Summary
A method of localising oestrogen receptor in nuclei of breast cancer cells by the protein A-gold technique is described. Mast cell granules were found to take up uncoated particles of the gold sol selectively.
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Charpin C, Pierre-Marie M, Jacquemier J, Lavaut MN, Pourreau-Schneider N, Toga M (1986) Estrogen receptor immunocytochemical assay (ER-ICA): computerised image analysis system, immunoelectron microscopy and comparisons with estradiol binding assays in 115 breast carcinomas. Cancer Res 46:4271–4277 [Suppl]
Jackson P, Teasdale J, Cowen PN (1989) Development and validation of a sensitive immunohistochemical oestrogen receptor assay for use on archival breast cancer tissue. Histochemistry 92:149–152
Perrott-Applanat M, Groyer-Picard M-T, Logeat F, Milgrom E (1986) Ultrastructural localization of the progesterone receptor by an immunogold method: effect of hormone administration. J Cell Biol 102:1191–1199
Roth J (1984) The protein A-gold technique for antigen localisation in tissue sections by light and electron microscopy. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy, chap 9. Elsevier, Amsterdam, pp 113–121
Teasdale J, Jackson P, Holgate CS, Cowen PN (1987) Identification of oestrogen receptors in cells of paraffin-processed breast cancers by IGSS. Histochemistry 87:185–187
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Walsh, P.J., Teasdale, J. & Cowen, P.N. Ultrastructural localisation of oestrogen receptor in breast cancer cell nuclei. Histochemistry 95, 205–207 (1990). https://doi.org/10.1007/BF00266594
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DOI: https://doi.org/10.1007/BF00266594