Summary
Eleven cistrons of genes active in lytic growth of phage P22 were classified by phenotypic expression of their amber mutants in nonpermissive Salmonella typhimurium. Seven cistrons code for late functions according to their lysis positive phenotype. Of the remaining four cistrons one codes for lysozym synthesis, two for phage directed DNA synthesis and one seems to be engaged in regulation of late gene expression.
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Botstein, D., Chan, R. K., Weddell, C. H.: Genetics of bacteriophage P22 II. Gene order and gene function. Virology 49, 268–282 (1972)
Boyd, J. S. K., Bidwell, D. E.: The Q1(A) strains of Salmonella typhimurium: Induction phenomena. J. gen. Microbiol. 21, 635–651 (1959)
Bronson, M. J., Levine, M.: Virulemt mutants of bacteriophage P22 I. Isolation and genetic analysis. J. Virol. 7, 559–568 (1971)
Bronson, M. J., Levine, M.: II. Physiological analysis of P22 vir B-3 and its component mutations. Virology 47, 644–655 (1972)
Dopatka, H. D.: Charakterisierung von amber des Mutanten Salmonella-Bakteriophagen P22. Dissertation Göttingen (1971)
Dopatka, H. D., Prell, H. H.: Amber mutants of Salmonella-phage P22 in genes engaged in the establishment of lysogeny. Molec. gen. Genet. 120, 157–170 (1973)
Gough, M., Levine, M.: The circularity of the phage P22 linkage map. Genetics 58, 161–169 (1968)
Kolstad, R., Prell, H. H.: An amber-map of Salmonella phage P22. Molec. gen. Genet. 144, 339–350 (1969)
Levine, M., Schott, C.: Mutations of phage P22 affecting phage DNA synthesis and lysogenization. J. molec. Biol. 62, 53–64 (1971)
Mark, K.-K.: The relationship between the synthesis of DNA and the synthesis of phage lysozyme in E. coli infected by bacteriophage T4. Virology 42, 20–27 (1970)
Smith, H. O., Levine, M.: The synthesis of phage and host DNA in the establishment of lysogeny. Virology 25, 585–590 (1965)
Tsugita, A., Inouye, M., Terzaghi, E., Streisinger, G.: Purification of bacteriophage T4 lysozyme. J. biol. Chem. 243, 391–397 (1968)
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Communicated by E. Bautz
The majority of the experiments presented are a part of the dissertation of H. D. Dopatka submitted 1971 to the University of Göttingen.
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Bode, W., Dopatka, H.D. & Prell, H.H. Functional classification of P22 Amber mutants. Molec. Gen. Genet. 127, 341–347 (1973). https://doi.org/10.1007/BF00267104
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DOI: https://doi.org/10.1007/BF00267104