Summary
Bacteriophage ΦX174 replicative from DNA (RF DNA) was formed in the presence of chloramphenicol at a concentration of 40 μg per ml and isolated at 12 and at 55 min. after infection. The component I RF DNA (double stranded covalently closed and twisted form) was separated and divided into a monomer and multimer (dimer) fraction.
The frequency of recombinants found after phage formation in the chloramphenicol treated cells and that found after spheroplast infection with the monomer molecules both increase with the time of RF formation. However, the frequency of recombinant molecules among the dimers remained constant. This finding is explained by the hypothesis that two separate mechanisms act in ΦX174 recombination, one of which is restricted to the formation of dimers.
Irradiation with UV of phage prior to infection showed that the frequency of recombinants in monomers increased, as the recombination frequency of phage after (a single) growth (step) did, but that neither the frequency of recombinant molecules in dimers is raised, nor the frequency of dimers. Using a recombination negative host the frequency of recombinant dimer molecules was three to fourfold decreased, whereas the frequency of dimers was only slightly lower (relative to the normal host). These results support the hypothesis mentioned above and moreover lend support to the view that the greater part of the dimers is not formed by recombination events.
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Hofs, E.B.H., van de Pol, J.H., van Arkel, G.A. et al. Dimeric circular duplex DNA of bacteriophage ΦX174 and recombination. Molec. Gen. Genet. 118, 161–172 (1972). https://doi.org/10.1007/BF00267085
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DOI: https://doi.org/10.1007/BF00267085