Summary
P1 infected minicells synthesize approximately 50 phage-encoded polypeptides. Phage expression is temporally controlled, demonstrating phage polypeptides synthesized both early and late after infection. The P1 repressor, gpc1 1 (Mr=33,000), repressor bypass polypeptide, gprebA (Mr=27,500) and cistron 10 product, (gp10) (Mr=64,000), have been identified by infection of minicells with P1 amber mutants. The beta-lactamase gene product (gpbla) carried by the closely related phage P7 and the chloramphenicol acetyl-transferase gene product (gpcat) carried by P1 Cm (in Tn9) have been demonstrated. Infection of minicells by P1vir s or P1c4 mutants results in increased synthesis of gprebA and a second polypeptide designated gprebB (Mr=40,000). The P1vir11 mutation leads to increased synthesis of a small polypeptide (Mr=3,500) but does not affect the amount of gpc1 synthesized.
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Heilmann, H., Reeve, J.N. & Pühler, A. Identification of the repressor and repressor bypass (antirepressor) polypeptides of bacteriophage P1 synthesized in infected minicells. Molec. Gen. Genet. 178, 149–154 (1980). https://doi.org/10.1007/BF00267223
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DOI: https://doi.org/10.1007/BF00267223