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Genetic analysis of the dnaB region of Escherichia coli K-12: The identification of a recombinational hot spot

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Summary

The insertion of an F′ into the malB-dnaB-ampA region of Escherichia coli K-12 was examined. It was found that insertion occurred preferentially at a site within the dnaB gene. The presence of mutations in this gene did not seem to alter the site of F′ insertion but in some cases did affect the frequency at which this recombinational event took place. The map position of various dnaB alleles relative to this site was determined and compared with the allele order obtained by P1 transduction. Models to explain the nonrandom pattern of insertion are discussed.

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  1. Imperial Cancer Research Fund, Burtonhole Lane, NW7 1AD, London, England

    Paul F. Schendel

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  1. Paul F. Schendel
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Communicated by F. Stahl

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Schendel, P.F. Genetic analysis of the dnaB region of Escherichia coli K-12: The identification of a recombinational hot spot. Molec. Gen. Genet. 156, 281–287 (1977). https://doi.org/10.1007/BF00267183

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  • DOI: https://doi.org/10.1007/BF00267183

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