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On the dose response in B. subtilis transfection

Involvement of aggregates in ϕ29 transfection

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Summary

Transfection with ϕ29 DNA is predominantly caused by multimolecular, protease-sensitive aggregates of DNA. A minority of transfecting DNA molecules having properties of unit-length ϕ29 genome molecules show a quadratic dose response in transfection.

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Author notes

  1. H. Hirokawa

    Present address: Office for Life Science Promotion, The Institute of Physical and Chemical Research, 2-28-8 Honkomagome, Bunkyo-Ku, 113, Tokyo, Japan

Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestrasse 63/73, D-1000, Berlin 33

    H. Hirokawa, T. A. Trautner & G. Lüder

Authors
  1. H. Hirokawa
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  2. T. A. Trautner
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  3. G. Lüder
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Communicated by E. Bautz

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Hirokawa, H., Trautner, T.A. & Lüder, G. On the dose response in B. subtilis transfection. Molec. Gen. Genet. 156, 263–266 (1977). https://doi.org/10.1007/BF00267180

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  • DOI: https://doi.org/10.1007/BF00267180

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