Summary
At least eight methF55 suppressor loci have been identified (Tables 1-4). All suppressors tested were effective towards methA, methB and methG mutants, while some of them were effective also towards methD10, methH2 and probably methE53 mutants (Tables 5 and 6).
In the progenies of non-leaky methionine mutants leaky segregants were found (Table 7).
Double mutant strains carrying methE31 and methA34 or methF55 or methD10 mutants reverted with approximately the same frequencies as did strains carrying a single methE31 mutant (Table 8).
The possible implications of these findings, together with the other data on methionine mutants and their suppressors in A. nidulans, are discussed.
Similar content being viewed by others
References
Alderson, T., Clark, A. M.: Interlocus specificity for chemical mutagens in Aspergillus nidulans. Nature (Lond.) 210, 593–595 (1966).
Ayling, P. D.: Methionine suppressors in Aspergillus nidulans: their genetics and behaviour in heterokaryons and diploids. Genet. Res. Camb. 14, 275–289 (1969).
Dorn, G. L.: A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56, 619–631 (1967).
Gajewski, W., Litwinska, J.: Methionine loci and their suppressors in Aspergillus nidulans. Molec. Gen. Genetics 102, 210–220 (1968).
Klimczuk, J.: Ph. D. Thesis, Warsaw University (1968).
Lhoas, P.: Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature (Lond.) 190, 774 (1961).
Lilly, L. J.: An investigation of the suitability of the suppressors of meth1 in Aspergillus nidulans for the study of induced and spontaneous mutation. Mutation Res. 2, 192–195 (1965).
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D., Bufton, A. W. J.: The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238 (1953).
Sherman, F.: Mutants of yeast deficient in cytochrome c. Genetics 49, 39–48 (1964).
—, Stewart, J. F., Margoliash, E., Parker, J., Campbell, W.: The structural gene of yeast cytochrome c. Proc. nat. Acad. Sci. (Wash.) 55, 1498–1504 (1966).
Siddiqi, O. H.: Mutagenic action of nitrous acid on Aspergillus nidulans. Genet. Res. Camb. 3, 303–314 (1962).
Weglenski, P.: Genetical analysis of proline mutants and their suppressors in Aspergillus nidulans. Genet. Res. Camb. 8, 311–321 (1966).
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Putrament, A., Guzewska, J. & Pieniazek, D. Further genetic characteristics of methionine mutants and their suppressors in Aspergillus nidulans . Molec. Gen. Genet. 109, 209–218 (1970). https://doi.org/10.1007/BF00267009
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267009