Summary
Triploid fish were obtained using heat-shock treatment. The optimal conditions for the heat shock (39.5±0.2°C for 3.5–4 min) as well as the exact zygote age (3 min) at which this heat shock was applied were studied. Results showed that this treatment gives rise to 100% of triploid fish with a satisfactory survival rate of 61% beyond the yolk sac resorption. The genital papillae of this triploid fish were underdeveloped in comparison to normal diploid fish. However, no morphological or growth-rate differences between diploid and triploid fish could be observed up to the age of 6 months. Triploidy was assessed by the karyotyping of embryo cells or adult PHA-stimulated lymphocytes, or by erythrocyte measurements. The occurrence of a heat-shock sensitive event at the zygotic age of 6 min is discussed.
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Communicated by E. J. Eisen
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Don, J., Avtalion, R.R. The induction of triploidy in Oreochromis aureus by heat shock. Theoret. Appl. Genetics 72, 186–192 (1986). https://doi.org/10.1007/BF00266991
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DOI: https://doi.org/10.1007/BF00266991