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The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD + hosts

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Summary

We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD + strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.

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References

  • Amundsen SK, Taylor AF, Chaudhury AM, Smith GR (1986) recD: the gene for an essential third subunit of Exonuclease V. Proc Natl Acad Sci USA 83:5558–5562

    Google Scholar 

  • Bachmann BJ (1990) Linkage map of Escherichia coli K-12, edition VIII. Microbiol Rev 54:130–197

    Google Scholar 

  • Barbour SD, Nagaishi H, Templin A, Clark AJ (1970) Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec − mutations. Proc Natl Acad Sci USA 67:128–135

    Google Scholar 

  • Berkowitz D, Hushon J, Whitfield H, Roth JR, Ames BN (1968) Procedure for identifying nonsense mutations. J Bacteriol 96:215–220

    Google Scholar 

  • Biek DP, Cohen SN (1986) Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. J Bacteriol 167:594–603

    Google Scholar 

  • Campbell A, Berg D, Lederberg E, Starlinger P, Botstein D, Novick R, Szybalski W (1977) Nomenclature of transposable elements in prokaryotes. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p 15

    Google Scholar 

  • Castilho BA, Olfson P, Casadaban MJ (1984) Plasmid insertion mutagenesis and lac gene fusion with mini-Mu bacteriophage transposons. J Bacteriol 158:488–495

    Google Scholar 

  • Chan RK, Botstein D, Watanabe T, Ogata Y (1972) Specialized transduction of tetracycline by phage P22 in Salmonella typhimurium. II Properties of a high frequency transducing lysate. Virology 50:883–898

    Google Scholar 

  • Chaudhury AM, Smith GR (1984) A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination. Proc Natl Acad Sci USA 81:7850–7854

    Google Scholar 

  • Chumley F, Menzel R, Roth JR (1979) Hfr formation directed by Tn10. Genetics 91:639–655

    Google Scholar 

  • Clark AJ (1973) Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet 7:67–86

    Google Scholar 

  • Clark AJ, Margulies AD (1965) Isolation and characterization of recombination-deficient mutants of Escherichia coli K-12. Proc Natl Acad Sci USA 53:451–459

    Google Scholar 

  • Cohen A, Clark AJ (1986) Synthesis of linear plasmid multimers in Escherichia coli K-12. J Bacteriol 167:327–335

    Google Scholar 

  • Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Demerec M, Alderberg E, Clark AJ, Hartman P (1966) A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61–76

    Google Scholar 

  • Dykstra CC, Prasher D, Kushner SR (1984) Physical and biochemical analysis of the cloned recB and recC gene of Escherichia coli K-12. J Bacteriol 157:21–27

    Google Scholar 

  • Eisenstark AR, Eisenstark J, van Dillewijn J, Rorsch A (1969) Radiation-sensitive and recombinationless mutants of Salmonella typhimurium. Mutat Res 8:497–504

    Google Scholar 

  • Elliott T, Roth JR (1988) Characterization of Tn10d-Cam: a transposition-defective Tn10 specifying chloramphenicol resistance. Mol Gen Genet 213:332–338

    Google Scholar 

  • Emmerson PT, Howard-Flanders P (1967) Cotransduction with thyA of a gene required for genetic recombination in Escherichia coli. J Bacteriol 93:1729–1731

    Google Scholar 

  • Enquist LW, Skalka A (1973) Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. J Mol Biol 75:185–212

    Google Scholar 

  • Fenton AC, Poteete AR (1984) Genetic analysis of the erf region of the bacteriophage P22 chromosome. Virology 134:148–160

    Google Scholar 

  • Fishel RA, James AA, Kolodner R (1981) recA-independent general genetic recombination of plasmids. Nature 294:184–186

    Google Scholar 

  • Horii ZI, Clark AJ (1973) Genetic analysis of the RecF pathway of genetic recombination in Escherichia coli K-12: isolation and characterization of mutants. J Mol Biol 80:327–334

    Google Scholar 

  • Hughes KT, Roth JR (1985) Directed formation of deletions and duplications using Mud(Ap, lac). Genetics 109:263–282

    Google Scholar 

  • Hughes KT, Roth JR (1988) Transitory cis complementation: a method for providing transposition functions to defective transposons. Genetics 119:9–12

    Google Scholar 

  • James AA, Morrison PT, Kolodner R (1982) Isolation of genetic elements that increase frequencies of plasmid recombinants. Nature 303:256–259

    Google Scholar 

  • Karu AE, Sakaki H, Echols H, Linn S (1975) The gam protein specified by bacteriophage lambda: structure and inhibitory activity for the recBC enzyme of Escherichia coli. J Biol Chem 250:7377–7387

    Google Scholar 

  • Kolodner R, Fishel RA, Howard M (1985) Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli. J Bacteriol 163:1060–1066

    Google Scholar 

  • Kushner SR, Nagaishi H, Templin A, Clark AJ (1971) Genetic recombination in Escherichia coli: the role of exonuclease I. Proc Natl Acad Sci USA 68:824–827

    Google Scholar 

  • Laban A, Cohen A (1981) Interplasmidic and intraplasmidic recombination in Escherichia coli. Mol Gen Genet 184:200–207

    Google Scholar 

  • Lloyd RC, Buckman C (1985) Identification and genetic analysis of sbcB mutations in commonly used recBC sbcB strains of Escherichia coli K-12. J Bacteriol 164:836–844

    Google Scholar 

  • Lloyd RC, Buckman C (1991) Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair. Biochimie 73:313–320

    Google Scholar 

  • Lloyd RC, Porton MC, Buckman C (1988) Effect of recF, recJ, recN, recO and ruv mutations on ultraviolet survival and genetic recombination in a recD strain of Escherichia coli K-12. Mol Gen Genet 212:317–324

    Google Scholar 

  • Lovett ST, Clark AJ (1984) Genetic analysis of the recJ gene of Escherichia coli K-12. J Bacteriol 157:190–196

    Google Scholar 

  • Lovett ST, Clark AJ (1985) Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins. J Bacteriol 162:280–285

    Google Scholar 

  • Lovett ST, Kolodner RD (1989) Identification and purification of a single-stranded DNA-specific exonuclease encoded by the recJ gene of Escherichia coli. Proc Natl Acad Sci USA 86:2627–2631

    Google Scholar 

  • Lovett ST, Kolodner RD (1991) Nucleotide sequence of the Escherichia coli recJ chromosomal region and construction of RecJ-overproducing plasmids. J Bacteriol 173:353–364

    Google Scholar 

  • Lovett ST, Luisi-Deluca C, Kolodner RD (1988) The genetic dependence of recombination in recD mutants of Escherichia coli. Genetics 120:37–45

    Google Scholar 

  • Mahan MJ, Roth JR (1989) recB and recC genes of Salmonella typhimurium. J Bacteriol 171:612–615

    Google Scholar 

  • Murphy KC, Fenton AC, Poteete AR (1987) Sequence of the bacteriophage P22 anti-recBCD (abc) genes and properties of P22 abc region deletion mutants. Virology 160:456–464

    Google Scholar 

  • Ochman H, Wilson AC (1987) Evolutionary history of enteric bacteria. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium: Cellular and molecular biology. American Society for Microbiology, Washington DC, p 1649

    Google Scholar 

  • Oliver DB, Goldberg EB (1977) Protection of parental T4 DNA from a restriction endonuclease by the product of gene 2. J Mol Biol 116:877–881

    Google Scholar 

  • Poteete AR, Volkert MR (1988) Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions. J Bacteriol 170:4379–4381

    Google Scholar 

  • Poteete AR, Fenton AC, Murphy KC (1988) Modulation of Escherichia coli RecBCD activity by the bacteriophage lambda gam and P22 Abe functions. J Bacteriol 170:2012–2021

    Google Scholar 

  • Sanderson KE, Roth JR (1988) Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev 52:485–532

    Google Scholar 

  • Schmieger H (1972) Phage P22 mutants with increased or decreased transduction abilities. Mol Gen Genet 119:75–88

    Google Scholar 

  • Smith GR (1983) Homologous recombination. In: Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (eds) Lambda II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p 175

    Google Scholar 

  • Smith GR (1988) Homologous recombination in prokaryotes. Microbiol Rev 52:1–18

    Google Scholar 

  • Templin A, Kushner SN, Clark AJ (1972) Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics 72:205–215

    Google Scholar 

  • Vogel H, Bonner D (1956) Acetylornithase of Escherichia coli: partial purification and some properties. J Biol Chem 218:97–106

    Google Scholar 

  • Weinstock GM (1987) General recombination in Escherichia coli. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium: Cellular and molecular biology. American Society for Microbiology, Washington DC, p 1034

    Google Scholar 

  • Willetts NS, Clark AJ, Low B (1969) Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol 97:244–249

    Google Scholar 

  • Williams JGK, Radding CM (1981) Partial purification and properties of an exonuclease inhibitor induced by bacteriophage Mu-1. Virology 39:548–588

    Google Scholar 

  • Zissler J, Signer ER, Schaefer F (1971) The role of recombination in the growth of bacteriophage lambda. In: Hershey A (ed) The bacteriophage lambda. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p 455

    Google Scholar 

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Author notes

  1. Michael J. Mahan

    Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., 02115, Boston, MA, USA

Authors and Affiliations

  1. Department of Biology, University of Utah, 84112, Salt Lake City, Utah, USA

    Michael J. Mahan & John R. Roth

  2. Departamento de Genética, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes, Apartado 1095, E-41080, Sevilla, Spain

    Josep Casadesus

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  1. Michael J. Mahan
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  2. Josep Casadesus
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Communicated by R. Devoret

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Mahan, M.J., Casadesus, J. & Roth, J.R. The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD + hosts. Molec. Gen. Genet. 232, 470–478 (1992). https://doi.org/10.1007/BF00266252

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  • DOI: https://doi.org/10.1007/BF00266252

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