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Extranuclear recombination in Aspergillus nidulans: Closely-linked multiple chloramphenicol- and oligomycin-resistance loci

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Summary

A nuclear, chloramphenicol-sensitive mutant cas-1 has been isolated which is cross sensitive to a number of drugs, including oligomycin and cycloheximide. Approximately one-third of the chloramphenicol-resistant mutants isolated from mutagenized conidia of this strain were found to be extranuclear, and exhibited a variety of phenotypes. One of these mutants, designated (camB51), was slow growing on drug-free medium and recombined at low frequency with the previously described mutant (camA112) (Gunatilleke et al., 1975).

The majority of extranuclear oligomycin-resistant mutants isolated from cas-1 were indistinguishable from (oliA1) (Rowlands and Turner, 1973). Two mutants, (oliB322) and (oliB332), with similar but not identical phenotypes to (oliA1), recombined with the latter at low frequency but not with each other, thus representing a new class of extranuclear mutants.

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References

  • Alderson, T., Hartley, M.J.: Specificity for spontaneous and induced mutation at several gene loci in Aspergillus nidulanss. Mutation Res. 8, 255–264 (1969)

    Google Scholar 

  • Avner, P.R., Coen, D., Dujon, B., Slonimski, P.P.: Mitochondrial genetics IV. Allelism and mapping studies of oligomycin-resistant mutants in S. cerevisiae. Molec. gen. Genet. 125, 9–52 (1973)

    Google Scholar 

  • Avner, P.R., Griffiths, D.E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 312–321 (1973)

    Google Scholar 

  • Beale, G.H.: Genetic studies on mitochondrially inherited mikamycin-resistance in Paramecium aurelia Molec. gen. Genet. 127, 241–248 (1973)

    Google Scholar 

  • Beale, G.H., Knowles, J.K.C., Tait, A.: Mitochondrial genetics in Paramecium. Nature (Lond.) 235, 396–397 (1972)

    Google Scholar 

  • Coen. D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P.P.: Mitochondrial genetics I. Methodology and phenomenology. In: Control of organelle development Symp. Soc. exp. Biol. 24, 449–496 (1970)

  • Diacumakos, E.G., Garnjobst, L., Tatum, E.L.: A cytoplasmic character in Neurospora crassa. The role of nuclei and mitochondria. J. Cell Biol. 26, 427–443 (1965)

    Google Scholar 

  • Faye, G., Kujawa, C., Fukuhara, H.: Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of Saccharomyces cerevisiae J. molec. Biol. 88, 185–203 (1974)

    Google Scholar 

  • Garnjobst, L., Wilson, J.F., Tatum, E.L.: Studies on a cytoplasmic character in Neurospora crassa J. Cell Biol. 26, 413–425 (1965)

    Google Scholar 

  • Grivell, L.A., Netter, P., Borst, P., Slonimski, P.P.: Mitochondrial antibiotic resistance in yeast: ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin. Biochim. biophys. Acta (Amst.) 312, 358–367 (1973)

    Google Scholar 

  • Gunatilleke, I.A.U.N., Scazzocchio, C., Arst, Jr., H.N.: Cytoplasmic and nuclear mutations to chloramphenicol resistance in Aspergillus nidulans. Molec. gen. Genet. 137, 269–276 (1975)

    Google Scholar 

  • Heyting, C., Sanders, J.P.M.: The physical mapping of some genetic markers in the 21S ribosomal region of the mitochondrial DNA of yeast. In: The genetic function of mitochondrial DNA (eds. Saccone, C., Kroon, A.M.), pp. 273–280. Proceedings of the 10th International Bari Conference on mitochondria. Riva dei Tessali. Amsterdam: North-Holland Press 1976.

    Google Scholar 

  • Jinks, J.L.: Cytoplasmic inheritance in fungi. In: methodology in basic genetics (ed. Burdette, W.J.), pp. 325–354. San Francisco: Holden-Day Inc. 1963

    Google Scholar 

  • Kotylak, Z., Slonimski, P.P.: Joint control of cytochromes a and b by a unique mitochondrial DNA region comprising four genetic loci. In: The genetic function of mitochondrial DNA (eds. Saccone, C., Kroon, A.M.), pp. 143–154. Procedings of the 10th International Bari Conference on mitochondria. Riva dei Tessali. Amsterdam: North-Holland Press 1976

    Google Scholar 

  • Lancashire, W.E., Griffiths, D.E.: Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants. Europ. J. Biochem. 51, 403–413 (1975)

    Google Scholar 

  • Lazarus, C.M., Turner, G.: Novel extranuclear chloramphenicolresistant phenotypes in Aspergillus nidulans. Heredity 37, 150–151 (1976)

    Google Scholar 

  • López Pérez, M.J., Turner, G.: Mitochondrial DNA from Aspergillus nidulans. FEBS Letters 58, 159–163 (1975)

    Google Scholar 

  • Lusena, C.V., James, A.P.: Alterations in mitochondrial DNA of yeast which accompany genetically and environmentally controlled changes in p -mutability. Molec. gen. Genet. 144, 119–125 (1976)

    Google Scholar 

  • Mason, J.R., Turner, G.: Transmission and recombination of extranuclear genes during sexual crosses in Aspergillus nidulans. Molec. gen. Genet. 143, 93–99 (1975)

    Google Scholar 

  • Putrament, A., Baranowska, H., Ejchart, A., Prazmo, W.: Manganese mutagenesis in yeast. A practical application of manganese for the induction of mitochondrial antibiotic-resistant mutations. J. gen. Microbiol. 90, 265–270 (1975)

    Google Scholar 

  • Rosenberg, E., Mora, C., Edwards, D.L.: Selection of extranuclear mutants of Neurospora crassa. Genetics, 83, 11–24 (1976)

    Google Scholar 

  • Rowlands, R.T.: Extranuclear mutants of Aspergillus nidulans. Ph. D. Thesis, University of Bristol, England (1974)

  • Rowlands, R.T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Molec. gen. Genet. 126, 201–216 (1973)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Three marker mitochondrial crosses in Aspergillus nidulans. Molec. gen. Genet. 141, 69–79 (1975)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Nuclear-extranuclear interactions affecting oligomycin resistance in Aspergillus nidulans. Molec. gen. Genet. 154, 311–318 (1977)

    Google Scholar 

  • Shannon, C., Enns, R., Wheelis, L., Burchiel, K., Criddle, R.S.: Alterations in mitochondrial adenosine triphosphatase activity resulting from mutation of mitochondrial deoxyribonucleic acid. J. biol. Chem. 248, 3004–3011 (1973)

    Google Scholar 

  • Sriprakash, K.S., Choo, K.B., Nagley, P., Linnane, A.W.: Physical mapping of mitochondrial rRNA genes in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 69, 85–91 (1976)

    Google Scholar 

  • Trembath, M.K., Bunn, C.L., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria. 27. Genetic and biochemical characterisation of cytoplasmic and nuclear mutations to spiramycin resistance in Saccharomyces cerevisiae Molec. gen. Genet. 121, 35–48 (1973)

    Google Scholar 

  • Trembath, M.K., Molloy, P.L., Sriprakash, K.S., Cutting, G.J., Linnane, A.W., Lukins, H.B.: Biogenesis of mitochondria. 44. Comparative studies and mapping of mitochondrial oligomycin resistance mutations in yeast based on gene recombination and petite deletion analysis. Molec. gen. Genet. 145 43–52 (1976)

    Google Scholar 

  • Turner, G., Rowlands, R.T.: Cytochrome abnormalities and cyanide-resistant respiration in extranuclear mutants of Aspergillus nidulans. J. Bact. 125, 389–397 (1976)

    Google Scholar 

  • Turner, G., Watson, C.M.J.: A new extranuclear oligomycin-resistance locus in Aspergillus nidulans. Heredity 37, 151 (1976)

    Google Scholar 

  • Waldron, C., Roberts, C.F.: Cytoplasmic inheritance of a coldsensitive mutant in Aspergillus nidulans. J. gen. Microbiol. 78, 379–381 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Department of Bacteriology, The Medical School, University of Bristol, Bristol, England

    Colin M. Lazarus & Geoffrey Turner

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  1. Colin M. Lazarus
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  2. Geoffrey Turner
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Communicated by F. Kaudewitz

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Lazarus, C.M., Turner, G. Extranuclear recombination in Aspergillus nidulans: Closely-linked multiple chloramphenicol- and oligomycin-resistance loci. Molec. Gen. Genet. 156, 303–311 (1977). https://doi.org/10.1007/BF00267186

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  • DOI: https://doi.org/10.1007/BF00267186

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