Summary
In order to elucidate possible physiological roles of metallothionein (MT), we have studied immunohistological localization of MT in the eye of the rat, using an avidin-biotin peroxidase complex method. As a result, strong MT immunostaining was observed in the epithelium of the lens and cornea. In the retina, considerably strong MT immunostaining was observed in the pigment cell layer while the nerve fiber layer and inner plexiform layer showed weak MT staining. Glial cells in the optic nerve were found to have marked MT staining. The present result is consistent with the hypothesis that MT may be involved not only in activation of zinc enzymes and cell proliferation through supply of zinc ions, but also in a protective mechanism in the blood-retina barrier.
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References
Bhattacherjee P (1971) Distribution of carbonic anhydrase in the rabbit eye as demonstrated histochemically. Exp Eye Res 12:356–359
Bloom W, Fawcett DW (1986) A textbook of histology, 11th edn. Saunders, Philadelphia, p 955
Brady F, Webb M (1981) Metabolism of zinc and copper in the neonate (zinc,copper)-thionein in the developing rat kidney and testis. J Biol Chem 256:3931–3935
Bremner I (1987) Nutritional and physiological significance of metallothionein. In: Kägi JHR, Kojima Y (eds) Metallothionein II. Experientia, vol 52. Birkhäuser, Basel, pp 81–108
Danielson KG, Ohi S, Huang PC (1982) Immunochemical detection of metallothionein in specific epithelial cells of rat organs. Proc Natl Acad Sci USA 79:2301–2304
Goering PL, Fowler BA (1987) Activation of delta-aminolevulinic acid dehydratase following donation of zinc from kidney metallothionein. In: Kägi JHR, Kojima Y (eds) Metallothionein II. Experientia, vol 52. Birkhäuser, Basel, pp 613–616
Hanna C (1965) Changes in DNA, RNA and protein synthesis in the developing lens. Invest Ophthalmol 4:480–491
Hanna C, O'Brien JE (1960) Cell production and migration in the epithelial layer of the cornea. Arch Ophthalmol 64:536–539
Hanna C, Bicknell DS, O'Brien JE (1961) Cell turnover in the adult human eye. Arch Ophthalmol 65:695–698
Kägi JHR, Nordberg M (1978) Metallothionein: Experientia supplementum, vol 34. Birkhäuser, Basel
Karasawa M, Tohyama C, Nishimura N, Hashiba H, Kuroki T (1988) Possible role of metallothionein in epidermal hyperplasia. Proc 47th Ann Meet Jpn Cancer Assoc, 133 (abstract)
Karasawa M, Hashiba H, Tohyama C, Nishimura N, Kuroki T (1991) Possible role of metallothioneins in growth, differentiation and carcinogenesis of mouse skin. In: Klaassen CD, Suzuki KT (eds) Metallothionein in biology and medicine. Telford Press, Caldwell (USA), (in press)
Kissun RD, Graymore CN, Newhouse PJ (1972) Coenzyme dependency of alcohol dehydrogenase in the retina of the rat. II. Histochemistry. Exp Eye Res 14:150–153
Li T-Y, Kraker AJ, Shaw F III, Petering DH (1980) Ligand substitution reactions of metallothioneins with EDTA and apocarbonic anhydrase. Proc Natl Acad Sci USA 77:6334–6338
Lion F, Rotmans JP, Daemen FJM, Bonting SL (1975) Biochemical aspects of the visual process XXVII. Stereospecificity of ocular retinol dehydrogenases and the visual cycle. Biochim Biophys Acta 384:283–292
Mikulicich AG, Young RW (1963) Cell proliferation and displacement in the lens epithelium of young rats injected with tritiated thymidine. Invest Ophthalmol 2:344–354
Matsubara J, Tajima Y, Ikeda A, Kinoshita T, Shimoyama T (1988) A new perspective of radiation protection by metallothionein induction. Pharmacol Ther 39:331–333
Naganuma A, Satoh M, Imura N (1988) Specific reduction of toxic side effects of adriamycin by induction of metallothionein in mice. Jpn J Cancer Res (Gann) 79:406–411
Nishimura N, Nishimura H, Tohyama C, Karasawa M, Kuroki T (1988) Localization of metallothionein in epidermal hyperplasia caused by topical application of tumor promoter, 12-O-tetra decanoylphorbol-13-acetate, on the mouse skin. J Histochem Cytochem 36:945 (abstract)
Nishimura H, Nishimura N, Tohyama C (1989a) Immunohistochemical localization of metallothionein in developing rat tissues. J Histochem Cytochem 37:715–722
Nishimura N, Nishimura H, Tohyama C (1989b) Localization of metallothionein in female reproductive organs of rat and guinea pig. J Histochem Cytochem 37:1601–1607
Nishimura H, Nishimura N, Tohyama C (1990) Localization of metallothionein in the genital organs of the male rat. J Histochem Cytochem 38:927–933
Ohtake H, Hasegawa K, Koga M (1978) Zinc-binding protein in the livers of neonatal, normal and partially hepatectomized rats. Biochem J 174:999–1005
Roelfzema WH, Tohyama C, Nishimura H, Nishimura N, Morselt AWF (1989) Quantitative immunohistochemistry of metallothionein in rat placenta. Histochemistry 90:365–369
Takahashi T, Paliwal VK, Ebadi M (1988) Subcellular distribution of zinc and the presence of a metallothionein-like protein in bovine retina. Neurochem Int 13:525–530
Thornalley PJ, Vasak P (1985) Possible role for metallothionein in protection against radiation-induced oxidative stress. Kinetics and mechanism of its reaction with superoxide and hydrogen radicals. Biochim Biophys Acta 827:36–44
Tohyama C, Shaikh ZA (1978) Cross-reactivity of metallothioneins from different origins with rabbit anti-rat hepatic metallothionein antibody. Biochem Biophys Res Commun 84:907–913
Tohyama C, Shaikh ZA (1981) Metallothionein in plasma and urine of cadmium-exposed rats determined by a single-antibody radioimmunoassay. Fundam Appl Toxicol 1:1–7
Tohyama C, Nishimura H, Nishimura N (1988) Immunohistochemical localization of metallothionein in the liver and kidney of cadmium- or zinc-treated rats. Acta Histochem Cytochem 21:91–102
Tohyama C, Nishimura H, Nishimura N (1989) Localization of metallothionein in the regenerating liver from the rat. Proc 2nd Meet Int Soc Trace Element Res Humans 105, (abstract)
Tohyama C, Ghaffar A, Nakano A, Nishimura N, Nishimura H (1991) Immunohistochemical localization of metallothionein in organs of rats treated with either cadmium, inorganic or organic mercurials. In: Clarkson TW, Suzuki T, Imura N (eds) Advances in mercury toxicology. Plenum Publishing, New York (in press)
Udom AO, Brady FO (1980) Reactivation in vitro of zinc-requiring apo-enzymes by rat liver zinc-thionein. Biochem J 187:329–335
Umeyama T, Saruki K, Imai K, Yamanaka H, Suzuki K, Ikei N, Kodaira T, Nakajima K, Saitoh H, Kimura M (1987) Immunohistochemical demonstration of metallothionein in the rat prostate. Prostate 10:257–264
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Nishimura, H., Nishimura, N., Kobayashi, S. et al. Immunohistochemical localization of metallothionein in the eye of rats. Histochemistry 95, 535–539 (1991). https://doi.org/10.1007/BF00266738
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DOI: https://doi.org/10.1007/BF00266738