Summary
A cleavage map of Tn5, a kanamycin (Km) transposon from plasmid JR67, was constructed from pMKI, a composite plasmid of ColE1 and Tn5, and compared to that of Tn903, a Km transposon from plasmid R6-5. The two transposons showed marked heterogeneity in both the structural gene for Km resistance and the inverted repeat regions as evidenced by their distinctly different restriction maps. This result suggests separate paths of evolution for the two Km transposons.
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Berg, D.E., Davies, J., Allet, B., Rochaix, J.D.: Transposition of R factor genes to bacteriophage λ. Proc. Natl. Acad. Sci. U.S.A. 72, 3628–3632 (1975)
Berg, D.E., Drummond, M.:Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12. J. Bacteriol. 136, 419–422 (1978)
Berg, D.E., Jorgensen, R., Davies, J.: Transposable kanamycinneomycin resistance derminants. In: Microbiology-1978 (D.Schlessinger, ed.) pp. 13–15. Wash. D.C.: Amer. Soc. Micro., 1978
Bolivar, F., Rodriguez, R.L., Greene, P.J. Betlach, M.C., Heyneker, H.L., Boyer, H.W.: Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 2, 95–113 (1977)
Chang, A.C.Y., Cohen, S.N.: Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141–1156 (1978)
Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature 263, 731–738 (1976)
Cohen, S.N., Chang, A.C.Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)
Dougan, G., Saul, M., Warren, G., Sherratt, D.: A functional map of plasmid ColE1. Mol. Gen. Genet. 158, 325–327 (1978)
Heffron, F., Bedinger, P., Champoux, J.J., Falkow, S.: Deletions affecting the transposition of an antibiotic resistance gene. Proc. Natl. Acad. Sci. U.S.A. 74, 702–706 (1977)
Kleckner, N.: Translocatable elements in procaryotes. Cell 11, 11–23 (1977)
Kopecko, D.J., Brevet, J., Cohen, S.N.: Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J. Mol. Biol. 108, 333–360 (1976)
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)
Lovett, M.A., Katz, L., Helinski, D.R.: Unidirectional replication of plasmid ColE1 DNA. Nature 251, 337–340 (1974)
Matsuhashi, Y., Yagisawa, M., Kondo, S., Takeuchi, T., Umezawa, H.: Aminoglycoside 3′-phosphotransferase I and II in Pseudomonas aeruginosa. J. Antibiot. (Tokyo) 28, 442–447 (1975)
Meagher, R.B., Tait, R.C., Betlach, M., Boyer, H.W.: Protein expression in E. coli minicells by recombinant plasmids. Cell 10, 521–536 (1977)
Nomura, N., Yamagishi, H., Oka, A.: Isolation and characterization of transducing coliphage fd carrying a kanamycin resistance gene. Gene 3, 39–51 (1978)
Oka, A., Nomura, N., Sugimoto, K., Sugisaki, H., Takanami, M.: Nucleotide sequence at the insertion sites of a kanamycin transposon Nature 276, 845–847 (1978)
Philippsen, P., Thomas, M., Kramer, R.A., Davis, R.W.: Unique arrangement of coding sequences for 5S, 5.8S, 18S and 25S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis. J. Mol. Biol 123, 387–404 (1978)
Tait, R.C., Rodriguez, R.L., Boyer, H.W.: Altered tetracycline resistance in pSC101 recombinant plasmids. Mol. Gen. Genet. 151, 327–331 (1977)
Tanaka, T., Weisblum, B.: Construction of a colicin E1-R factor composite plasmid in vitro: Means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)
Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonuclease. J. Mol. Biol. 91, 315–328 (1975)
Timmis, K.N., Cabello, F., Cohen, S.N.: Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol. Gen. Genet. 162, 121–137 (1978)
Tomizawa, J., Sakakibara, Y., Kakefuda, T.: Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc. Natl. Acad. Sci. U.S.A. 71, 2260–2264 (1974)
Widera, G., Gautier, F., Lindenmaier, W., Collins, J.: The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR322. Mol. Gen. Genet. 163, 301–305 (1978)
Yamagishi, H., Inokuchi, H., Ozeki, H.: Excision and duplication of su3+-transducing fragments carried by bacteriophage Φ80 I. Novel structure of Φ80sus 2psu3+ DNA molecule. J. Virol. 18, 1016–1023 (1976)
Yamamoto, T., Fujii, A., Yokota, T.: A multiply temperaturesensitive kanamycin resistance factor and its spontaneous mutants relating to temperature sensitivity. In: Microbial drug resistance (S. Mitsuhashi and H. Hashimoto, eds.), pp. 125–133. Tokyo: University of Tokyo Press 1975
Yamamoto, T., Yokota, T.: Host-dependent, thermosensitive replication of an R plasmid, pJY5, isolated from Enterobacter cloacae. J. Bacteriol. 132, 923–930 (1977)
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Yamamoto, T., Yokota, T. Construction of a physical map of a kanamycin (Km) transposon, Tn5, and a comparison to another km transposon, Tn903. Molec. Gen. Genet. 178, 77–83 (1980). https://doi.org/10.1007/BF00267215
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DOI: https://doi.org/10.1007/BF00267215