Summary
Effect of LH, vinblastine and cytochalasin B on the cytoskeleton of cultured Leydig cells was investigated using monoclonal antibodies and fluorescence microscopy. After LH addition and treatment with cytoskeletal disrupting drugs, three main effects were observed: 1) increase of androgen level secreted by cultured mouse Leydig cells, 2) changes of cell-shape towards regular and rounded, 3) increase of Δ5,3β-HSD activity. The results are discussed in respect to possible involvement of cytoskeleton in the regulation of steroidogenesis.
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Bilińska, B. Visualization of the cytoskeleton in Leydig cells in vitro. Histochemistry 93, 105–110 (1989). https://doi.org/10.1007/BF00266854
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DOI: https://doi.org/10.1007/BF00266854