Summary
Experiments were conducted to determine the chromosomal location of the gene conditioning overproduction of a methionine-rich, 10-K zein in maize kernels of line BSSS53. In addition, the chromosomal location of the structural gene encoding the overproduced protein was determined. Whereas the structural gene, designated Zps10/(22), was found to be located on the long arm of chromosome 9 near the centromere, the locus regulating overproduction of the zein protein was mapped to the short arm of chromosome 4. This regulatory gene has been designated Zpr10/(22). Regulation of 10-K zein production by Zpr10/(22) is, therefore, via a trans-acting mechanism.
Similar content being viewed by others
References
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Bailey NTJ (1961) Introduction to the mathematical theory of genetic linkage. Oxford University Press, London
Binelli G, Soave C, Ottaviano E (1984) Location and differential expression of a zein gene in maize. Plant Sci Lett 33:259–265
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Das OP, Messing JW (1987) Allelic variation and differential expression at the 27-kilodalton zein locus in maize. Mol Cell Biol 7:4490–4497
Doggett H (1975) Progress in breeeding for quality protein in other cereals. In: Bauman LF, Carballo A, Mertz ET, Sprague EW (eds) High quality protein maize. Dowden, Hutchinson & Ross, Stroudsburg, pp 371–373
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Gianazza E, Viglienghi V, Righetti PG, Salamini F, Soave C (1977) Amino acid composition of zein molecular components. Phytochemistry 16:315–317
Hagen G, Rubenstein I (1981) Complex organization of zein genes in maize. Gene 13:239–249
Hastings H, Bonanomi S, Soave C, DiFonzo N, Salamini F (1984) Mapping of genes for minor zein SDS subunits and revision of zein genes nomenclature. Genet Agrar 38:447–464
Kirihara JA, Hunsperger JP, Mahoney WC, Messing JW (1988) Differential expression of a gene for a methionine-rich storage protein in maize. Mol Gen Genet 211:477–484
Kramer HH, Burnham CR (1947) Methods of combining linkage intensity values from backcross, F2, F3 genetic data. Genetics 32:379–390
Maier U-G, Brown JWS, Tolocyki C, Feix G (1987) Binding of a nuclear factor to a consensus sequence in the 5′ flanking region of zein genes from maize. EMBO J 6:17–22
Mertz ET, Bates LS, Nelson OE (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145:279–280
Paulis JW, Wall JS (1979) Note on mill for pulverizing single kernels of cereals for isoelectric focusing. Cereal Chem 56:497–498
Paulis JW, Wall JS, Sanderson J (1978) Origin of high methionine content in sugary-1 corn endosperm. Cereal Chem 55:705–712
Phillips, RL, McClure BA (1985) Elevated protein-bound methionine in seeds of a maize line resistant to lysine plus threonine. Cereal Chem 62:213–218
Righetti PG, Gianazza E, Viotti A, Soave C (1977) Heterogeneity of storage proteins in maize. Planta 136:115–123
Rubenstein I (1982) The zein multigene family. In: Sheridan WF (ed) Maize for biological research. University Press, University of North Dakota, Grand Forks, pp 189–196
Schmidt RJ, Burr FA, Burr B (1987) Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science 238:960–963
Shure M, Wessler S, Fedoroff N (1983) Molecular identification and isolation of the waxy locus in maize. Cell 35:225–233
Soave C, Salamini F (1983) Genetic organization and regulation of maize storage proteins. In: Daussant J, Mossé J, Vaughan J (eds) Seed proteins. Academic Press, London, pp 205–219
Soave C, Salamini F (1984) Organization and regulation of zein genes in maize endosperm. Philos Trans R Soc London Ser B 304:341–347
Soave C, Suman N, Viotti A, Salamini F (1978) Linkage relations between regulatory and structural gene loci involved in zein synthesis in maize. Theor Appl Genet 52:263–267
Thompson JF, Madison JT, Waterman MA, Muenster A-ME (1981) Effect of methionine on growth and protein composition of cultured soybean cotyledons. Phytochemistry 20:941–945
Wilson DR, Larkins BA (1984) Zein gene organization in maize and related grasses. J Mol Evol 20:330–340
Wilson CM, Sprague GF, Nelsen TC (1989) Linkages among zein genes determined by isoelectric focusing. Theor Appl Genet 77:217–226
Zar JH (1974) Biostatistical analysis. Prentice-Hall, Englewood Cliffs
Author information
Authors and Affiliations
Additional information
Communicated by F. Salamini
Rights and permissions
About this article
Cite this article
Benner, M.S., Phillips, R.L., Kirihara, J.A. et al. Genetic analysis of methionine-rich storage protein accumulation in maize. Theoret. Appl. Genetics 78, 761–767 (1989). https://doi.org/10.1007/BF00266655
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00266655