Summary
A cultivar lacking the glycinin subunit A5A4B3 (‘Raiden’) was crossed with one lacking the α′-subunit of β-conglycinin (‘Keburi’). Analysis of F2 and F3 progeny indicated that the missing bands of the A5A4B3 and the α′-subunit were each controlled by a recessive allele of two independently segregating genes. Gene symbols Gy 4/gy 4 and Cgy 1/cgy 1 were proposed for the genes which confer the presence or absence of the glycinin and conglycinin subunits, respectively.
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References
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Derbyshire E, Wright DJ, Boulter D (1976) Legumin and vicilin, storage proteins of legume seeds. Phytochemistry 15:3–24
Goldberg RB, Hoschek G, Tam SH, Ditta GS, Breibenbach RW (1981) Abundance, diversity, and regulation of mRNA sequence sets in soybean embryogenesis. Dev Biol 83:201–217
Kitamura K, Kaizuma N (1981) Mutant strains with low level of 7S globulin in soybean (Glycine max Merr.) seed. Jpn J Breed 31:353–359
Kitamura K, Toyokawa Y, Harada K (1980) Polymorphism of glycinin in soybean seeds. Phytochemistry 19:1841–1843
Larkins B (1983) Genetic engineering of seed storage proteins. In: Kosuge T, Meredith CP, Hollaender A (eds) Genetic engineering of plants. Plenum, New York, pp 93–118
Millerd A (1975) Biochemistry of legume seed proteins. Annu Rev Plant Physiol 26:53–72
Moreira MA, Hermodson MA, Larkins BA, Nielsen NC (1981) Comparison of the primary structure of the acidic polypeptides of glycinin. J Biol Chem 210:633–642
Nielsen NC (1984) Chemistry of legume storage proteins. Philos Trans R Soc (in press)
Staswick P (1982) Characterization of the subunits of glycinin and a complete primary structure of the A2B1A subunit. PhD Thesis Purdue University, West Lafayette Ind
Staswick PE, Hermodson MA, Nielsen NC (1981) Identification of the acidic and basic subunit complexes of glycinin. J Biol Chem 256:8752–8755
Staswick PE, Nielsen NC (1983) Characterization of a soybean cultivar lacking certain glycinin subunits. Arch Biochem Biophys 223:1–8
Staswick PE, Broue P, Nielsen NC (1983) Glycinin composition of several perennial species related to soybean. Plant Physiol 72:1114–1118
Thanh VH, Shibasaki K (1977) β-conglycinin from soybean proteins: isolation and immunological and physiochemical properties of the monomeric forms. Biochim Biophys Acta 490:370–384
Tumer NE, Thanh VH, Nielsen NC (1981) Purification and characterization of mRNA from soybean seeds: identification of glycinin and β-conglycinin precursors. J Biol Chem 256:8756–8760
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Communicated by D. von Wettstein
Cooperative research of USDA-ARS and the Indiana Agric. Exp. Stn., Purdue Univ., West Lafayette, IN 47907, USA. Indiana Agric. Exp. Stn. Journal Article 9675. Financial support from the American Soybean Association Research Foundation is gratefully acknowledged
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Kitamura, K., Davies, C.S. & Nielsen, N.C. Inheritance of alleles for Cgy1 and Gy4 storage protein genes in soybean. Theoret. Appl. Genetics 68, 253–257 (1984). https://doi.org/10.1007/BF00266899
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DOI: https://doi.org/10.1007/BF00266899