Summary
In order to localize the genes coding for zein, the major storage protein of maize endosperm, zein 125I-mRNA and 3H-cDNA labelled at high specific activity were used for in situ hybridization on heterozygous interchanges and paracentric inversions of the KYS strain of Zea mays. The analysis of the diplotene-metaphase I microsporocytes indicated the presence of zein structural genes on the long arm of chromosomes 4 and 5, the short arm of chromosome 7 and the distal segment of the long arm of chromosome 10. The two hybridization sites on chromosomes 7 and 10 are found near opaque-2 and opaque-7 loci which are known to regulate zein synthesis. The present data are discussed in relation to results obtained by other authors using genetical mapping of zein genes.
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Communicated by H. Stubbe
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Viotti, A., Pogna, N.E., Balducci, C. et al. Chromosomal localization of zein genes by in situ hybridization in Zea mays . Molec. Gen. Genet. 178, 35–41 (1980). https://doi.org/10.1007/BF00267210
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DOI: https://doi.org/10.1007/BF00267210