Summary
The length of the terminal redundant regions in T7 DNA has been determined by two methods. One involved the specific labeling and isolation of the redundant DNA fragment and determination of the molecular weight by polyacrylamide gel electrophoresis. A value of 150±10 nucleotide pairs was obtained. The other determination based on a correlation of the melting temperature of the redundant region to that of whole T7 DNA confirmed the result obtained by the first method.
References
Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in enzymologie XXIX, part E (L. Grossman and K. Moldave, eds.), pp. 363–418. New York-London: Academic Press 1974
Burd, J.F., Wartell, R.M., Dodgson, J.B., Wells, R.D.: Transmission of stability (telestability) in deoxyribonucleic acid. J. Biol. Chem. 250, 5109–5113 (1975)
Crothers, D.M., Kallenbach, N.R., Zimm, B.H.: The melting transition of low-molecular-weight DNA: theory and experiment. J. Mol. Biol. 11, 802–820 (1965)
Dreiseikelmann, B., Eichenlaub, R., Wackernagel, W.: The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and λ DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis. Biochim. Biophys. Acta 562, 418–428 (1979)
Dreiseikelmann, B., Wackernagel, W.: The terminal redundant regions of bacteriophage T7 DNA: their necessity for phage production studies by the infectivity of T7 DNA after modification by various exonucleases. Mol. Gen. Genet. 159, 321–328 (1978)
Ehrlich, S.D., Sagaramella, V., Lederberg, J.: Transfection of restrictionless Escherichia coli by bacteriophage T7 DNA: Effect of in vitro erosion of DNA by λ exonuclease. J. Mol. Biol. 105, 603–609 (1976)
Glynn, I.M., Chappel, J.B.: A simple method for the preparation of 32P-labeled adenosine triphosphate of high specific activity. Biochem. J. 90, 147–149 (1964)
Hausmann, R.: Bacteriophage T7 genetics. Curr. Top. Microbiol. Immunol. 75, 77–110 (1976)
Langman, L., Paetkau, V., Scraba, D., Miller, R.C., Jr., Roeder, G.S., Sadowski, P.D.: The structure and maturation of intermediates in bacteriophage T7 DNA replication. Can. J. Biochem. 56, 508–516 (1978)
Little, J.W., Lehman, I.R., Kaiser, A.D.: An exonuclease induced by bacteriophage λ, I. Preparation of the crystalline enzyme. J. Biol. Chem. 242, 672–678 (1967)
Ludwig, A.R., Summers, W.C.: A restriction fragment analysis of the T7 left early region. Virology 68, 360–373 (1975)
McDonell, S.W., Simon, M.N., Studier, F.W.: Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J. Mol. Biol. 110, 119–146 (1977)
Richardson, C.C.: Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected E. coli. Proc. Natl. Acad. Sci. U.S.A. 54, 158–165 (1965)
Richardson, C.C., Lehman, J.R., Kornberg, A.: A DNA phosphatase-exonuclease from E. coli. II. Characterization of the exonuclease activity. J. Biol. Chem. 239, 251–258 (1964)
Ritchie, D.A., Thomas, C.A., McHattie, L.A., Wensink, P.C.: Terminal repetition in non-permutated T3 and T7 bacteriophage DNA molecules. J. Mol. Biol. 23, 365–376 (1967)
Seroka, K., Wackernagel, W.: In vivo effects of recBC DNase, exonuclease I, and DNA polymerase of Escherichia coli on the infectivity of native and single-stranded DNA of bacteriophage T7. J. Virol. 21, 906–912 (1977)
Studier, F.W.: Bacteriophage T7. Science 176, 367–376 (1972)
Sussenbach, J.S., Monfoort, C.H., Schiphof, R., Stobberingh, E.E.: A restriction endonuclease from Staphylococcus aureus. Nucleic Acids Res. 3, 3193–3202 (1976)
Sutcliffe, J.G.: pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 5, 2721–2728 (1978)
Vogt, V.M.: Purification and further properties of single-strandspecific nuclease from Aspergillus oryzae. Eur. J. Biochem. 33, 192–200 (1973)
Watson, J.D.: Origin of concatemeric T7 DNA. Nature 239, 197–201 (1972)
Wilson, D.A., Thomas, C.A., Jr.: Palindromes in chromosomes. J. Mol. Biol. 84, 115–144 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by T. Yura
Rights and permissions
About this article
Cite this article
Dreiseikelmann, B., Steger, U. & Wackernagel, W. Length determination of the terminal redundant regions in the DNA of phage T7. Molec. Gen. Genet. 178, 237–240 (1980). https://doi.org/10.1007/BF00267236
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267236