Summary
Five new elements of the mitotic control in the fission yeast Schizosaccharomyces pombe were isolated from gene libraries as multicopy suppressors of the conditional lethal phenotype of win1-1 weel ts cdc25ts triple mutant strains. These genes were designated wisl +−wis5+for win suppressing, and do not correspond to winl + or any of the previously characterised mitotic control genes. None of the wis genes is capable of suppressing the cdc phenotype of cdc25 ts strains, suggesting that their effect is not simply to reverse the effect of loss of cdc25 function. wisl + has been previously reported to encode a putative serine/threonine protein kinase that acts as a dosage-dependent inducer of mitosis. wis4 + appears to be a specific suppressor of the winl-1 mutation. wis2 + and wis3 + are capable of suppressing a wide range of cdc phenotypes arising from the combination of various mutations with wee1 ts and cdc25 ts, suggesting that the wis2 + and wis3 + products may interact with elements central to the mitotic control.
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Communicated by B. Kilbey
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Warbrick, E., Fantes, P.A. Five novel elements involved in the regulation of mitosis in fission yeast. Molec. Gen. Genet. 232, 440–446 (1992). https://doi.org/10.1007/BF00266249
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DOI: https://doi.org/10.1007/BF00266249