Summary
Two borings (20 m depth) were performed in a sandy-clayey soil over a limestone bed and in a sandy soil with lumps of clay in some depths. Bacteria were found in the deeper soil layers of both profiles. The methods used to detect bacteria were those normally used for topsoil layers, plate counts of bacteria, ATP content, and direct microscopy. Measurements of CO2 evolution showed that the potential for bacterial activity was present in all depths of the two profiles. However, the activity was strongly dependent on the presence of easily available organic C. An indication of the denitrification potential was obtained by measuring the N2O evolution. Under aerobic incubation without the addition of glucose, N2O was detected only in the topsoil. When glucose was added to the soil samples, N2O was found at a low level in the deeper soil layers. Under anaerobic incubation, N2O was detected in all deeper layers, and increased markedly when glucose was added to the soil samples.
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References
Dunlap WJ, McNabb JF (1973) Subsurface biological activity in relation to ground-water pollution. Environmental Protection Agency Report EPA-660/2-73-014, Washington DC
Eiland F (1983) A simple method for quantitative determination of ATP in soil. Soil Biol Biochem 15:665–670
Eiland F (1985) Determination of adenosine triphosphate (ATP) and adenylate energy charge (AEC) in soil and use of adenine nucleotides as measures of soil microbial biomass and activity. Dissertation, Royal Veterinary and Agricultural University, Copenhagen
Greulich RH (1985) Groundwater contamination from oil: Behaviour control and treatment. Water Supply 3:233–242
Lind A-M (1985) Soil air concentration of N2O over 3 years of field experiments with animal manure and inorganic N-fertilizer. Dan J Plant Soil Sci 89:331–340
Lind A-M, Pedersen MB (1976a) Nitrate reduction in the subsoil: II. General description of borings profiles, and chemical investigations on the profile cores. Dan J Plant Soil Sci 80:82–99
Lind A-M, Pedersen MB (1976b) Nitrate reduction in the subsoil: III. Nitrate reduction experiments with subsoil samples. Dan J Plant Soil Sci 80:100–106.
Major DW, Mayfield CI, Barker JF (1988) Biotransformation of benzene by denitrifying in aquifer sand. Ground Water 26:8–14
Pedersen MB, Lind A-M (1976a) Nitrate reduction in the subsoil: I. Introductory studies of the nitrate reduction in the subsoil, and its influence on ground quality. Dan J Plant Soil Sci 80:73–81
Pedersen MB, Lind A-M (1976b) Nitrate reduction in the subsoil: IV. Some physical properties of the subsoil, their influence on chemical interchange in the soil, and on ground water quality. Dan J Plant Soil Sci 80:107–118
Smith MS, Thomas GW, White RE, Ritonga D (1985) Transport of Escherichia coli through intact and disturbed soil columns. J Environ Qual 14:87–91
Söderström BE (1977) Vital staining of fungi in pure cultures and in soil with fluorescein diacetate. Soil Biol Biochem 9:59–63
Tabatabai MA, Bremner JM (1970) Use of the Leco automatic 70-second carbon analyser for total carbon analysis of soils. Soil Sci Soc Am Proc 34:608–610
Trudell MR, Gillham RW, Cherry JA (1986) An in-situ study of the occurrence and rate of denitrification in a shallow unconfined sand aquifer. J Hydrol 83:251–268
Webster JJ, Hampton GJ, Wilson JT, Ghiorse WC, Beach FR (1985) Determination of microbial cell numbers in subsurface samples. Ground Water 23:17–23
Wilson JT, McNabb JF, Balkwill DL, Ghiorse WC (1983) Enumeration and characterization of bacteria indigenous to a shallow water-table aquifer. Ground Water 21:134–142
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Lind, A.M., Eiland, F. Microbiological characterization and nitrate reduction in subsurface soils. Biol Fert Soils 8, 197–203 (1989). https://doi.org/10.1007/BF00266479
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DOI: https://doi.org/10.1007/BF00266479