Summary
Transducing λ bacteriophages have been isolated which carry the divergently transcribed argECBH operon of E. coli K12 and various portions of the adjacent ppc and bfe chromosomal regions. They were recovered from lysates prepared by the procedure of Schrenk and Weisberg using a Ppc+ Arg+ Bfe+ strain carrying a deletion of the usual attachment site of λ.
Heteroduplex DNA mapping of these λdarg and of the ϕ80 darg isolated by B. Konrad indicates that the two kinds of phages carry the arg cluster in opposite orientations, a situation favorable for the isolation of argECBH DNA.
A physical map of the ppc argECBH bfe region including 2 unusual attachment sites of λ has been constructed. The localization of the end points of certain arg deletions provides a useful reference framework for the currently pursued mapping of mutations affecting the control of divergent transcription and for the location of restriction enzyme cleavage sites in the arg region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyen, A., Cunin, R., Glansdorff, N.: Messenger RNA levels from the bipolar arginine ECBH operon of Escherichia coli Arch. int. Physiol. 82, 968–969 (1974)
Brack, Ch., Delain, E., Riou, G.: Replicating covalently closed, circular DNA from kinetoplasts of Trypanosoma cruzi. Proc. nat. Acad. Sci. (Wash.) 69, 1642–1646 (1972)
Bradley, D.E.: The preparation of specimen support films. In: Techniques for electron microscopy (D.E. Kay ed.), p. 58–65. Philadelphia. F. A. Davis Co. 1965
Buxton, R.S.: Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins. Molec. gen. Genet. 113, 154–156 (1971)
Cunin, R., Boyen, A., Pouwels, P., Glansdorff, N., Crabeel, M.: Parameters of gene expression in the bipolar argECBH operon of E. coli K12. The question of translational control. Molec. gen. Genet. 140, 51–60 (1975)
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (L. Grossman and K. Moldave eds.), vol. 21, p. 413–428. New York: Academic Press 1971
Elseviers, D., Cunin, R., Glansdorff, N., Baumberg, S., Ashcroft, E.: Control regions within the argECBH cluster of Escherichia coli K12. Molec. gen. Genet. 117, 349–366 (1972)
Glansdorff, N.: Topography of cotransducible arginine mutations in Escherichia coli K12. Genetics 51, 167–179 (1965)
Glansdorff, N.: Le contrôle génétique des biosynthèses de l'arginine et du carbamyl phosphate chez E. coli. Ph.D. Thesis, University of Brussels (1966)
Gorini, L., Kaufman, H.: Selecting bacterial mutants by the penicillin method. Science 131, 604–605 (1960)
Hershey, A.D., ed. The bacteriophage lambda. New York: The Cold Spring Harbor Laboratory 1970
Jacoby, G.A.: Control of the argECBH cluster in Escherichia coli. Molec. gen. Genet. 117, 337–348 (1972)
Kikuchi, A., Elseviers, D., Gorini, L.: Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes. J. Bact. 122, 727–742 (1975)
Kirschbaum, J.B., Konrad, E.B.: Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase. J. Bact. 116, 517–526 (1973)
Lang, D., Mitani, M.: Simplified quantitative electron microscopy of biopolymers. Biopolymers 9, 373–379 (1970)
Mergeay, M., Gigot, D., Beckmann, J., Glansdorff, N., Pierard, A.: Physiology and genetics of carbamoyl-phosphate synthesis in Escherichia coli K12. Molec. gen. Genet. 133, 299–316 (1974)
Miller, J.: Experiments in molecular genetics. New York: The Cold Spring Harbor Laboratory 1972
Ohtsubo, E., Lee, H.J., Deonier, R.C., Davidson, N.: Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli. VI. Mapping of F14 sequences homologous to ϕ80dmetBJF and ϕ80darg ECBH bacteriophages. J. molec. Biol. 89, 599–618 (1974)
Panchal, C.J., Bagchee, S.N., Guha, A.: Divergent orientation of transcription from the arginine gene ECBH cluster of Escherichia coli. J. Bact. 117, 675–680 (1974)
Pouwels, P.H., Cunin, R., Glandorff, N.: Divergent transcription in the argECBH cluster of genes in Escherichia coli K12. J. molec. Biol. 83, 421–424 (1974)
Press, R., Glansdorff, N., Miner, P., DeVries, J., Kadner, R., Maas, W.K.: Isolation of transducing particles of ϕ80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. nat. Acad. Sci. (Wash.). 68, 795–798 (1971)
Schrenk, W.J., Weisberg, R.A.: A simple method for making new transducing lines of coliphage λ. Molec. gen. Genet. 137, 101–107 (1975)
Shapiro, J., MacHattie, L., Eron, L., Ihler, G., Ippen, K., Beckwith, J.: Isolation of pure lac operon DNA. Nature (Lond.) 224, 768–774 (1969)
Sharp, P.A., Hsu, M.T., Ohtsubo, E., Davidson, N.: Electron microscope heteroduplex studies of sequences relations among plasmids of E. coli: I. Structure of F-prime factors. J. molec. Biol. 71, 471–497 (1972)
Shenk, T.E., Rhodes, C., Rigby, P.W.J., Berg, P.: Biochemical method for mapping mutational alterations in DNA with S1 nucleases: The location of deletions and temperature-sensitive mutations in simian virus 40. Proc. nat. Acad. Sci. (Wash.) 72, 989–993 (1975)
Shimada, K., Weisberg, R.A., Gottesman, M.E.: Prophage lambda at unusual chromosomal locations I. Location of the secondary attachment sites and the properties of the lysogens. J. molec. Biol. 63, 483–503 (1972)
Shimada, K., Weisberg, R.A., Gottesman, M.E.: Prophage lambda at unusual chromosomal locations III. The components of the secondary attachment sites. J. molec. Biol. 93, 415–429 (1975)
Taylor, A.L., Trotter, C.D.: Linkage map of Escherichia coli strain K12. Bact. Rev. 36, 504–524 (1972)
Vogel, H.J., McLellan, W.L.: N-acetyl-γ-glutamokinase (Escherichia coli). In: Methods in enzymology (H. Tabor and C.W. Taylor eds.), vol. 17 Part A, p. 251–255. New York: Academic Press 1970
Vogel, H.J., McLellan, W.L.: N-acetyl glutamic γ-semialdehyde dehydrogenase (Escherichia coli). In: Methods in enzymology (H. Tabor and C.W. Taylor eds.), vol. 17 Part A, p. 255–260. New York: Academic Press 1970
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Mazaitis, A.J., Palchaudhuri, S., Glansdorff, N. et al. Isolation and characterization of λdargECBH transducing phages and heteroduplex analysis of the argECBH cluster. Molec. Gen. Genet. 143, 185–196 (1976). https://doi.org/10.1007/BF00266921
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00266921