Summary
Taking advantage of the Spi (Sensitivity to P2 interference) phenomenon, bacterial mutants seemingly resistant to phage λsusN7nin5, but sensitive to phage λspi, were isolated from a strain of E. coli K12 carrying no nonsense suppressor and lysogenic for P2. A class of these mutants, designated nitA (N-independent transcription), is described here.
Upon infection of the nitA mutants with a trp transducing phage λsusN7N53ptrp46 which carries the E. coli trpE and D genes in the CIII-att region of the λ genome, formation of anthranilate synthetase (ASase, a complex protein of trp E and D gene products) was clearly demonstrated. In contrast, no ASase formation was observed in the parent nitA +strain under the same conditions. The synthesis is subject to “turn off” control, and is completely repressed by the CI repressor of phage λ. The nitA cells lysogenic for λCI857susN7N53 are killed by thermal induction much more efficiently than the parent cells lysogenic for the same phage. The nitA mutants support the growth of λsusN7N53byp much better than the parent. These results suggest that the nitA mutation permits the early leftward and rightward transcription of the λ genome in the absence of the N gene product.
On the E. coli genetic map, nitA is located between ilv and metE, nearer to ilv. The mutant allele is recessive to the wild-type allele. The present evidence, together with results of biochemical investigations to be reported, suggests that nitA is a gene specifying the transcription termination factor rho.
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References
Beckwith, J.: Restoration of operon activity by suppressor. Biochim. biophys. Acta (Amst.) 76, 162 (1963)
Bertani, G.: Lysogeny. Advanc. Virus Res. 5, 151 (1958)
Brachet, P., Eisen, H., Rambach, A.: Mutation of coliphage λ affecting the expression of replicative functions O and P. Molec. gen. Genet. 108, 266 (1970)
Butler, B., Echols, H.: Regulation of bacteriophage λ development by gene N: properties of a mutation that bypass N control of late protein synthesis. Virology 40, 212 (1970)
Calendar, R.: The regulation of phage development. Ann. Rev. Microbiol. 24, 242 (1970)
Calendar, R., Lindqvist, B., Sironi, G., Clark, A.J.: Characterization of REP- mutants and their interaction with P2 phage. Virology 40, 72 (1970)
Chadwick P., Pirrotta, V., Steinberg, R., Hopkins, N., Ptashne, M.: The λ and 434 phage repressors. Cold Spr. Harb. Symp. quant. Biol. 35, 283 (1970)
Cohen, S.N., Chang, A.C.Y.: Genetic expression in bacteriophage λ IV Effects of P2 prophage on λ inhibition of host synthesis and λ gene expression. Virology 46, 397 (1971)
Court, D., Sato, K.: Studies of novel transducing variants of lambda: dispensability of genes N and Q Virology 39, 348 (1969)
Echols, H.: Developmental pathways for the temperate phage: lysis vs. lysogeny. Ann. Rev. Genet. 6, 157 (1972)
Fiandt, M., Hradecna, Z., Lozeron, H.A., Szybalski, W.: Electron micrographic mapping of deletions, insertions, inversions and homologies in the DNAs of coliphages lambda and phi 80. The bacteriophage lambda (A. Hershey, ed.), p. 329. New York: Cold Spring Harbor Laboratory 1971
Franklin, N.C.: The N operon of λ: Extent and regulation as observed in fusions to the tryptophan operon of E. coli. The bacteriophage lambda (A. Hershey, ed.), p. 621. New York: Cold Spring Harbor Laboratory 1971
Franklin, N.C.: Altered reading of genetic signals fused to the N operon of bacteriophage λ: Genetic evidence for modification of polymerase by the protein product of the N gene. J. molec. Biol. 89, 33 (1974)
Friedman, D.I., Jolly, C.T., Mural, R.J.: Interference with the expression of the N gene function of phage λ in a mutant of Escherichia coli Virology 51, 216 (1973)
Georgopoulos, C.P.: Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc. nat. Acad. Sci. (Wash.) 68, 2977 (1971)
Ghysen, A., Pironio, M.: Relationship between the N function of bacteriophage λ and host RNA polymerase. J. molec. Biol. 65, 259 (1972)
Heineman, S.F., Spiegelman, W. G.: Role of the gene N product in phage lambda. Cold Spr. Harb. Symp. quant. Biol. 35, 315 (1970)
Herskowitz, I.: Control of gene expression in bacteriophage lambda. Ann. Rev. Genet. 7, 289 (1973)
Hiraga, S.: Operator mutants of the tryptophan operon in Escherichia coli. J. molec. Biol. 39, 159 (1969)
Inoko, H., Imai, M.: Involvement of some host function of Escherichia coli in the turn off control after infection with phages ϕ80 and λ. Molec. gen. Genet. 129, 49 (1974)
Inoko, H., Naito, S., Ito, K., Imai, M.: Regulation of the tryptophan operon of Escherichia coli integrated into the phage ϕ80 genome. Molec. gan. Genet. 129, 37 (1974)
Kaiser, D.: Lambda DNA replication. The bacteriophage lambda (A. Hershey, ed.), p. 195. New York:Cold Spring Harbor Laboratory 1971
Koga, H., Horiuchi, T.: A new repressor produced by λCIvirCR mutant. Jap. J. Genet. 46, 285 (1971)
Kumar, S., Calef, E., Szybalski, W.: Regulation of the transcription of Escherichia coli phage λ by its early genes N and tof. Cold Spr. Harb. Symp. quant. Biol. 35, 331 (1971)
Kuwano, M., Schlessinger, D., Morse, D.E.: Loss of dispensable endonuclease activity in relief of polarity by suA. Nature (Lond.) New Biol 231, 214 (1971)
Lederberg, J.: Methods in medical research (Comroe, J.H. Jr. ed.), vol. 3, p. 3. Year Book Publishers, Chicago 1950
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190 (1955)
Lieb, M.: λ mutants that persist as plasmids. J. Virol. 6, 218 (1970)
Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage lambda: Abortive infection of bacteria lysogenic for phage P2. Proc. nat. Acad. Sci. (Wash.) 66, 587 (1970)
Lowery-Goldhammer, C., Richardson, J.P.: An RNA-dependent nucleoside triphosphate phosphohydrolase (ATPase) associated with rho termination factor. Proc. nat. Acad. Sci. (Wash.) 71, 2003 (1974)
Luzzati, D.: Regulation of λ exonuclease synthesis: Role of the N gene product and λ repressor. J. molec. Biol. 49, 515 (1970)
Mark, K.: Effect of the N-bypass mutations nin and byp on the rightward transcription in coliphage lambda. Molec. gen. Genet. 124, 291 (1973)
Matsubara, K.: Interference in phage growth by a resident plasmid λdv. I. The mode of interference. Virology 58, 713 (1972)
Morse, D.E., Primakoff, P.: Relief of polarity in E. coli by “suA”. Nature (Lond.) 226, 28 (1970)
Ogawa, T., Tomizawa, J.: Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions. J. molec. Biol. 38, 217 (1968)
Oppenheim, A., Honigman, A., Oppenheim, A.B.: Interference with phage lambda cro gene function by a colicin-tolerant Escherichia coli mutan. Virology 61, 1 (1974)
Pereira da Silva, L.H., Eisen, H.A., Jacob, F.: Sur la réplication du bactériophage λ. C.R.Acad.Sci. (Paris) 266, 926 (1968)
Pereira da Silva, L.H., Jacob, F.: Erude génétique d'une mutation modifiant la sensibilité à l'immunité chez le bacteriophage lambda. Ann. Inst. Pasteur 115, 145 (1968)
Pero, J.: Location of the phage λ gene responsible for turning off λ-exonuclease synthesis. Virology 40, 65 (1970)
Pero, J.: Deletion mapping of the site of action of the tof gene product. The bacteriophage lambda (A. Hershey, ed.), p. 599. New York: Cold Spring Harbor Laboratory 1971
Portier, M.M., Marcaud, L., Cohen, A., Gros, F.: Mechanism of transcription of the N operon of bacteriophage lambda. Molec. gen. Genet. 17, 72 (1972)
Roberts, J.W.: Termination factor for RNA synthesis. Nature (Lond.) 224, 1168 (1969)
Roberts, J.W.: The ϱ factor: Termination and antitermination in λ. Cold Sprl Harb. Symp. quqnt. Biol. 35, 121 (1970)
Signer, E.R.: Plasmid formation: A new mode of lysogeny by bacteriophage λ. Nature (Lond.) 223, 158 (1969)
Sly, W.S., Eisen, H.A., Siminovitch, L.: Host survival following infection with or induction of bacteriophage lambda mutants. Virology 34, 112 (1968)
Sly, W.S., Rabideau, K., Kolber, A.: The mechanisms of lambda virulence II. Regulatory mutations in classical virulence. The bacteriophage lambda (A. Hershey, ed.), p. 575. New York: Cold Spring Harbor Laboratory 1971
Steinberg, R.A., Ptashne, M.: In vitro repression of RNA synthesis by purified λ phage repressor. Nature (Lond.) New Biol. 230, 76 (1971)
Szybalski, W., Bøvre, K., Fiandt, M., Hayes, S., Hradecna, Z., Kumar, S., Lozeron, H.A., Nijkamp, H.J., Stevens, W.F.: Transcriptional units and their controls in Escherichia coli phage λ: Operons and scriptons. Cold Spr. Harb. Symp. quant. Biol. 35, 341 (1970)
Taylor, A.L., Trotter, C.D.: Linkage map of Escherichia coli K12. Bact. Rev. 36, 504 (1972)
Vogel, H.J., Bonner, D.M.: Acetylornithinase of Escherichia coli: Partial purification and some properties. J. biol. Chem. 218, 97 (1956)
Yamamoto, K., Alberts, B.M., Benzinger, R., Lawhorne, L., Treiber, G.: Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40, 734 (1970)
Yokota, T., Gots, J.S.: Requirement of adenosine 3′5′-cyclic monophosphate for flagella formation in Escherichia coli and Salmonella typhimurium. J. Bact. 103, 513 (1970)
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage lambda II. Inhibition of growth by prophage P2. The bacteriophage lambda (A. Hershey, ed.), p. 469. New York: Cold Spring Harbor Laboratory 1971
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Inoko, H., Imai, M. Isolation and genetic characterization of the nitA mutants of Escherichia coli affecting the termination factor rho. Molec. Gen. Genet. 143, 211–221 (1976). https://doi.org/10.1007/BF00266924
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DOI: https://doi.org/10.1007/BF00266924