (Table 1). Likewise, crosses of GR25 [rho+] and GR25a [rho+] to a common [rho+] parent were, respectively, neutral and suppressive (Table 1). The suppressive phenotype observed in these crosses was attributed to a factor in the [rho+] strain GR25a. Strain GR25a also differed from strain GR25 in having a decreased [rho+] stability (Table 2) and a decreased transmission of its cytoplasmically-inherited erythromycin-resistance marker to zygote progeny (Table 4). These three phenotypes of GR25a are, discussed in terms of a nuclear mutation in a gene responsible for the maintenance of the [rho+] state.
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Bech-Hansen, N.T., Rank, G.H. The bivious suppressiveness of cytoplasmic petites of S. cerevisiae lacking in mitochondrial DNA. Molec. Gen. Genet. 120, 115–124 (1973). https://doi.org/10.1007/BF00267239
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DOI: https://doi.org/10.1007/BF00267239