Summary
Treatment of E. coli B bacteria by the base analogue 2-aminopurine, before infection with T4 phages, induces mutations of the transition type into the virus. Treatment of the phage-bacterium complex, only during the first four minutes of the latent period i.e. at a moment where no phage DNA is synthetized, is also mutagenic. The kinetics of acquisition and loss by treated bacteria of mutagenicity and the action of various metabolic inhibitors show that the base analogue is stored into the bacterial or the phage messenger RNA from which it is reused to be incorporated into the phage DNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Champe, S. P., Benzer, S.: Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger RNA. Proc. nat. Acad. Sci. (Wash.) 48, 532–546 (1962)
Fermi, G., Stent, G. S.: Effect of chloramphenicol and of the multiplicity of infection on induced mutation in bacteriophage T4. Z. Vererbungsl. 93, 177–187 (1962)
Frankel, F. R.: The absence of mature phage DNA molecules from the replicating pool of T-even infected Escherichia coli. J. molec. Biol. 18, 109–126 (1966)
Freese, E.: The specific mutagenic effect of base analogues on phage T4. J. molec. Biol. 1, 87–105 (1959)
Gottschling, H., Freese, E.: Incorporation of 2-aminopurine into the deoxyribonucleic acid of bacteria and bacteriophages. Z. Naturforsch. 16b, 515–519 (1961)
Hershey, A. D., Garen, A., Fraser, D. K., Hundis, J. D.: Growth and inheritance in bacteriophage. Carnegie Inst. Wash. Yearbook 53,210–225 (1954)
Kozloff, L. M.: Origin and fate of bacteriophage material. Cold Spr. Harb. Symp. quant. Biol. 18, 209–220 (1953)
Lazzarini, R. A., Dahlberg, A. E.: The control of ribonucleic acid synthesis during aminoacid deprivation in Escherichia coli. J. biol. Chem. 246, 420–429 (1971)
Lazzarini, R. A., Winslow, R. M.: The regulation of RNA synthesis during growth rate transitions and aminoacid deprivation in Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 35, 383–390 (1970)
Lindahl, L., Forschhammer, J.: Evidence for reduced breakdown of messenger RNA during blocked transcription or translation in Escherichia coli. J. molec. Biol. 43, 593–606 (1969)
Marcovich, H., Vigier, P. R. R.: Induction de mutations chez le bacteriophage T4 par un traitement des bactéries avec de la 2-aminopurine avant l'infection.C.R. Acad. Sci. (Paris) 274, 1860–1863 (1972)
Morse, D. E.: Delayed-early mRNA for the tryptophan operon. An effect of chloramphenicol. Cold Spr. Harb. Symp. quant. Biol. 35, 495–496 (1970)
Moyed, H. S.: False feedback inhibition; Inhibition of tryptophan synthesis by 5-methyl-tryptophan. J. biol. Chem. 235, 1098–1102 (1960)
Nierlich, D. P.: Aminoacid control over RNA synthesis: a reevaluation. Proc. nat. Acad. Sci. (Wash.) 60, 1345–1352 (1968)
Rogan, E. G., Bessman, M. J.: Studies on the pathway of incorporation of 2-aminopurine into the deoxyribonucleic acid of Escherichia coli. J. Bact. 103, 622–633 (1970)
Rudner, R.: Mutations as an error in base pairing. I. — The mutagenicity of base analogues and their incorporation into the DNA of Salmonella typhimurium. Z. Verebungsl. 92, 336–360 (1961)
Speyer, J., Karam, J. D., Lenny, A. B.: On the role of DNA polymerases in base selection. Cold Spr. Harb. Symp. quant. Biol. 31, 693–697 (1966)
Stent, G. S., Maale, O.: Radioactive phosphorus tracer studies in the reproduction of T4 bacteriophage II. — Kinetics of phosphorus assimilation. Biochim. biophys. Acta (Amst.) 10, 55–69 (1953)
Stern, J. L., Sekigushi, M., Barner, H. D., Cohen, S. S.: The synthesis of messenger RNA without protein synthesis I. — Studies with thymineless strains of Escherichia coli. J. molec. Biol. 8, 629–637 (1964)
Strelzoff, E.: Identification of base pairs, involved in mutations induced by base analogues. Biochem. biophys. Res. Commun. 5, 384–388 (1961)
Wacker, A., Kirschfeld. S., Trager, L.: Über den Einbau Purin-analoger Verbindungen in die Bakterien-Nukleinsäure. J. molec. Biol. 2, 241–242 (1960)
Wacker, A., Loderman, E., Gauri, K., Chandra, P.: Synthesis and coding properties of 2-aminopurine polyribonucleotides. J. molec. Biol. 18, 382–383 (1966)
Warner, H. R., Hobbs, M. B.: Effect of hydroxyurea, on the replication of bacteriophage T4 in Escherichia coli. J. Virol. 3, 331–336 (1969)
Warren, R. J., Bose, S. K.: Bacteriophage induced inhibition of host functions. I. — Degradation of Escherichia coli deoxyribonucleic acid after T4 infection. J. Virol. 2, 327–334 (1968)
Weed, L. L., Cohen, S. S.: Utilization of host pyrimidines in the synthesis of bacterial viruses/ J. biol. Chem. 192, 693–700 (1951)
Young, I. E. T.: Control of functionnal T4 messenger synthesis. Cold Spr. Harb. Symp. quant. Biol. 35, 189–195 (1970)
Author information
Authors and Affiliations
Additional information
Communicated by F. Gros
This investigation was supported by the Centre National de la Recherche Scientifique (L. A. No 86 et 136), l'Institut Pasteur, la Délégation Générale à la Recherche Scientifique and la Fondation pour la Recherche Medicale.
Rights and permissions
About this article
Cite this article
Vigier, P.R.R., Marcovich, H. 2-Aminopurine induced mutations in T4 bacteriophage. Molec. Gen. Genet. 127, 317–325 (1973). https://doi.org/10.1007/BF00267102
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267102