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Expression of yeast cytochrome C1 is controlled at the transcriptional level by glucose, oxygen and haem

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Summary

The nuclear gene for cytochrome c 1 in Saccharomyces cerevisiae (CYT1) was localized on chromosome XV. Its upstream region was identified by functional complementation. Fusion to the lacZ reporter gene on a CEN plasmid allowed study of the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. Detailed promoter analysis combined with expression studies in recipient strains defective in regulatory genes identified cis-acting sites and transcription factors involved in the regulated expression of the cytochrome c 1 gene. These analyses showed that, in the presence of glucose, transcription of CYT1 is positively controlled by oxygen, presumably through the haem signal, and mediated by the HAP1-encoded transactivator. It is additionally regulated by the HAP2/3/4 complex which mediates gene activation mainly under glucose-free conditions. Basal transcription is, in part, effected by CPF1, a centromere and promoter-binding factor.

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Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie Maria-Ward-Strasse 1a, W-8000, München 19, Germany

    Ulrich Oechsner, Hannes Hermann, Alfred Zollner, Albert Haid & Wolfhard Bandlow

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  1. Ulrich Oechsner
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  2. Hannes Hermann
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  3. Alfred Zollner
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  4. Albert Haid
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  5. Wolfhard Bandlow
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Communicated by C.P. Hollenberg

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Oechsner, U., Hermann, H., Zollner, A. et al. Expression of yeast cytochrome C1 is controlled at the transcriptional level by glucose, oxygen and haem. Molec. Gen. Genet. 232, 447–459 (1992). https://doi.org/10.1007/BF00266250

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  • DOI: https://doi.org/10.1007/BF00266250

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